Carbohydrate fermentation and by-product absorption studied with labelled cellulose in Oryctes nasicornis larvae (Coleoptera:Scarabaeidae)

Bayon, Colette; Mathelin, Jocelyne

doi:10.1016/0022-1910(80)90100-6

Cited by 53 publications

(40 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, our in vitro digestion studies showed that > 90% of the labelled digestion products were organic or amino acids (Table 5), presumably derived from microbial metabolism of the labelled substrate. In similar experiments using radiolabelled cellulose and ligated gut segments from the terrestrial beetle Oryctes nasicornis, Bayon and Mathelin (1980) reported an absence of labelled hexose sugars in both gut milieu and incubation media, but found a variety of labelled organic acids. They attributed these findings to a rapid conversion of sugars liberated from the labelled cellulose by gut endosymbionts.…”

Section: Discussionmentioning

confidence: 95%

“…Finally, our in vitro digestion experiments showed that a significant fraction (40%) of the radiolabelled digestive products crossed the gut wall as neutral sugar. We would not expect to find significant amounts of free sugar either in the gut or passing the gut wall were endosymbionts a major pathway available for cellulolysis (Bayon and Mathelin 1980). But with 60% of the label crossing the gut wall as organic and amino acids, some microbial metabolism of liberated sugars obviously occurred.…”

Section: Discussionmentioning

confidence: 99%

“…Second, the near absence of detectable cellulase activity in the guts of tipulid larvae is consistent with an endosymbiotic mode of cellulolysis. As pointed out by Bayon and Mathelin (1980), bacterial cellulases tend to remain tightly bound to the cell walls and do not enter the gut milieu. Such enzymes would not be detected by normal enzyme assays of the sorts employed here.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Cellulose Digestion and Assimilation by Three Leaf‐shredding Aquatic Insects

Sinsabaugh¹,

Linkins²,

Benfield³

1985

Ecology

View full text Add to dashboard Cite

JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact support@jstor.org.. Abstract. The capacity of three leaf-shredding aquatic insects, Pteronarcys proteus (Plecoptera: Pteronarcidae), Tipula abdominalis (Diptera: Tipulidae), and Pycnopsyche luculenta (Trichoptera: Limnephilidae), to digest and assimilate cellulose was investigated. Pteronarcys nymphs collected from two second-order woodland streams over a 14-mo period exhibited high levels of cellulolytic activity in their alimentary tracts, especially in the anterior gut. Similar though slightly lower activity levels were measured in Pycnopsyche guts. Cellulolytic activity in Tipula larvae collected from the streams during the same period was low to absent, and when present, was concentrated in the hindgut. General proteolytic activity was similar in the alimentary tracts of all three species. Ecological Society of AmericaAssimilation of uniformly labelled 14C-cellulose was determined by a dual-label technique, and assimilation efficiencies were estimated at 11.2% for Pteronarcys, 18.5% for Tipula, and 12.0% for Pycnopsyche. Confirmation that labelled digestion products passed the gut wall in two species was obtained by in vitro label transport experiments. Ion exchange fractionation of labelled digestion products crossing the gut wall showed that >90% of the label was transported as organic acids and amino acids in Tipula, while >40% of the label crossing the gut wall in Pteronarcys was neutral sugar. Based on the label experiments and published information, we hypothesize that Tipula relies mainly on microbial endosymbionts for cellulose hydrolysis, while Pteronarcys accomplishes hydrolysis largely by means of acquired microbial enzymes obtained through ingestion of microbially conditioned detritus. This study demonstrates the potential for certain leaf-shredding stream insects to derive nutritional benefit from plant polysaccharides, although not without microbial mediation.

show abstract

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Cellulose Digestion and Assimilation by Three Leaf‐shredding Aquatic Insects

Sinsabaugh¹,

Linkins²,

Benfield³

1985

Ecology

View full text Add to dashboard Cite

show abstract

“…The highest pH values among beetle larvae were encountered among the Scarabaeidae (50,52,58,60), which comprise species from humivorous, detritivorous, and coprophagous feeding guilds. Although scarab beetle larvae are among those few arthropods that have received at least a minimum of attention from a microbiological perspective (3,4,21,23) and although their importance for the transformation of soil organic matter is undisputed, little is known about the composition of the gut microbial community and its role in the digestive process.Using the humivorous larva of the cetoniid beetle Pachnoda ephippiata (Coleoptera: Scarabaeidae) as a model organism, we investigated the physicochemical conditions and microbial activities in the gut in order to gain more insight into the role of the intestinal microbiota in transformation and mineralization of organic matter during gut passage. Since a representative analysis of the microbial community structure requires covering also those populations that resist cultivation (14), the project also included a cultivation-independent approach, involving molecular cloning and fingerprinting techniques.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Physicochemical Conditions and Microbial Activities in the Highly Alkaline Gut of the Humus-Feeding Larva of Pachnoda ephippiata (Coleoptera: Scarabaeidae)

