Kalanchoe daigremontiana is valued for its antioxidant and antimicrobial properties, mainly due to bioactive compounds like phenolic acids, including ferulic acid. Endophytic fungi can act as elicitors in tissue culture, enhancing compound production, promoting growth, and improving microbial control. This study aims to evaluate the compound profile, growth promotion, and antibacterial activity of K. daigremontiana plantlets grown in vitro in a culture medium supplemented with an antioxidant endophytic fungus from K. daigremontiana used as an elicitor. Sixteen endophytic fungal strains were recovered from mineral oil preservation and tested for antioxidant activity using the DPPH• (2,2-diphenyl-1-picrylhydrazyl) assay, with Alternaria alternata (strain 16) showing the highest activity. Identified through macroscopic, microscopic, and molecular methods, this strain was cultured and used as a biotic elicitor in Murashige-Skoog medium for K. daigremontiana explants. Extracts from elicited and non-elicited in vitro-cultivated plantlets, as well as non-elicited in vivo-cultivated plantlets, were analyzed by high-performance liquid chromatography for phenolic compounds and tested for antibacterial activity using microdilution method. Elicitation enhanced plantlet growth and altered the compound profile, with ferulic acid found exclusively in in vitro plantlets. Elicited in vitro plantlets showed the strongest antibacterial activity against Escherichia coli and Staphylococcus aureus. These results suggest that in vitro elicitation improves the growth and antibacterial properties of K. daigremontiana, offering a potential strategy to enhance bioactive compound production, particularly ferulic acid. This approach could be an alternative to manage systemic phytopathogens in vivo, particularly non-culturable ones, in other plants.