Carbohydrate metabolism in Nerine sarniensis bulbs developing in liquid culture

Vishnevetsky, Jane; Zamski, E.; Ziv, Meira

doi:10.1034/j.1399-3054.2000.108004361.x

Cited by 21 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The high values of starch compared with the generally low values of the soluble sugars of the hyacinth scales and the gradual reduction and disappearance of sugars from 4 to 7 months after planting as well as the accumulation of starch in the scales from 5 to 7 month after planting imply that starch was the major storage carbohydrate in hyacinth. Vishnevetsky et al (2000) also worked on Nerine sarniensis cv Salmon and reported that starch was the dominant storage carbohydrate in that bulb.…”

Section: Discussionmentioning

confidence: 99%

“…Thus for example, during the early shoot growth of most geophytes, when stored reserves are utilized, starch content of the storage organs is expected to decrease, and subsequently increase after anthesis, because at this point, carbohydrate filling is rapid (Miller, 1992). Vishnevetsky et al (2000) reported that, only small changes in starch content were detected during the first month of bulb growth, but an increase in starch level was observed at later stages of development. They observed that the activity of ADP-glucose pyrophosphorylase, a key enzyme of starch synthesis, increased just before the increase in starch accumulation, and sucrose was the dominant soluble sugar in the bulbs whilst only traces of glucose and fructose were detected.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Regulation of carbohydrates partitioning and metabolism of the common hyacinth

Addai¹,

Scott²

2011

ABJNA

View full text Add to dashboard Cite

The biochemical principles underlining the partitioning and metabolism of starch in most ornamental geophytes are poorly understood. Studies were undertaken at the Biology and Environmental Science Department of the University of Sussex, Falmer, UK with a view to investigating the regulation and partitioning of carbohydrates in flower bulbs using the common hyacinth as test plant. Results indicated that, among the various carbohydrates measured in this geophyte, starch was the major storage carbohydrate, and its degradation occurred through amylolysis rather than phosphorolysis. Starch and biomass contents of the bulb scales decreased whilst those of the newly formed organs increased after planting. Following the depletion of the reserves in the bulb scales, the flower accumulated the highest amount of starch and biomass whilst the stem and roots had the least amount of these quantities. The distribution of enzymes involved in starch degradation in the bulb scales closely followed the pattern exhibited by starch degradation in these organs. Similarly, starch and biomass accumulation of the newly formed organs mimicked the accumulation of these quantities in those organs. ADP glucose pyrophosphorylase and starch synthase were equally responsible for the accumulation of starch in the flower as well as the newly formed leaves, but these enzymes were less responsible for starch accumulation in the stem and roots.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Regulation of carbohydrates partitioning and metabolism of the common hyacinth

Addai¹,

Scott²

2011

ABJNA

View full text Add to dashboard Cite

show abstract

“…Various carbohydrates are used for these purposes, such as sucrose, glucose, and fructose. When used in tissue cultures, sucrose is hydrolyzed to glucose and fructose, which are utilized for plant growth (Vishnevetsky et al 2000;George et al 2008). Sucrose content was decreased to 0.5% in all treatments after two weeks, and therefore it is necessary to supply sucrose with a salt compound for longterm cultures.…”

Section: Discussionmentioning

confidence: 99%

Effects ofin vitroculture types on regeneration and acclimatization of yellow poplar (Liriodendron tulipiferaL.) from somatic embryos

