Caracterização morfológica de isolados de Cryptosporiopsis perennans em diferentes meios de cultura

Bogo, Amauri; Maffioletti, Marta A.; Sanhueza, Rosa Maria Valdebenito; Casa, Ricardo Trezzi

doi:10.1590/s1982-56762008000300013

Cited by 10 publications

(6 citation statements)

References 7 publications

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“…This applies to Curvularia sp., which can grow in the presence or absence of light. The same characteristic was reported for Fusarium moniliforme Sheld, whose mycelial growth remains the same irrespective of the presence of a light source (Teixeira et al, 2001 (Bogo et al, 2008). S. diffusum could be considered a slow growth since it takes around two months to form a colony of about 30 mm (Table 3).…”

Section: Discussionsupporting

confidence: 70%

Culture Medium, Light Regime and Temperature Affect the Development of Sirosporium diffusum

Poletto¹,

Muniz²,

Fantinel³

et al. 2018

JAS

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Sirosporium diffusum is the causal agent of the brown leaf spot disease on pecan trees that seriously damages the foliage of adult plants and seedlings. This fungal species is difficult to grow satisfactorily in a culture medium. Therefore, the aim of this study was to evaluate the effects of different physical conditions on the development of S. diffusum. In the first assay, eight culture media and five light regimes were combined, while in the second, the three treatments that promoted highest sporulation were combined with three temperatures. The trials were conducted in a two-factorial arrangement in a fully randomized design with six replicates. V8, V8CaCO 3, and CA media under a 24-h photoperiod produced the highest respective sporulations: 29 × 10 4 , 35 × 10 4 , and 41 × 10 4 conidia ml -1. The best temperature for sporulation was 20±1 °C for all culture media, especially V8CaCO 3 and CA. The best artificial conditions for obtaining good mycelial growth and sporulation consisted of a photoperiod of 24 h, temperature of 20±1 °C and V8CaCO 3 or CA culture medium.

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Section: Discussionsupporting

confidence: 70%

Culture Medium, Light Regime and Temperature Affect the Development of Sirosporium diffusum

Poletto¹,

Muniz²,

Fantinel³

et al. 2018

JAS

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“…The differences observed in the AMG and MGI of the isolates on PDA, AM, and SN may have been due to qualitative and quantitative aspects of these media. PDA has a simple formulation and the highest nutrient contents, supporting the best mycelial growth of the fungus (BOGO et al, 2008;SAHA et al, 2008). The genera Neonectria and Cylindrocarpon usually produce pigmentation with tones of light brown and white borders on potato sucrose agar medium, but on SN medium the colonies Isolates were not pigmented (hyaline) and the mycelium was aerial (LANGRELL, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…(CASTLEBURY et al, 2006;CHAVERRI et al, 2011;WEBER, 2014). The causal agent was originally named Nectria ditissima in 1865 (FLACK & SWINBURNE, 1977) and is already well spread worldwide (SANHUEZA, 1998;BOGO et al, 2008). In Brazil, EC was first reported in a nursery in Vacaria municipality in the state of Rio Grande do Sul at 1998(SANHUEZA, 1998.…”

Section: Introductionmentioning

confidence: 99%

European apple canker: morphophysiological variability and pathogenicity in isolates of Neonectria ditissima in southern Brazil

et al. 2017

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European apple canker (EC) is caused by Neonectria ditissima, a pathogen officially registered as a quarantine pest in 2012. Thirty-five isolates of N. ditissima of different geographical regions of southern Brazil from apple branches showing symptoms of EC were identified by the specific pair primers Ch1 and Ch2 and analyzed concerning the virulence on Gala apple cultivar and morphophysiological characteristics. The disease symptoms were characterized and the isolates compared based on average mycelium growth (AMG), mycelium growth index (MGI), colony color, conidia type, dimensions and growth on potato dextrose agar (PDA), malt agar (AM), and synthetic SNAY (SN) culture media. Nineteen isolates showed the greatest AMG on PDA, forming three growth groups of 35.56 (GI), 52.71 (GII), and 62.67mm (GIII). Seven isolates showed MGI greater than 4.0mm diameter on PDA compared with that on AM and SN. The highest conidia production was on SN, and the predominant colony color in all media was white to beige with central pigmentation of brown and borders colored in shades of beige. There were significant differences among the average dimensions of micro- and macroconidia on PDA, AM, and SN. The pathogenicity was confirmed for all isolates despite of different morphophysiological characteristics. There was no correlation among isolates morphophysiological variability, virulence, and geographical origin.

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“…In relation to the culture media, the highest mycelial growth rates for P. cedrelae occurred on MEA and V8 juice agar. Bogo, Maffioletti, Valdebenito‐Sanhueza, and Casa () also reported more rapid colony development in these two culture media for Cryptosporiopsis perennans . The composition and nutrient concentration in the substrate, the nutritional requirements and the physiological variability of the isolate, in addition to environmental conditions, all influence the growth of fungi (Cruz, Prestes, & Maciel, ).…”

Section: Discussionmentioning

confidence: 80%

Epitypification, characterization and phylogenetic positioning of Pseudobeltrania cedrelae, the causal agent of pseudobeltrania spot on Cedrela fissilis

et al. 2018

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Summary The fungus Pseudobeltrania cedrelae, the type species of the genus, is the causal agent of an important leaf spot in seedlings and adult plant of cedar (Cedrela fissilis). Due to the contradictory phylogenetic position of the genus Pseudobeltrania, epitypification of P. cedrelae was carried out based on a culture obtained from the same locality and host of the original type. Samples were collected, and 10 isolates of P. cedrelae associated with lesions on cedar leaflets were obtained. For morphological characterization, conidia, conidiophores, conidiogeneous cells, conidiogeneous loci and basal cells were taken both from the fungus obtained from leaf lesions and from that obtained in slide culture. Mycelial growth rates and sporulation were evaluated in six different culture media. For molecular phylogeny, maximum parsimony analyses were performed from the ITS and 28S sequences of the isolates. Both the morphological characteristics of the fungal structures obtained from symptomatic leaves and the slide culture technique presented variations. In foliar lesions, isolates presented the same morphological characteristics as the type material. Mycelial growth rate and sporulation of P. cedrelae were greatest on malt extract agar and V8 juice agar. Pseudobeltrania cedrelae was pathogenic when inoculated into healthy cedar plants. According to the phylogenetic tree, isolates grouped in the same clade, but in a distinct clade of Pseudobeltrania ocoteae. The results suggested that P. ocoteae belongs to the genus Hemibeltrania. This paper presents new information on P. cedrelae that contributes to clarifying the phylogeny of the Beltraniaceae.

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Caracterização morfológica de isolados de Cryptosporiopsis perennans em diferentes meios de cultura

Cited by 10 publications

References 7 publications

Culture Medium, Light Regime and Temperature Affect the Development of Sirosporium diffusum

Culture Medium, Light Regime and Temperature Affect the Development of Sirosporium diffusum

European apple canker: morphophysiological variability and pathogenicity in isolates of Neonectria ditissima in southern Brazil

Epitypification, characterization and phylogenetic positioning of Pseudobeltrania cedrelae, the causal agent of pseudobeltrania spot on Cedrela fissilis

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