Carbachol improves secretion in the early phase after rabbit submandibular gland transplantation

Abstract

A lack of acetylcholine and downregulation of the muscarinic receptor signaling pathway is involved in the early hypofunction of transplanted SMGs. Carbachol treatment could be a new therapeutic strategy to improve secretion and prevent the obstruction of Wharton's duct in the early phase after SMG transplantation.

Obstructive sialadenitis of a transplanted submandibular gland: chronic inflammation secondary to ductal obstruction

“…SMGs of rabbits that did not undergo transplantation showed mixed glands that contained more mucous acini (figure 3A), as reported previously 15. Similar morphological manifestations were observed in the transplanted glands with normal levels of secretion (figure 3B).…”

“…A tube was inserted into Wharton's duct and the length of filter paper (35 mm×5 mm) moistened by the secretion from the tube was recorded for 5 min, as described previously 14 15…”

Botulinum toxin A inhibits salivary secretion of rabbit submandibular gland

“…The sense and antisense primers for M3-muscarinic acetylcholine receptor (M3 receptor) (NM_000740), AQP5 (AF_495879) and β-actin were 5′-CTT CTC CAA GCT TCC CAT CCA-3′ and 5′-TGA CCG ACT GTC TCT GCT GGT-3′, 5′-GTT CCT GGC CAC CCT CAT CT-3′ and 5′-ACA GAC AGG CCG ATG GAC AG-3′, and 5′-ATC TGG CAC CAC ACC TTC TAC AAT GAG CTG GCG-3′ and 5′-CGC CAT ACT CCT GCT TGC TGA TCC ACA TCT GC-3′, respectively. 15 β-actin was amplified as the internal standard. The amplification products were visualized on 1.5% agarose gel with ethidium bromide and sequenced to confirm their identities.…”

An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits

“…Therefore, the efficacy and safety of BTXA should be well demonstrated in animal models before we could use it in patients. Considering that the previous models were intricate and did not well reflect the truth of salivary secretion [21, 22, 25, 37], we established a new transplanted SMG model in rabbits. In this model, we injected 10 U BTXA (100 U/mL) into the transferred gland and found BTXA also inhibited the salivary secretion temporarily.…”