An¹,

Kim²,

Moon³

et al. 2016

J Plant Biotechnol

View full text Add to dashboard Cite

We compared germination efficiency for somatic embryos (SE) of Liriodendron tulipifera using semi-solid (SS), temporary immersion bioreactors (TIB), and continuous immersion bioreactors (CIB) to produce vigorous plants. The bioreactors were designed to be immersed in liquid media with plantlets with an adjustable immersion time. TIB and CIB improved germination rates up to 80.86% and 95.21%, respectively, however, CIB produced more hyperhydric plantlets than TIB. The height of plantlets in TIB was significantly higher than for those in CIB. Fresh weights of plantlets grown in CIB of were significantly lower than for those grown in TIB. The lowest chlorophyll concentration was found in in vitro plantlets from CIB. We examined abnormally developed leaves, stems, and apical zones of in vitro plantlets that were produced in CIB. Among the three types, SS showed the highest stomatal density and the shortest stomatal length in in vitro plantlets. After acclimatization, plants from CIB exhibited the lowest values in biomass, such as height, root collar diameter, leaf fresh weight, leaf length, leaf width, petiole length, petiole diameter, and leaf area. Photosynthesis and transpiration rates of ex vitro plants were not significantly different among the three culture types, but stomatal conductance was higher in TIB than in the SS and CIB. Therefore, the results suggest that TIB is the preferable bioreactor to improve in vitro plantlet regeneration of L. tulipifera. TIB-originated plants showed higher growth rate than SS and CIB after transferring to soil.

show abstract

“…Previous studies suggest that development of storage roots was paralleled with complex physiological activities including carbohydrate, enzyme, and hormone dynamics [2][3][4]. A wealth of studies regarding this have been conducted on bulbous owers such as lilies [5][6][7] and tulips [2,3,8], whereas freesia remains unexplored according to our knowledge.…”

Section: Introductionmentioning

confidence: 99%

“…Miao et al [2,3] revealed that the development of Tulipa edulis stolons was accompanied with a sugar consumption and starch production via the action of enzymes. Vishnevetsky et al [4] found that content of carbohydrates were strongly correlated to corresponding enzyme activities in Nerine sarniensis bulbs at different developmental stages. The signi cance of metabolic enzymes in carbohydrate metabolism in developmental bulbs was further revealed in oriental hybrid lily 'Sorbonne' [16] and Gladiolus hybridus [17].…”

Section: Introductionmentioning

confidence: 99%

Dynamics and Transcriptome Analysis of Starch and Sucrose Metabolism During Corm Development in Freesia Hybrida

Su-Qin

Yan

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Starch and sucrose metabolism plays a crucial role in the formation and development of bulbs in bulbous plants, which, however, remains unclear and unexplored in the corms of Freesia hybrida, one kind of famous bulbous flower. Herein, we investigated the dynamics of the major form of carbohydrates and related enzyme activities and profiled the transcriptome of freesia corms at four developmental stages with the aim to reveal the relation of starch and sucrose metabolism to corm development and the transcriptional regulation of this metabolic process.Results The content of starch, sucrose and soluble sugars followed an overall upward trend across the corm developmental stages. Activities of the adenosine diphosphoglucose pyrophosphorylase, starch branching enzyme and β-amylase generally followed the pattern of the starch and sucrose level. Activities of sucrose phosphate synthase increased from corm formation till the initial swelling stage and subsequently reached a plateau. Activities of invertase and sucrose synthase peaked at the later rapid swelling stage. Transcriptome analysis revealed a total of 100,999 unigenes, out of which 44,405 unigenes were annotated. Analysis based on Clusters of Orthologous Groups suggested that carbohydrate transport and metabolism (9.34% of the sequences) was prominent across the corm developmental process. Totally 3427 differentially expressed genes were identified and the enrichment analysis directed starch and sucrose metabolism a critical pathway in corm development especially at the rapid swelling stage. Genes encoding key carbohydrate-metabolizing enzymes were identified and their differential expression related to corm development was explored.Conclusions The comparative transcriptome analysis discloses essential roles of starch and sucrose metabolism and the genetic mechanism related to the corm development. The results construct a valuable resource pool for further molecular-level studies, which are helpful for metabolic regulation of carbohydrates and improvement in molecular breeding of Freesia hybrida.

show abstract

Carbohydrate metabolism in Nerine sarniensis bulbs developing in liquid culture

Cited by 21 publications

References 0 publications

Regulation of carbohydrates partitioning and metabolism of the common hyacinth

Regulation of carbohydrates partitioning and metabolism of the common hyacinth

Effects ofin vitroculture types on regeneration and acclimatization of yellow poplar (Liriodendron tulipiferaL.) from somatic embryos

Dynamics and Transcriptome Analysis of Starch and Sucrose Metabolism During Corm Development in Freesia Hybrida

Contact Info

Product

Resources

About