Características da gastrite crônica associada a Helicobacter pylori: aspectos topográficos, doenças associadas e correlação com o status cagA

Álvares, Mônica; Marino, Marília Campos Abreu; Oliveira, Celso Affonso de; Mendes, Cláudia Castro; Costa, Adriana; Guerra, Juliana Becattini; Queiroz, Dulciene Maria Magalhães; Nogueira, Ana Margarida Miguel Ferreira

doi:10.1590/s1676-24442006000100011

Cited by 9 publications

(12 citation statements)

References 31 publications

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“…An investigation in other developing countries also showed a similar frequency (61.8%) [ 30 ]. In adults, a slightly higher frequency was found in other studies in Brazilian (88.4%) [ 31 ] and African (70–97%) populations [ 32 ]. These numbers most likely reflect the social and health conditions of the studied populations because infections by HP are more common in developing countries than in developed countries [ 19 ].…”

Section: Discussionmentioning

confidence: 51%

Occurrence of Helicobacter pyloriand Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil

et al. 2014

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BackgroundHelicobacter pylori (HP) and Epstein-Barr virus (EBV) have been associated with cancer development. We evaluated the prevalence of HP, HP CagA+ and EBV infection in gastric cancer (GC) samples from adults and in gastric tissues from patients who underwent upper endoscopy (UE).MethodsSamples from UE and GC were collected to investigate the presence of HP infection and the HP virulence factor CagA by a urease test and PCR. The presence of EBV was detected by Eber-1 in situ hybridization.ResultsIn UE, 85.5% of juvenile patients showed some degree of gastritis (45.3% of patients with mild gastritis and 54.7% with moderate/severe gastritis) and patients with mild gastritis were younger than patients with moderate/severe gastritis. Among adults, 48.7% presented mild gastritis and 51.3% moderate/severe gastritis. HP infection was detected in 0% of normal mucosa, 58.5% of juvenile gastritis patients, 69.2% of adult gastritis patients and 88% of GC patients. In these same groups, HP CagA+ was detected in 0%, 37.7%, 61.5% and 67.2% of tissue samples, respectively. In juvenile patients, HP infection was more common in those with gastritis than in normal samples (p = 0.004). The patients with either HP or HP CagA+ were older than patients without these pathogens (p < 0.05). In juvenile patients, HP infection was more frequent in cases of moderate/severe gastritis than in cases of mild gastritis (p = 0.026). Moreover, in patients with GC, HP infection was more frequent in males than in females (p = 0.023). GC patients with HP CagA+ were older than patients with HP CagA- (p = 0.027). HP CagA+ was more common in intestinal-type than diffuse-type GC (p = 0.012). HP CagA+ was also associated with lymph-node (p = 0.024) and distal (p = 0.005) metastasis. No association between EBV infection and HP infection or any clinicopathological variable was detected.ConclusionsOur results suggest that HP is involved in the pathophysiology of severe gastric lesions and in the development of GC, particularly when CagA+ is present. EBV was not the primary pathogenic factor in our samples.

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Section: Discussionmentioning

confidence: 51%

Occurrence of Helicobacter pyloriand Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil

et al. 2014

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“…Several studies have demonstrated that infections caused by cagA -positive H. pylori strains are associated with greater intensity of intestinal inflammation, activity, atrophy and metaplasia (1). According to Parsonnet and collaborators, patients infected by these strains would have a three times greater risk of developing gastric cancer (15).…”

Section: Discussionmentioning

confidence: 99%

Characterization of virulence genes cagA and vacA in Helicobacter Pylori and their prevalence in gastrointestinal disorders

Cogo

Monteiro

Nogueira

et al. 2011

Braz. J. Microbiol.

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Prevalence of H. pylori infection was determined using cultures of gastric biopsy samples of patients attended at the academic hospital of the Federal University of Paraná, Curitiba, Paraná, Brazil. Molecular methods were used to characterize the cagA and vacA genes from bacterial isolates associated with different diseases presented by patients. Out of a total of 81, forty-two gastric biopsy samples tested were positive for H. pylori, with a prevalence of 51.9%. No significant difference was found with regard to the gender (p=0.793) and age (p=0.183) of the patients. Genotype s1m1 vacA gene was found in 67% of the cases of peptic ulcer investigated (p=1.0), despite the limited number of patients with this disease (n=3). A correlation between the presence of less virulent strains (s2m2) and reflux esophagitis was found in the majority of the cases (45%), but without statistical significance. An association between the prevalence of cagA gene, found in 92% of isolates, and peptic ulcer was not observed (p=1.0), suggesting that this gene cannot be considered a specific marker of severity in our environment. The results reinforce the importance of conducting regional studies and the need to characterize H. pylori virulence genes associated with different diseases.

