Carbon black-induced detrimental effect on osteoblasts at low concentrations: Remarkably compromised differentiation without significant cytotoxicity

Ren, Quanzhong; Wu, Yunqin; Ma, Juan; Shan, Qiuli; Liu, Sijin; Liu, Yajun

doi:10.1016/j.ecoenv.2019.04.029

Cited by 15 publications

(13 citation statements)

References 46 publications

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“…Additionally, the functionality of macrophages was assessed post-B( a )P-bearing FBP treatment. Consistent with our recent studies on the proinflammatory reactions upon carbonaceous particles in macrophages, , our pristine FBPs, namely FBPs-B( a )P0, largely provoked the proinflammatory reactions, as reflected by the induction of interleukin 6 (IL-6) (Figure C, P < 0.001). However, compromised production of IL-6 cytokine was found in cells upon FBPs-B( a )P10 and FBPs-B( a )P100, especially the latter, relative to FBPs-B( a )P0, at both 50 μg/mL and 100 μg/mL (Figure C, P < 0.05), indicating blunted proinflammatory responses in FBPs-B( a )P-treated cells.…”

Section: Resultssupporting

confidence: 91%

“…After washing with PBS for 3 times thoroughly to clean the unabsorbed DCF-DA probe, the intensity of the DCF fluorescence was thereafter recorded at 525 nm on a plate reader (Thermo Fisher Scientific Inc., USA) using the excitation wavelength of 488 nm at the corresponding time points, as described. 76 Characterization of the Phagosome and Autophagosome. The phagosomes were examined by TEM.…”

Section: Methodsmentioning

confidence: 99%

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Binding of Benzo[a]pyrene Alters the Bioreactivity of Fine Biochar Particles toward Macrophages Leading to Deregulated Macrophagic Defense and Autophagy

Liu

Yang

et al. 2021

ACS Nano

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Contaminant-bearing fine biochar particles (FBPs) may exert significantly different toxicity profiles from their contaminant-free counterparts. While the role of FBPs in promoting contaminant uptake has been recognized, it is unclear whether the binding of contaminants can modify the biochemical reactivity and toxicological profiles of FBPs. Here, we show that binding of benzo[a]pyrene (B(a)P, a model polycyclic aromatic hydrocarbon) at environmentally relevant exposure concentrations markedly alters the cytotoxicity of FBPs to macrophages, an important line of innate immune defense against airborne particulate matters (PMs). Specifically, B(a)P-bearing FBPs elicit more severe disruption of the phospholipid membrane, endocytosis, oxidative stress, autophagy, and compromised innate immune defense, as evidenced by blunted proinflammatory effects, compared with B(a)P-free FBPs. Notably, the altered cytotoxicity cannot be attributed to the dissolution of B(a)P from the B(a)P-bearing FBPs, but appears to be related to B(a)P adsorption-induced changes of FBPs bioreactivity toward macrophages. Our findings highlight the significance of environmental chemical transformation in altering the bioreactivity and toxicity of PMs and call for further studies on other types of carbonaceous nanoparticles and additional exposure scenarios.

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Section: Resultssupporting

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Binding of Benzo[a]pyrene Alters the Bioreactivity of Fine Biochar Particles toward Macrophages Leading to Deregulated Macrophagic Defense and Autophagy

Liu

Yang

et al. 2021

ACS Nano

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“…CBs signi cantly affected cell proliferation at high concentration (200 µg/L), inhibiting growth more than 20% (Fig. A and B), thereby suggesting the exposure concentration of CBs was lethal doses, as demonstrated previously (Ren et al 2019). There was no signi cant difference apoptosis cell ratio between SB4A and Printex U at the same exposure concentration.…”

Section: Screening and Ascertaining The Combined Exposure Concentration Of Cadmium And Black Carbonsupporting

confidence: 80%

Low-dose Exposure to Black Carbon and Cadmium Significantly Induce Lung Injury by Promoting Cellular Apoptosis

Wang¹,

Bao²,

Liu³

et al. 2021

Preprint

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PMs has adverse biological effects on major living organs in the body, including lungs. The complex composition of PM2.5, including carbon black and heavy metals, cause toxic effects to the lung. The synergistic effects of CB and Cd were less investigated in previous study. In our research, we deciphered the combination of CBs and Cd enhanced the cytotoxicity in BEAS-2B cells in vitro and in vivo. In the molecular level, the intracellular level of Cd increased, as a result of the cell membrane damage, causing high expression of MT-1 in BEAS-2B cells. Moreover, the expression of BAX increased, and the expression of BCL-2 decreased. Collectively, our data suggests the apoptosis effect of synergistic effects of CB and Cd exposure.

