Caracterización de aislamientos nativos de Beauveria bassiana y su patogenicidad hacia Hypothenemus hampei, en Tabasco, México

Gerónimo-Torres, José del Carmen; Torres-de-la-Cruz, Magdiel; Cruz, Manuel Pérez-de-la; De-la-Cruz-Pérez, Aracely; Ortiz-García, Carlos Fredy; Cappello-García, Silvia

doi:10.25100/socolen.v42i1.6666

Cited by 9 publications

(10 citation statements)

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“…with 3.83 × 10 8 conidia/mL −1 . Te conidia production obtained by B. bassiana and B. peruviensis is close to values reported by Torres et al [29] for native strains of B. bassiana in Tabasco, Mexico. However, the conidia production obtained with Metarhizium sp.…”

Section: Resultssupporting

confidence: 89%

“…Te mortality caused by B. bassiana 1 × 10 9 and B. bassiana 1 × 10 7 on H. hampei agrees with Bastidas et al [35] who reported 90% mortality of H. hampei caused by B. bassiana strains at times from 106 to 129 h. Likewise, Torres et al [29] reported 100% mortality of H. hampei by native strains of B. bassiana in Mexico. However, these authors reported mortality at 144 h and a concentration of 1 × 10 7 .…”

Section: Resultssupporting

confidence: 86%

“…In the two trials, the treatments with the highest consistency in both LT 50 and LT 90 were B. bassiana 1 × 10 9 conidia/mL −1 and B. peruviensis 1 × 10 9 conidia/mL −1 . Tese results agree with Torres et al [29] who reported LT 50 from 71 to 103 h and LT 90 from 91 to 132 h in B. bassiana strains in Mexico, at a dose of 1 × 10 7 conidia/mL −1 . However, in our study, the dose of 1 × 10 9 conidia/mL −1 reduced LT 50 to values from 60 to 62 h. On the other hand, López-Blanco [39] reported LT 50 of 57 to 74 h for B. bassiana strains on H. hampei, when using a dose of 1 × 10 7 conidia/mL −1 .…”

Section: Lethal Time 50 and 90 (Lt 50 And Lt 90 )supporting

confidence: 91%

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In Vitro Biological Activity of Beauveria bassiana, Beauveria peruviensis, and Metarhizium sp. against Hypothenemus hampei (Coleoptera: Curculionidae)

Chuquibala-Checan,

Torres-de la Cruz,

Leiva

et al. 2023

International Journal of Agronomy

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Coffee (Coffea arabica) is the main commodity in Peru and is the economic support for thousands of small farmers. However, coffee production is affected by the coffee berry borer (Hypothenemus hampei). Currently, H. hampei is the most important pest in whole coffee-growing regions in Peru. This study aimed to evaluate in vitro biological activity of Beauveria bassiana, Beauveria peruviensis, and Metarhizium sp. against Hypothenemus hampei in two trials at different times. Conidia production, Conidia viability, and pathogenicity against H. hampei were evaluated at three concentrations (1 × 105, 1 × 107, and 1 × 109 conidia/mL−1). In addition, lethal times (LT50 and LT90) and lethal concentrations (LC50 and LC90) were calculated. There were significant differences in conidia production ( P < 0.001) and conidia viability ( P < 0.041). The highest conidia production and conidia viability were reached by B. bassiana and B. peruviensis, respectively. Likewise, there were differences in the pathogenicity of the strains in the two tests carried out (test 1: P < 0.0009 and test 2: P < 0.0001). The highest mortality occurred in the treatments of B. bassiana 1 × 109 conidia/mL−1, B. bassiana 1 × 107 conidia/mL−1, and B. peruviensis 1 × 109 conidia/mL−1. The treatments with lower LT50 and LT90 were B. bassiana 1 × 109 conidia/mL−1 and B. peruviensis 1 × 109 conidia/mL−1, and the strains with the lowest LC50 and LC90 were B. peruviensis and B. bassiana. The in vitro characteristics shown by B. bassiana and B. peruviensis conditions suggest they should be evaluated in the field to determine the capability of these strains to reduce populations of H. hampei.

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Section: Resultssupporting

confidence: 89%

Section: Resultssupporting

confidence: 86%

Section: Lethal Time 50 and 90 (Lt 50 And Lt 90 )supporting

confidence: 91%

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In Vitro Biological Activity of Beauveria bassiana, Beauveria peruviensis, and Metarhizium sp. against Hypothenemus hampei (Coleoptera: Curculionidae)

Chuquibala-Checan,

Torres-de la Cruz,

Leiva

et al. 2023

International Journal of Agronomy

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“…Fungal strains were isolated from infected coffee borers ( Hypothenemus hampei ) obtained from infected coffee berries according to Gerónimo-Torres et al (2016). They were collected during July and August 2017 from three districts in the province of Rodriguez de Mendoza, Amazonas, Peru (Fig.…”

Section: Methodsmentioning

confidence: 99%

Phylogeny and species delimitations in the entomopathogenic genus Beauveria (Hypocreales, Ascomycota), including the description of B. peruviensis sp. nov.

