Carbon dioxide fixation by Chlorella sp. USTB-01 with a fermentor-helical combined photobioreactor

Abstract

A promising microalgal strain isolated from fresh water, which can grow both autotrophically on inorganic carbon under lighting and heterotrophically on organic carbon without lighting, was identified as Chlorella sp. USTB-01 with the phylogenetic analysis based on 18S ribosomal ribonucleic acid (rRNA) gene sequences. In the heterotrophic batch culture, more than 20.0 g$L -1 of cell dry weight concentration (DWC) of Chlorella sp. USTB-01 was obtained at day 5, and which was used directly to seed the autotrophic culture. A novel fermentor-helical combined photobioreactor was established and used to cultivate Chlorella sp. USTB-01 for the fixation of carbon dioxide (CO 2 ). It showed that the autotrophic growth of Chlorella sp. USTB-01 in the combined photobioreactor was more effective than that in the fermentor alone and the maximum DWC of 2.5 g$L -1 was obtained at day 6. The highest CO 2 fixation of 95% appeared on day 1 in the exponential growth phases of Chlorella sp. USTB-01 and 49.8% protein was found in the harvested microalgal cells.

Microbial Electrochemical CO2 Reduction and In-Situ Biogas Upgrading at Various pH Conditions

“…So, it is critical to seek for economically green and efficient CO 2 conversion technologies, which will also be the development trend in energy gas treatment in Europe in subsequent decades [2]. The main technologies for CO 2 conversion include biotransformation [5], catalytic hydrogenation [6], photochemical or photoelectrochemical reduction [7], and electrochemical reduction [8,9]. Microbial electrochemistry is an emerging electrochemical reduction technology.…”

Influence of Surface Zeta Potential on Adhesion of <i>Chlorella sp. to Substratum Surfaces</i>

“…As shown in figure 1, a very promising microalgal strain, the freshwater autotrophic Chlorella sp. which is spheroidal in shape, were obtained from Department of Biological Science and Technology at the University of Science and Technology Beijing [12]. The substratum surfaces used in the study were the ordinary microscope glass slide and the postive charge coated microscope glass slide.…”

“…were cultivated autotrophically in the modification Bold's Basal medium [13] in a 5 l photobioreactor at 25±1 ℃ under continuous irradiance of 120 µmol.m -2 .s and continuous aeration rate of 15 ml/min by a pump. Once the algal cell number concentration reached 1×10 12 cells/m 3 during their exponential growth phase, certain amount of Chlorella sp. suspensions were put into a sterilized beaker.…”

Increasing the autotrophic growth of Chlorella USTB ‐01 via the control of bacterial contamination by Bdellovibrio USTB ‐06

“…Furthermore microalgae can also be used for bioremediation of gas pollution such as CO 2 (Demirbas ; Jia et al . ), indicating that microalgae can be cultured autotrophically in open ponds or photobioreactors by utilizing solar energy and fixing CO 2 . Moreover, the phototrophic cultivation of microalgae has been commercialized for high value‐added products that are used as human and animal nutrition supplement (Skjånes et al .…”

“…As a green and alternative renewable diesel fuel, microalgal biofuel is therefore prized and believed to bring benefits to human beings and the environment (Patil et al 2008;Ahmad et al 2011). Furthermore microalgae can also be used for bioremediation of gas pollution such as CO 2 (Demirbas 2011;Jia et al 2011), indicating that microalgae can be cultured autotrophically in open ponds or photobioreactors by utilizing solar energy and fixing CO 2 . Moreover, the phototrophic cultivation of microalgae has been commercialized for high value-added products that are used as human and animal nutrition supplement (Skj anes et al 2013).…”