Carbohydrate Metabolite Pathways and Antibiotic Production Variations of a Novel Streptomyces sp. M3004 Depending on the Concentrations of Carbon Sources

Abstract

To determine the variations of growth, some key enzyme activities such as glucose kinase (GK), glucose-6-phosphate dehydrogenase (G6PDH), α-ketoglutarate dehydrogenase (KGDH), and isocitrate lyase (ICL) besides metabolite levels of pyruvate and antibiotic production of newly isolated Streptomyces sp. M3004 were grown in culture media which contain 10-20 g/l concentration with either glucose or glycerol as carbon source. Biomass and intracellular glucose and glycerol levels of Streptomyces sp. M3004 showed positive correlation with the concentration of these carbon sources, and these levels were higher in glucose compared with the glycerol-supplemented mediums. GK, G6PDH, and KGDH activities showed marked correlation with the concentration of both glucose and glycerol, and the activity levels were 4.14-, 1.47-, and 1.27-fold higher in glucose than glycerol. A key enzyme of the glyoxalate cycle, ICL activities decreased with increasing glucose concentrations from 10 to 20 g/l, but increased up to 15 g/l of glycerol. The positive correlations were also determined between intracellular glucose and glycerol levels besides pyruvate and protein variations with respect to concentrations of the carbon sources. Antibacterial activities of Streptomyces sp. M3004 reached maximum on the stationary phase, while it did not change significantly with respect to glucose and glycerol.

Comparative Transcriptomics Analysis of the Responses of the Filamentous Fungus Glarea lozoyensis to Different Carbon Sources

“…PB 0 , the precursor of the antifungal drug caspofungin, is a secondary metabolite of the fungus G. lozoyensis (Bills et al, 1999;Youssar et al, 2012), and its biosynthesis is affected by the types and concentrations of nutrients in the medium. Among them, the carbon source is a key factor affecting cell growth and PB 0 production (Sanchez and Demain, 2002;Kayali et al, 2011). However, recent research on G. lozoyensis has mainly focused on biosynthetic gene clusters and fermentation regulation strategies (Chen et al, 2013(Chen et al, , 2015Song et al, 2018a,c), while the mechanisms that regulate the carbon source metabolism are still unknown.…”

“…During the culture process, nutrient components and metabolic compounds in the medium have a great influence on the synthesis of SMs (Sanchez and Demain, 2002). Among them, the carbon source is the most important influencing factor, and optimization of carbon sources has been the subject of continuous research (Kayali et al, 2011). Tkacz et al (1993) found that using 100 g/L mannitol as the sole carbon source improved the yield of PB 0 to 800 mg/L.…”

Antimicrobial effect of black pepper petroleum ether extract for the morphology of Listeria monocytogenes and Salmonella typhimurium

“…And the cell debris has been removed through centrifugation at 10,000 rpm per 10 min at 4°C. The supernatants were collected and the protein content of the mixture was examined by xylene brilliant cyanine G250 (Kayali et al 2011). In addition, Na ?…”

Antibacterial Effect of Black Pepper Petroleum Ether Extract against Listeria monocytogenes and Salmonella typhimurium

“…e activities of POD and CAT were explored by a POD assay kit and CAT assay kit, respectively. e content of the protein was determined by the Bradford method at 595 nm, and the standard curve was made using bovine serum albumin [22].…”