Carbohydrate Deficient Transferrin and Interleukin-6 as Predictors of Fibrosis in Alcohol Cirrhosis

Nandeesha, Hanumanthappa; Rajappa, Medha; Kadhiravan, Tamilarasu; Srilatha, Krishnamoorthy; Harichandrakumar, K T; Thyagarajan, Durgadevi

doi:10.1007/s12291-015-0534-9

Cited by 4 publications

(2 citation statements)

References 15 publications

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“…Previous reports have suggested the possibility of using selected interleukins as biochemical markers of cirrhosis. Nandeesha et al suggested that one of the predictors of fibrosis in alcoholic cirrhosis may include elevated interleukin-6 [18]. Yang et al investigated serum levels of FGF-21 in diagnosing nonalcoholic steatoheaptitis (NASH) and the critical stage of non-alcoholic fatty liver disease (NAFLD) [19].…”

Section: Discussionmentioning

confidence: 99%

Oxidative stress-induced growth inhibitor 1 in alcohol-induced liver cirrhosis

Sak¹,

Prystupa²,

Bis-Wencel³

et al. 2021

Ann Agric Environ Med.

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Introduction andObjective. Human oxidative stress-induced growth inhibitor 1 (OSGIN1) is a protein identified in 2001 which belongs to the OKL38 protein family. The aim of the study was to investigate the levels of this protein depending on the severity of alcohol-induced liver cirrhosis. Materials and method. The study group consisted of 60 patients: 30 patients with cirrhosis in the P-Ch A and B stage and 30 in the P-Ch C stage. The control group consisted of 18 healthy individuals without liver diseases, who did not abuse alcohol. Oxidative stress induced growth inhibitor 1 (OSGIN1), fibroblast growth factor 1 (FGF1) and fibroblast growth factor 21 (FGF21) were determined in blood serum using enzyme-linked immunosorbent assay (ELISA) kits. All absorbance readings were conducted using an Epoch Microplate Spectrophotometer (BioTek Instrumentals, Inc., Winooski, VT, USA). OSGIN1, FGF1 and FGF21 concentrations were determined using Sandwich enzyme immunoassay kits (by Cloud Clone Corp., Katy, TX, USA). Statistica 13.3 (TIBCO Software, Inc.) was used for data analysis. Results. The concentration of OSGIN1 was 0.028 ± 0.017 in the control group which increased with the advancement of liver cirrhosis (stage of Pugh-Child): 0.075 ± 0.098 in the P-Ch A + B group and 0.121 ± 0.134 in the P-Ch C stage. Multiple comparison tests confirmed statistically significant differences in OSGIN1 concentration between the control group and P-Ch C (p <0.02). Significant correlations were noted between OSGIN1 and FGF1 (r = 0.39; p = 0.004) and between OSGIN1 and FGF21 (r = 0.53; p <0.0001). Conclusions. The study revealed that the level of OSGIN1 increased significantly in the P-Ch C stage of liver cirrhosis. It is possible that OSGIN1 may be used for the non-invasive diagnosis of ALD, but its possible diagnostic value is still very uncertain.

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Section: Discussionmentioning

confidence: 99%

Oxidative stress-induced growth inhibitor 1 in alcohol-induced liver cirrhosis

Sak¹,

Prystupa²,

Bis-Wencel³

et al. 2021

Ann Agric Environ Med.

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“…A quantitative, sandwich enzyme immunoassay technique was used to measures CDT from serum samples. [19] The assay was performed by ELISA (Enzyme-Linked Immuno-Sorbent Assay) technique (Tecan GENios ELISA reader, Austria GmbH, Austria), using Magellan software. The procedure was followed as per the protocol provided by the manufacturer (Cusabio, USA).…”

Section: Phase 1: Optimizationmentioning

confidence: 99%

Use of Filter Paper to Measure Alcohol Biomarkers among Opioid-Dependent Patients on Agonist Maintenance Treatment: A Community-Based Study

Quraishi

Varshney

Singh

et al. 2019

Indian Journal of Psychological Medicine

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Background:Harmful Alcohol use is frequent among opioid dependents patients undergoing agonist maintenance treatment. The objective assessment of harmful alcohol use can be done using laboratory measures of serum biomarkers. For community-based patients, there is often a requirement of an alternative method due to lack of onsite laboratory services. The aim of the study was to examine filter paper as a matrix to measure serum biomarkers of harmful alcohol use.Methods:The initial phase involved standardization of the filter-paper-based assay. Conditions were optimised for extraction and estimation of alcohol biomarkers (Aspartate Aminotransferase; AST, Alanine Aminotransferase; ALT, Gamma Glutamyl transferase; GGT and Carbohydrate Deficient Transferrin; CDT) from the filter paper. For clinical validation, serum samples were collected from community clinics. Biomarker levels obtained from both the methods were correlated using linear regression analysis. Limits of agreement between the two methods was estimated using the Intraclass Correlation Coefficient (ICC).Results:The extraction of enzymes (AST, ALT and GGT) from filter paper was carried out using the substrate buffer available with the reagent kit (Randox, UK). CDT was readily extracted from filter paper using deionised water. Serum biomarker levels measured from samples collected from community clinics correlated well with filter paper extracted levels (ICC 0.97-0.99). More than 90% of alcohol biomarker levels were recovered from the filter paper matrix using this method.Conclusion:Filter paper has the potential to be used as a matrix to objectively measure alcohol biomarkers among opioid-dependent patients from community settings lacking onsite laboratory facilities.

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