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“…Moreover, the proliferation index and ALP activity in highdose M. officinalis polysaccharide group were higher than those in low-dose M. officinalis polysaccharide group (p<0.05), implying that high-dose (75 mg/kg) M. officinalis polysaccharides are better in increasing ALP activity and have a more significant inducing effect on the proliferation of osteoblasts. A study demonstrated that the increase in bone metabolism markers suggests the accelerated bone transformation and bone loss [14] . Serum calcium and phosphorus are the important bone mineral compositions and their stable content is the essential sign of balance between bone resorption and bone formation [15] .…”

Section: Resultsmentioning

confidence: 99%

Effects of Morinda officinalis Polysaccharides on the Proliferation and Viability of Osteoblasts in Rats with Osteoporosis

Qiao¹,

Li²,

Tong³

2022

IJPS

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Qiao et al.: Effects of Morinda officinalis Polysaccharides in RatsWe aimed to evaluate the effects of Morinda officinalis polysaccharides on the proliferation and viability of osteoblasts in rats with osteoporosis. 24 non-parous Sprague-Dawley rats were assigned into normal, model, low-dose and high-dose Morinda officinalis polysaccharide groups. The model of osteoporosis was established by ovariectomy. After successful modeling, 50 mg/kg and 75 mg/kg Morinda officinalis polysaccharides were subcutaneously injected in low-dose and high-dose Morinda officinalis polysaccharide groups, respectively. After 3 w of intervention, osteoblasts were prepared using the skull and their proliferation index and alkaline phosphatase activity were measured. The histopathological changes in femoral tissues were observed. The bone mineral density and levels of serum osteocalcin, calcium and phosphorus, as well as the protein levels of receptor activator of nuclear factor kappa-B, receptor activator of nuclear factor kappa-B ligand and osteoprotegerin were determined. The proliferation index and alkaline phosphatase activity were highest in high-dose Morinda officinalis polysaccharide group (p<0.05). Bone tissues had decreased cell spaces and gradually reduced spaces between the medullary cavities inMorinda officinalis polysaccharide groups. Model group had the highest bone mineral density and levels of serum osteocalcin, calcium and phosphorus (p<0.05). The protein expression levels of receptor activator of nuclear factor kappa-B ligand and receptor activator of nuclear factor kappa-B showed a decreasing trend, while that of osteoprotegerin displayed a rising trend sequentially in model, low-dose, high-dose Morinda officinalis polysaccharide and normal groups (p<0.05). Morinda officinalis polysaccharides promotes the proliferation of osteoblasts, enhances their viability and maintains the balance between bone resorption and formation in rats with osteoporosis probably by modulating the protein expression of the receptor activator of nuclear factor kappa-B ligand/receptor activator of nuclear factor kappa-B/osteoprotegerin pathway.

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Carbon black-induced detrimental effect on osteoblasts at low concentrations: Remarkably compromised differentiation without significant cytotoxicity

Cited by 15 publications

References 46 publications

Binding of Benzo[a]pyrene Alters the Bioreactivity of Fine Biochar Particles toward Macrophages Leading to Deregulated Macrophagic Defense and Autophagy

Binding of Benzo[a]pyrene Alters the Bioreactivity of Fine Biochar Particles toward Macrophages Leading to Deregulated Macrophagic Defense and Autophagy

Low-dose Exposure to Black Carbon and Cadmium Significantly Induce Lung Injury by Promoting Cellular Apoptosis

Effects of Morinda officinalis Polysaccharides on the Proliferation and Viability of Osteoblasts in Rats with Osteoporosis

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