Bustamante¹,

Oliva²,

Leiva³

et al. 2019

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The genus Beauveria is considered a cosmopolitan anamorphic and teleomorphic genus of soilborne necrotrophic arthropod-pathogenic fungi that includes ecologically and economically important species. Species identification in Beauveria is difficult because of its structural simplicity and the lack of distinctive phenotypic variation. Therefore, the use of multi-locus sequence data is essential to establish robust species boundaries in addition to DNA-based species delimitation methods using genetic distance, coalescent, and genealogical concordance approaches (polyphasic approaches). In this regard, our study used multilocus phylogeny and five DNA-based methods to delimit species in Beauveria using three molecular makers. These polyphasic analyses allowed for the delimitation of 20–28 species in Beauveria, confirming cryptic diversity in five species (i.e. B. amorpha, B. bassiana, B. diapheromeriphila, and B. pseudobassiana) and supporting the description of B. peruviensis as a new taxon from northeastern Peru. The other five species were not evaluated as they did not have enough data (i.e. B. araneola, B. gryllotalpidicola, B. loeiensis, B. medogensis, and B. rudraprayagi). Our results demonstrate that the congruence among different methods in a polyphasic approach (e.g. genetic distance and coalescence methods) is more likely to show reliably supported species boundaries. Among the methods applied in this study, genetic distance, coalescent approaches, and multilocus phylogeny are crucial when establishing species boundaries in Beauveria.

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“…According to Mota et al (2017), fungal species B. bassiana used in synthetic tissues surface treatment, at concentration of 5.44 × 10 8 .cm -2 , recorded 88.5% H. hampei mortality rate after five seconds of exposure to it. Immersion based studies conducted with different B. bassiana isolates against H. hampei recorded up to 100% insect mortality rate, Gerónimo-Torres et al (2016); Posada-Flórez (2008).…”

Section: Introductionmentioning

confidence: 99%

Comparision of different application methods to biological control Hypothenemus hampei

Pereira

Almeida

Filho

2021

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There are several studies that deal with the problem of infestation by Hypothenemus hampei in Coffeea arabica. However, the ways of applying products based on entomopathogenic fungi are the same as those used for chemicals. The objective of the research was to comparison different methods of application of Beauveria bassiana against H. hampei. The experiments were developed in laboratory, with blastospores and B. bassiana airborne conidia powders were carried out. Application methods based on immersion, Potter Spray Tower and walking on treated surface. Were three experiments were carried out for each method. Bioassays'design comprised seven treatments (including the control), with five repetitions (each) and 25 beetles per repetition. Suspensions were standardized at 2.4×10 9 , 1×10 9 , 5×10 8 , 1×10 8 , 5×10 7 and 1×10 7 conidia.mL -1 . Conidia powder presented initial concentration of 1.14×10 10 and it was standardized at 1.14×10 9 ; 5.7×10 9 ; 1.14×10 8 ; 5.7×10 7 ; 1.14×10 7 and 5.7×10 6 conidia.g -1 . A simultaneous test was also carried out based to the three application methods; treatment comprised conidia powder concentration of 1x10 8 and the control. Data were subjected to analysis of variance, which was followed by Tukey test (P<0.05) and, CL 50 , in the SPSS 12.1 software. The mortality better rates of H. hampei exposed to B. bassiana IBCB66 were1×10 8 (immersion), 1×10 9 (Potter Spray Tower) and 1×10 8 (walking), where as mean LC 50 were 6.1×10 8 , 4.1×10 8 , 2.4×10 8 conidia.mL -1 for each method, respectively. The highest mortality rate was recorded in the walking tests on the conidian powder at a concentration of 1×10 8 conidia.g -1 for the three repetitions of the method and also in the simultaneous test.

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Caracterización de aislamientos nativos de Beauveria bassiana y su patogenicidad hacia Hypothenemus hampei, en Tabasco, México

Cited by 9 publications

References 0 publications

In Vitro Biological Activity of Beauveria bassiana, Beauveria peruviensis, and Metarhizium sp. against Hypothenemus hampei (Coleoptera: Curculionidae)

In Vitro Biological Activity of Beauveria bassiana, Beauveria peruviensis, and Metarhizium sp. against Hypothenemus hampei (Coleoptera: Curculionidae)

Phylogeny and species delimitations in the entomopathogenic genus Beauveria (Hypocreales, Ascomycota), including the description of B. peruviensis sp. nov.

Comparision of different application methods to biological control Hypothenemus hampei

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