Carbohydrate–Neuroactive Hybrid Strategy for Metabolic Glycan Engineering of the Central Nervous System <i>in Vivo</i>

Shajahan, Asif; Parashar, Shubham; Goswami, Surbhi; Ahmed, Syed Meheboob; Nagarajan, Priyadharsini; Sampathkumar, Srinivasa-Gopalan

doi:10.1021/jacs.6b08894

Cited by 26 publications

(22 citation statements)

References 55 publications

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“…Similarly, incorporation of azido‐sugars was significantly increased during cardiac hypertrophy . Dysregulation of sialoglycan biosynthesis is also found in neurological disorders and central nervous system injuries . These recent findings inspire novel applications in cell‐based therapies and tissue engineering with innate pathophysiological‐responsive monitoring.…”

Section: Cell‐rich Assembliesmentioning

confidence: 85%

Advanced Bottom‐Up Engineering of Living Architectures

et al. 2019

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Bottom‐up tissue engineering is a promising approach for designing modular biomimetic structures that aim to recapitulate the intricate hierarchy and biofunctionality of native human tissues. In recent years, this field has seen exciting progress driven by an increasing knowledge of biological systems and their rational deconstruction into key core components. Relevant advances in the bottom‐up assembly of unitary living blocks toward the creation of higher order bioarchitectures based on multicellular‐rich structures or multicomponent cell–biomaterial synergies are described. An up‐to‐date critical overview of long‐term existing and rapidly emerging technologies for integrative bottom‐up tissue engineering is provided, including discussion of their practical challenges and required advances. It is envisioned that a combination of cell–biomaterial constructs with bioadaptable features and biospecific 3D designs will contribute to the development of more robust and functional humanized tissues for therapies and disease models, as well as tools for fundamental biological studies.

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Section: Cell‐rich Assembliesmentioning

confidence: 85%

Advanced Bottom‐Up Engineering of Living Architectures

et al. 2019

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“…This method uses endogenous metabolic tracers that consist of sialic acid analogs or N-acetylmannosamine (ManNAc) linked with chemical reporters such as azide. In vivo brain imaging of these glycans manipulation techniques has been reported recently ( Gagiannis et al, 2007 ; Sampathkumar et al, 2008 ; Xie et al, 2016 ; Shajahan et al, 2017 ). However, it is difficult to monitor the sialoglycans in the brain tissues because of the labeled glycan’s impermeability through the blood–brain barrier (BBB).…”

Section: Imaging Techniques For the Brain Glycomementioning

confidence: 99%

Mass Spectrometry Imaging for Glycome in the Brain

et al. 2021

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Glycans are diverse structured biomolecules that play crucial roles in various biological processes. Glycosylation, an enzymatic system through which various glycans are bound to proteins and lipids, is the most common and functionally crucial post-translational modification process. It is known to be associated with brain development, signal transduction, molecular trafficking, neurodegenerative disorders, psychopathologies, and brain cancers. Glycans in glycoproteins and glycolipids expressed in brain cells are involved in neuronal development, biological processes, and central nervous system maintenance. The composition and expression of glycans are known to change during those physiological processes. Therefore, imaging of glycans and the glycoconjugates in the brain regions has become a “hot” topic nowadays. Imaging techniques using lectins, antibodies, and chemical reporters are traditionally used for glycan detection. However, those techniques offer limited glycome detection. Mass spectrometry imaging (MSI) is an evolving field that combines mass spectrometry with histology allowing spatial and label-free visualization of molecules in the brain. In the last decades, several studies have employed MSI for glycome imaging in brain tissues. The current state of MSI uses on-tissue enzymatic digestion or chemical reaction to facilitate successful glycome imaging. Here, we reviewed the available literature that applied MSI techniques for glycome visualization and characterization in the brain. We also described the general methodologies for glycome MSI and discussed its potential use in the three-dimensional MSI in the brain.

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“…To this aim, new unnatural monosaccharides have been designed to use specific metabolite transporters through the blood–brain barrier. 71 ManNAz analogs have been synthesized by linking at position 1 or 6 either nicotinic acid, valproic acid, or theophylline-7-acetic acid (mimic of caffeine). These metabolites are vectorized to the brain respectively by nicotinate receptors, medium-chain fatty acids transporters, and adenine transporters.…”

Section: Addressing the Reporter To The Right Placementioning

confidence: 99%