Carbohydrate-Bearing Cell Surface Receptors Involved in Innate Immunity: Interleukin-12 Induction by Mitogenic and Nonmitogenic Lectins

Muraille, Eric; Pajak, Bernard; Urbain, Jacques; Léo, Oberdan

doi:10.1006/cimm.1998.1401

Cited by 35 publications

(19 citation statements)

References 37 publications

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“…Here, we demonstrate that ArtinM-induced proliferation of murine spleen cells is independent of TLR2. Most of the lectins assayed by Muraille et al (1999), and now verified for ArtinM, have been shown to stimulate both lymphocyte proliferation and IL-12 production, suggesting that they recognize specific oligosaccharides on the surface of the cells involved in adaptative and innate immunity.…”

Section: Discussionmentioning

confidence: 89%

“…Although mandatory, evaluation of the ArtinM direct effect on cells of the adaptive immunity has been withdrawn. Indeed, correlation between the properties of several plant lectins of stimulating IL-12 production and inducing lymph proliferation has been examined previously and showed that only wheat germ agglutinin of Triticum vulgare induced IL-12 production without promoting cell proliferation or IL-2 release (Muraille et al 1999). Here, we demonstrate that ArtinM-induced proliferation of murine spleen cells is independent of TLR2.…”

Section: Discussionmentioning

confidence: 99%

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Activation of spleen cells by ArtinM may account for its immunomodulatory properties

et al. 2014

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ArtinM is a D-mannose-binding lectin extracted from Artocarpus heterophyllus that promotes interleukin-12 production by macrophages and dendritic cells. This property is considered responsible for T helper 1 immunity induced in vivo after ArtinM administration. In this study, we investigated the effect of native (jArtinM) and recombinant (rArtinM) forms of lectin on murine spleen cells and isolated T lymphocytes. We found that ArtinM binds to the surface of spleen cells. This interaction, which was blocked by D-mannose, induced cell activation, as manifested by increased mitochondrial activity, interleukin-2 production, and cell proliferation. We verified that a 30-times higher concentration of rArtinM was required to trigger optimal activation of spleen cells compared with that needed with jArtinM, although these proteins have identical sugar recognition properties and use the same signaling molecules to trigger cell activation. Because the distinction between native and recombinant is restricted to their tertiary structure (tetrameric and monomeric, respectively), we postulated that the multi-valence of jArtinM accounts for its superiority in promoting clustering of cell surface glycoreceptors and activation. The jArtinM and rArtinM activation effect exerted on spleen cells was reproduced on purified CD4+ T cells. Our results suggest that ArtinM interaction with T cells leads to responses that may act in concert with the interleukin-12 produced by antigen-presenting cells to modulate immunity toward the T helper 1 axis. Further studies are necessary to dissect ArtinM/T-cell interactions to more fully understand the immunomodulation induced by carbohydrate recognition.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Activation of spleen cells by ArtinM may account for its immunomodulatory properties

et al. 2014

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“…Despite the fact that PHA is one of the mitogenic lectins endowed with IL-12-inducing activity [17], the presence of C3bgp showed additive effect on the IL-12 production in PHA stimulating culture. Since IL-12 is not produced by T cells [12][13][14], the increased IL-12 production observed following stimulation with C3bgp might be due to stimulation of monocytes and/ or B cells.…”

Section: Discussionmentioning

confidence: 93%

C3 binding glycoprotein from Cuscuta europea induced different cytokine profiles from human PBMC compared to other plant and bacterial immunomodulators

Stanilova

Dobreva

Slavov

et al. 2005

International Immunopharmacology

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“…14 Some studies have shown the involvement of lectins in cell signaling initiated by TLRs, 14 -16 as well as in the induction or amplification of IL-12 17,18 and IFN-␥ 19,20 production. Our group has previously shown that BALB/c mice prophylactically treated with KM ϩ , a D-mannose-binding lectin from the seeds of Artocarpus integrifolia, develop a Th1 protective response against Leishmania major infection.…”

Section: Kmmentioning

confidence: 99%

Therapeutic Administration of KM+ Lectin Protects Mice Against Paracoccidioides brasiliensis Infection via Interleukin-12 Production in a Toll-Like Receptor 2-Dependent Mechanism

Coltri

Oliveira

Pinzan

et al. 2008

The American Journal of Pathology

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KM؉ is a mannose-binding lectin from Artocarpus integrifolia that induces interleukin (IL)-12 production by macrophages and protective T helper 1 immune response against Leishmania major infection. In this study, we performed experiments to evaluate the therapeutic activity of jackfruit KM ؉ (jfKM ؉ ) and its recombinant counterpart (rKM ؉ ) in experimental paracoccidioidomycosis. To this end, jfKM ؉ or rKM ؉ was administered to BALB/c mice 10 days after infection with Paracoccidiodes brasiliensis. Thirty days postinfection, lungs from the KM ؉ -treated mice contained significantly fewer colony-forming units and little to no organized granulomas compared to the controls. In addition, lung homogenates from the KM ؉ -treated mice presented higher levels of nitric oxide, IL-12, interferon-␥, and tumor necrosis factor-␣, whereas higher levels of IL-4 and IL-10 were detected in the control group. With mice deficient in IL-12, Toll-like receptor (TLR) 2, TLR4, or TLR adaptor molecule MyD88, we demonstrated that KM ؉ led to protection against P. brasiliensis infection through IL-12 production, which was dependent on TLR2. These results demonstrated a beneficial effect of KM ؉ on the severity of P. brasiliensis infection and may expand its potential use as a novel immunotherapeutic molecule.

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Carbohydrate-Bearing Cell Surface Receptors Involved in Innate Immunity: Interleukin-12 Induction by Mitogenic and Nonmitogenic Lectins

Cited by 35 publications

References 37 publications

Activation of spleen cells by ArtinM may account for its immunomodulatory properties

Activation of spleen cells by ArtinM may account for its immunomodulatory properties

C3 binding glycoprotein from Cuscuta europea induced different cytokine profiles from human PBMC compared to other plant and bacterial immunomodulators

Therapeutic Administration of KM+ Lectin Protects Mice Against Paracoccidioides brasiliensis Infection via Interleukin-12 Production in a Toll-Like Receptor 2-Dependent Mechanism

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