Carbohydrate accumulation and utilization by oocytes of <i>Rhodnius prolixus</i>

Santos, Rachel; Mariano, Ana; Rosas-Oliveira, Rafael; Pascarelli, Bernardo Miguel de Oliveira; Machado, Ednildo A.; Meyer‐Fernandes, José Roberto; Gondim, Kátia C.

doi:10.1002/arch.20217

Cited by 34 publications

(17 citation statements)

References 27 publications

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“…Glycogen content (mg glucose/g total protein) was measured as described by Santos et al (2008), using 100 μl of the supernatant . These were incubated for 4 h at 37 °C in the presence of 20 μl (1 U) amyloglucosidase (EC 3.2.1.3, Sigma, Darmstadt, Germany) diluted in 100 mM sodium acetate (pH 5.5) to hydrolyze glycogen.…”

Section: Methodsmentioning

confidence: 99%

Glycogen Phosphorylase and Glycogen Synthase: Gene Cloning and Expression Analysis Reveal Their Role in Trehalose Metabolism in the Brown Planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae)

Zhang¹,

Wang²,

Chen³

et al. 2017

Journal of Insect Science

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RNA interference has been used to study insects’ gene function and regulation. Glycogen synthase (GS) and glycogen phosphorylase (GP) are two key enzymes in carbohydrates’ conversion in insects. Glycogen content and GP and GS gene expression in several tissues and developmental stages of the Brown planthopper Nilaparvata lugens Stål (Hemiptera: Delphacidae) were analyzed in the present study, using quantitative reverse-transcription polymerase chain reaction to determine their response to double-stranded trehalases (dsTREs), trehalose-6-phosphate synthases (dsTPSs), and validamycin injection. The highest expression of both genes was detected in the wing bud, followed by leg and head tissues, and different expression patterns were shown across the developmental stages analyzed. Glycogen content significantly decreased 48 and 72 h after dsTPSs injection and 48 h after dsTREs injection. GP expression increased 48 h after dsTREs and dsTPSs injection and significantly decreased 72 h after dsTPSs, dsTRE1-1, and dsTRE1-2 injection. GS expression significantly decreased 48 h after dsTPS2 and dsTRE2 injection and 72 h after dsTRE1-1 and dsTRE1-2 injection. GP and GS expression and glycogen content significantly decreased 48 h after validamycin injection. The GP activity significantly decreased 48 h after validamycin injection, while GS activities of dsTPS1 and dsTRE2 injection groups were significantly higher than that of double-stranded GFP (dsGFP) 48 h after injection, respectively. Thus, glycogen is synthesized, released, and degraded across several insect tissues according to the need to maintain stable trehalose levels.

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Section: Methodsmentioning

confidence: 99%

Glycogen Phosphorylase and Glycogen Synthase: Gene Cloning and Expression Analysis Reveal Their Role in Trehalose Metabolism in the Brown Planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae)

Zhang¹,

Wang²,

Chen³

et al. 2017

Journal of Insect Science

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“…Mobilization of egg components has also been the subject of several reports ( Abreu et al. 2008 ; Santos et al. 2008 , 2011 ).…”

Section: Discussionmentioning

confidence: 97%

“…Previous reports have noted that glycogen is consumed as the embryo develops ( Regano-Caracciolo et al. 1998 , Santos et al. 2008 ).…”

Section: Discussionmentioning

confidence: 99%

Embryonic Development of Rhynchophorus palmarum (Coleoptera: Curculionidae): Dynamics of Energy Source Utilization

Santana¹,

Nascimento²,

Costa³

et al. 2014

Journal of Insect Science

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Energy homeostasis is an essential process during oogenesis, nutrients are required for suitable embryonic development, and recently, studies have investigated metabolic activity during this process. This work aims the investigation of dynamics of energy source utilization of Rhynchophorus palmarum during embryogenesis. For this, we first evaluated the mobilization kinetics of the lipids and glycogen. Thereafter, the synthesis of RNA, protein, and the involvement of enzyme of the glycolytic and pentose-phosphate pathways. Results showed that lipid content decreased in contrast with the lipase activity. The total glycogen amounts it was partly consumed and the glucose content increased, but then values remained stable until hatching. Total RNA content increased, and no significant changes in total protein content were observed. A study of the glycolytic pathway data showed activity of hexokinase and pyruvate kinase at the beginning of embryogenesis. Furthermore, glucose-6-phosphate formed is driven into the pentose-phosphate pathway viewed the high activity of glucose-6-phosphate dehydrogenase. Finally, these results showed that mobilization of different energy sources together with different enzymatic activities has an important role in embryonic development of R. palmarum .

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“…Glycogen content was measured as described by Santos et al (2008) . Briefly, 100 μl supernatant (from section 2.3) was incubated for 4 h at 37°C in the presence of 20 μl (1 U) amyloglucosidase (EC 3.2.1.3; Sigma-Aldrich) diluted in 100 mM sodium acetate (pH 5.5) to hydrolyze glycogen.…”

Section: Methodsmentioning

confidence: 99%

Effects of starvation on the carbohydrate metabolism in Harmonia axyridis (Pallas)

Shi

Wang

et al. 2017

Biology Open

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Trehalose plays an important role in energy storage, metabolism, and protection from extreme environmental conditions in insects. Trehalose is the main blood sugar in insects, and it can be rapidly used as an energy source in times of need. To elucidate the mechanisms of the starvation response, we observed the effects of starvation on trehalose and glycogen, trehalase activity, and the relative gene expression of genes in the trehalose and glycogen metabolic pathways in the invasive beetle Harmonia axyridis. Our results show that trehalose levels and the activities of two types of trehalases decreased significantly in the first 8 h of starvation, while the relative expression of HaTreh1-1 increased. While trehalose remained nearly constant at a relatively high level from 8 to 24 h, glycogen levels decreased significantly from 8 h to 24 h of starvation. Likewise, glycogen phosphorylase (HaGP) expression was significantly higher at 12 to 24 h starvation than the first 8 h, while the expression of glycogen synthase (HaGS) was relatively stable. Furthermore, trehalose decreased significantly from 24 h starvation to 72 h starvation, while trehalase activities and the relative expression of some HaTreh genes generally increased toward the end of the starvation period. The expression of trehalose-6-phosphate synthase (HaTPS) increased significantly, supporting the increase in trehalose synthesis. These results show that trehalose plays a key role in the energy provided during the starvation process through the molecular and biochemical regulation of trehalose and glycogen metabolism.

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Carbohydrate accumulation and utilization by oocytes of Rhodnius prolixus

Cited by 34 publications

References 27 publications

Glycogen Phosphorylase and Glycogen Synthase: Gene Cloning and Expression Analysis Reveal Their Role in Trehalose Metabolism in the Brown Planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae)

Glycogen Phosphorylase and Glycogen Synthase: Gene Cloning and Expression Analysis Reveal Their Role in Trehalose Metabolism in the Brown Planthopper, Nilaparvata lugens Stål (Hemiptera: Delphacidae)

Embryonic Development of Rhynchophorus palmarum (Coleoptera: Curculionidae): Dynamics of Energy Source Utilization

Effects of starvation on the carbohydrate metabolism in Harmonia axyridis (Pallas)

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