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“…The FOX test system was based on ferrous ion (Fe +2 ) to ferric ion (Fe +3 ) transformation by various types of peroxides in the samples analyzed. A purple-blue color complex (ferric-xylenol orange complex) was formed in the presence of ferric-xylenol, whose absorbance could be measured (Costa et al 2006). A special reagent that was prepared on the day of the analysis (the FOX reagent) is used in this method: solution A was prepared with 7.6 mg of xylenol orange and 88 mg of butylhydroxytoluene (BHT) in 90 mL of methanol; solution B was prepared with 9.8 mg of ferrous sulfate in 10 mL of 250 mM sulfuric acid (0.13 mL sulfuric acid in 10 mL water).…”

Section: Determination Of Total Hydroperoxide Concentration (Fox)mentioning

confidence: 99%

“…After this reading, a 25 µL aliquot of the stock solution of DTNB (10 nmol/L in absolute ethanol) was added to the solution, which was kept at room temperature for 15 min. After this period, a new reading was obtained using DTNB as the blank (Costa et al 2006). The concentration of sulfhydryl groups was calculated using GSH, and the result was reported as nmol/g protein.…”

Section: Determination Of Gsh Concentration -Total Concentration Of Tmentioning

confidence: 99%

Oxidative stress and polycystic ovary syndrome: an evaluation during ovarian stimulation for intracytoplasmic sperm injection

et al. 2017

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Oxidative stress (OS) may affect natural fertility and the results of assisted reproduction techniques (ARTs). Subfertility associated with polycystic ovary syndrome (PCOS) may be related to OS. This process may intensify during controlled ovarian stimulation (COS) for ARTs because of increased ovarian metabolic activity and hypoestrogenism with the use of gonadotropin-releasing hormone agonists (GnRHas). The objective of this study was to investigate the presence of systemic OS in non-stimulated cycles and to determine OS markers (malondialdehyde [MDA], advanced oxidation protein products [AOPP], hydroperoxides [FOX], glutathione [GSH], and vitamin E) during COS in non-obese infertile women with and without PCOS who were subjected to ARTs. A prospective cohort study was conducted on non-obese women (16 with PCOS, and 60 ovulatory patients with infertility due to male and/or tubal factors). The OS markers were determined during the following time-points: the follicular phase of the natural cycle (D1), after pituitary downregulation with GnRHa and before the use of gonadotropins (D2), on the day of administration of human chorionic gonadotropin (D3), and at oocyte retrieval (D4). Intergroup analysis showed that serum MDA concentrations were higher in the PCOS group at D3 (P=0.048) and D4 (P=0.002). On an intragroup analysis, the control group had higher MDA concentrations at D2 than at D1 (P=0.01) or D4 (P=0.004). The AOPP concentrations were higher at D2 (P<0.0001), D3 (P<0.001) and D4 (P<0.0001) compared to D1. The FOX concentrations were lower at D2 (P<0.0001), D3 (P<0.0001), and D4 (P<0.001) than at D1. Serum GSH concentrations were significantly higher at D4 than at D1 (P=0.02). An intragroup analysis of the PCOS subjects showed that the five OS markers did not differ significantly among the four time-points when they were analyzed (D1, D2, D3 and D4). In conclusion, non-obese infertile women with PCOS showed evidence of systemic OS after COS with gonadotropins for ICSI. On the other hand, non-obese ovulatory infertile women, and women with infertility due to male and/or tubal factors showed a possible systemic oxidative balance until the final of COS.

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Características da gastrite crônica associada a Helicobacter pylori: aspectos topográficos, doenças associadas e correlação com o status cagA

Abstract: Helicobacter pylori infection Chronic gastritis cagA-positive strains Pathogenesis

Cited by 9 publications

References 31 publications

Occurrence of Helicobacter pyloriand Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil

Occurrence of Helicobacter pyloriand Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil

Characterization of virulence genes cagA and vacA in Helicobacter Pylori and their prevalence in gastrointestinal disorders

Oxidative stress and polycystic ovary syndrome: an evaluation during ovarian stimulation for intracytoplasmic sperm injection

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