Carbohydrate-rich compounds in the colonic mucosa of man. III. A preliminary report of some biochemical characteristics of the carbohydrate-rich components in normal colonic mucosa

Lk, Korhonen; Mäkelä,

doi:10.1007/bf01003055

Cited by 4 publications

(1 citation statement)

References 14 publications

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“…Chemical analysis shows the presence of hexosamines and sialic acid in human large intestinal mucosa, in agreement with our previous histochemical and autoradiographic findings (Filipe, 1969;Filipe and Cooke, 1970;Filipe, 1971a and b) and other workers' biochemical studies in human (Sky-Peck, Lundgren, and Bornstein, 1966;Korhonen and Makela, 1971), pig (Werner, 1953) and sheep (Pastemak and Kent, 1958;Draper and Kent, 1963) colonic mucosa. Transitional mucosa shows an absolute increase in both total hexosamines and sialic acid.…”

Section: Discussionsupporting

confidence: 91%

Changes in composition of mucin in the mucosa adjacent to carcinoma of the colon as compared with the normal: A biochemical investigation

Filipe¹,

Cooke²

1974

J Clin Pathol

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Fifteen surgical specimens from patients with carcinoma of the colon and rectum were studied. Scrapings from normal mucosa distant from the tumour and from macroscopically normal mucosa adjacent to the tumour (`transitional') were used for chemical estimation of hexosamines, sialic acid, and proteins. The presence of hexosamines and sialic acid was confirmed in both normal and transitional mucosa. Transitional mucosa showed increased levels of total hexosamines and sialic acid as compared with the normal and this was accompanied by an increase in neuraminidase-sensitive sialic acids. The present data have been compared with previous histochemical and autoradiographic studies and it is suggested that the changes described in the transitional mucosa are transformations representing an early stage of carcinogenesis.

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Section: Discussionsupporting

confidence: 91%

Changes in composition of mucin in the mucosa adjacent to carcinoma of the colon as compared with the normal: A biochemical investigation

Filipe¹,

Cooke²

1974

J Clin Pathol

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Histochemical and radioautographic studies of normal human fetal colon

Lev

Orlic

1974

Histochemistry

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Chemical and histochemical studies of normal and diseased human gastrointestinal tract. I. A comparison between histologically normal colon, colonic tumours, ulcerative colitis and diverticular disease of the colon

et al. 1984

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Chemical and histochemical methods were used to compare the epithelial glycoproteins from formalin-fixed surgical specimens of normal human large intestine, colonic tumours, ulcerative colitis and diverticular disease. All the epithelial glycoproteins contained fucose, galactose, glucosamine, galactosamine and, in addition, sialic acids both with and without O-acyl substituents in the side chain and/or at position C4. The glycoproteins of the normal ascending and descending colons differed significantly with respect to the percentage of the sialic acids released following digestion of the de-O-acylated glycoprotein with Vibrio cholera neuraminidase and to the molar fucose-sialic acid ratio. Statistical analysis of the chemical data showed that (a) compared to normal, the sialic acids of the tumour and ulcerative colitis glycoproteins from the descending colon were significantly less substituted in the side chain and at position C4; (b) the O-acetyl substitution pattern of the sialic acids of the ulcerative colitis glycoproteins from the ascending colon and the quantitative composition of the carbohydrate prosthetic groups of the ulcerative colitis glycoproteins from both ascending and descending colons differed from normal; (c) it was not always possible to distinguish between the ulcerative colitis and tumour glycoproteins on the basis of the O-acetyl substitution pattern of their sialic acids; and (d), there were minor differences between normal glycoproteins and those from cases of diverticular disease.

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Carbohydrate-rich compounds in the colonic mucosa of man. III. A preliminary report of some biochemical characteristics of the carbohydrate-rich components in normal colonic mucosa

Cited by 4 publications

References 14 publications

Changes in composition of mucin in the mucosa adjacent to carcinoma of the colon as compared with the normal: A biochemical investigation

Changes in composition of mucin in the mucosa adjacent to carcinoma of the colon as compared with the normal: A biochemical investigation

Histochemical and radioautographic studies of normal human fetal colon

Chemical and histochemical studies of normal and diseased human gastrointestinal tract. I. A comparison between histologically normal colon, colonic tumours, ulcerative colitis and diverticular disease of the colon

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