Lemke

Stingl

Egert

et al. 2003

Appl Environ Microbiol

159

143

View full text Add to dashboard Cite

The soil macrofauna plays an important role in the carbon and nitrogen cycle of terrestrial ecosystems. In order to gain more insight into the role of the intestinal microbiota in transformation and mineralization of organic matter during gut passage, we characterized the physicochemical conditions, microbial activities, and community structure in the gut of our model organism, the humus-feeding larva of the cetoniid beetle Pachnoda ephippiata. Microsensor measurements revealed an extreme alkalinity in the midgut, with highest values (pH > 10) between the second and third crown of midgut ceca. Both midgut and hindgut were largely anoxic, but despite the high pH, the redox potential of the midgut content was surprisingly high even in the largest instar. However, reducing conditions prevailed in the hindgut paunch of all instars (E h ϳ ؊100 mV). Both gut compartments possessed a pronounced gut microbiota, with highest numbers in the hindgut, and microbial fermentation products were present in high concentrations The soil macrofauna plays an important role in the carbon and nitrogen cycle of terrestrial ecosystems (18,41,62). The intestinal tracts of litter-feeding and humivorous soil macroinvertebrates are favorable habitats for microorganisms and typically harbor a dense and active gut microbiota. The major function commonly attributed to the microorganisms in the guts of such animals is the depolymerization and fermentative breakdown of the cellulosic or lignocellulosic component of their diet, which leads to degradation products that can be resorbed by the host. This is supported by the high concentrations of microbial fermentation products and by the presence of fermentative bacteria and protozoa, accompanied by obligately anaerobic homoacetogenic and methanogenic microorganisms, in the guts of such animals. However, the extent and importance of such processes and their specific function in the nutrition of the host are scarcely understood (for reviews, see references 8, 9, 11, 12, 14, 36, and 44).In the case of soil-feeding termites, host factors such as the extreme alkalinity of the anterior hindgut and the influx of oxygen seem to play a key role in sequestering organic matter from the inorganic soil matrix (37). The decrease of molecular weight and increase in solubility resulting from alkaline extraction and chemical oxidation render the organic matter accessible for digestion in subsequent, less-alkaline compartments (34, 35). Alkaline gut regions are encountered also in many representatives of other insect orders and seem to be connected with the dietary preferences of the respective taxa (Coleoptera, Diptera, and Lepidoptera; see references 15 and 32). Comparing several beetle larvae feeding on a lignocellulosic diet, Grayson (30) already had pointed out an apparent correlation between the degree of humification of the diet and the alkalinity of the intestinal tract. The highest pH values among beetle larvae were encountered among the Scarabaeidae (50,52,58,60), which comprise species from humivorous, ...

show abstract

Characterization of cellulose degrading bacteria from the larval gut of the white grub beetle Lepidiota mansueta (Coleoptera: Scarabaeidae)

Handique

Phukan

Bhattacharyya

et al. 2017

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

The goal of this study is to identify and characterize the cellulose degrading microorganisms in the larval gut of the white grub beetle, Lepidiota mansueta. Thirty bacterial strains were isolated and tested for cellulolytic activity using soluble carboxymethyl cellulose (CMC) degrading assays. Of these strains, five (FGB1, FB2, MB1, MB2, and HB1) degrade cellulose. Cellulolytic activity was determined based on formation of clear zone and cellulolytic index on CMC plate media. The highest cellulolytic index (2.14) was found in FGB1. Partial 16S rDNA sequencing, morphological, and biochemical tests were used to identify and characterize the five isolates, all Citrobacter sp. (Enterobacteriaceae). This study identifies new cellulose degrading microorganisms from the larval gut of L. mansueta. The significance of identifying these strains lies in possible application in cellulose degradation.

show abstract

Carbohydrate fermentation and by-product absorption studied with labelled cellulose in Oryctes nasicornis larvae (Coleoptera:Scarabaeidae)

Cited by 53 publications

References 13 publications

Cellulose Digestion and Assimilation by Three Leaf‐shredding Aquatic Insects

Cellulose Digestion and Assimilation by Three Leaf‐shredding Aquatic Insects

Physicochemical Conditions and Microbial Activities in the Highly Alkaline Gut of the Humus-Feeding Larva of Pachnoda ephippiata (Coleoptera: Scarabaeidae)

Characterization of cellulose degrading bacteria from the larval gut of the white grub beetle Lepidiota mansueta (Coleoptera: Scarabaeidae)

Contact Info

Product

Resources

About