Carbohydrate fermentation plate medium for confirmation of Neisseria species

a rapid enzymatic method with chromogenic substrates, was tested in our laboratories for the identification of Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica. The test correlated very highly in its identification of pathogenic Neisseria spp. with modified New York City fermentation medium. Identicult-Neisseria appeared to be more sensitive in its detection of prolylaminopeptidase activity in N. meningitidis than most of the currently available systems.

mentioning

confidence: 99%

Increased detection of prolylaminopeptidase in Neisseria meningitidis by Identicult-Neisseria

Sperry¹,

Cohenford²,

Campognone³

et al. 1986

“…It is evident from the number of recent publications suggesting modifications for improving the reliability of the sugar degradation tests that they still pose a significant problem for many diagnostic laboratories (2,4,10,12,17,20,21). In several comparative studies by different workers to determine the most reliable of the various types of sugar utilization tests, the numbers of positives obtained, even when the same type of test was used, varied as much as 30 to 35% (3,14,16).…”

mentioning

confidence: 99%

Lectins in diagnostic microbiology: use of wheat germ agglutinin for laboratory identification of Neisseria gonorrhoeae

Schaefer¹,

Keller²,

Doyle³

1979

A lectin slide agglutination test has been developed for the confirmatory identification of Neisseria gonorrhoeae. With wheat germ lectin as an agglutinin, 164 of 165 clinical isolates of N. gonorrhoeae gave a 3 to 4+ reaction within 6 to 8 min. Four gonococcal isolates, even though negative by the fluorescent-antibody method, gave strong positive reactions with the wheat germ lectin. Among 23 isolates of Neisseria meningitidis tested, which included representatives of serogroups A, B, C, D, X, Y, and Z, only one strain in group X gave a false-positive reaction. The nonpathogenic species of Neisseria, as well as Branhamella catarrhalis, all showed negative reactions with the wheat germ agglutinin. The novel method provides a simple, rapid, and inexpensive means for the laboratory diagnosis of gonorrhea and obviates the need for performing second-stage sugar fermentation studies or utilizing the more expensive fluorescent-antibody techniques.

“…The most commonly used method is carbohydrate degradation reactions in CTA. The CTA medium not only requires 24 to 48 h of incubation, but also has been found to be inadequate in certain cases owing to the failure of occasional strains of gonococci to produce acid from glucose (6,11,16,19,25). Consequently, numerous methods have been reported in the literature which are claimed to be both sensitive and rapid (5,11,12,16,19,24,26).…”

Section: Resultsmentioning

confidence: 99%

Clinical evaluation of the rapid carbohydrate degradation microtube method for identification of Neisseria species

Qadri¹,

Parker²

1981

The rapid carbohydrate degradation (Carr Microbiologicals Wichita, Kans.) microtube method is a new test system designed for the identification of Neisseria spp. The system consists of four microtubes containing different carbohydrates in a peptone-Bitone basal medium. This method was evaluated for accuracy and speed in identifying species of Neisseria. Of the 386 clinical isolates used in this study, 98.4% were correctly identified to species level in 4 h with the rapid carbohydrate degradation system; parallel testing of the same isolates with conventional cystine-tryptic agar resulted in 96.1% accuracy in 48 h.