Carbon dioxide sequestration by chemolithotrophic oleaginous bacteria for production and optimization of polyhydroxyalkanoate

Kumar, Manish; Gupta, Asmita; Thakur, Indu Shekhar

doi:10.1016/j.biortech.2016.02.038

Cited by 73 publications

(24 citation statements)

References 27 publications

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“…Helium was used as carrier gas at a flow rate of 2 mL/min. Samples of 5 µL were injected at an inlet temperature of 60 • C. The oven temperature was kept at 60 • C for 3 min and then increased to 280 • C at a rate of 12 • C/min and held at 280 • C for 8 min [31]. All samples were diluted 10 times with chloroform and filtered over a 0.2 µm PTFE membrane prior to analysis.…”

Section: Gas Chromatography-mass Spectroscopy (Gc-ms)mentioning

confidence: 99%

Thauera aminoaromatica MZ1T Identified as a Polyhydroxyalkanoate-Producing Bacterium within a Mixed Microbial Consortium

et al. 2020

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Polyhydroxyalkanoates (PHAs) form a highly promising class of bioplastics for the transition from fossil fuel-based plastics to bio-renewable and biodegradable plastics. Mixed microbial consortia (MMC) are known to be able to produce PHAs from organic waste streams. Knowledge of key-microbes and their characteristics in PHA-producing consortia is necessary for further process optimization and direction towards synthesis of specific types of PHAs. In this study, a PHA-producing mixed microbial consortium (MMC) from an industrial pilot plant was characterized and further enriched on acetate in a laboratory-scale selector with a working volume of 5 L. 16S-rDNA microbiological population analysis of both the industrial pilot plant and the 5 L selector revealed that the most dominant species within the population is Thauera aminoaromatica MZ1T, a Gram-negative beta-proteobacterium belonging to the order of the Rhodocyclales. The relative abundance of this Thauera species increased from 24 to 40% after two months of enrichment in the selector-system, indicating a competitive advantage, possibly due to the storage of a reserve material such as PHA. First experiments with T. aminoaromatica MZ1T showed multiple intracellular granules when grown in pure culture on a growth medium with a C:N ratio of 10:1 and acetate as a carbon source. Nuclear magnetic resonance (NMR) analyses upon extraction of PHA from the pure culture confirmed polyhydroxybutyrate production by T. aminoaromatica MZ1T.

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Section: Gas Chromatography-mass Spectroscopy (Gc-ms)mentioning

confidence: 99%

Thauera aminoaromatica MZ1T Identified as a Polyhydroxyalkanoate-Producing Bacterium within a Mixed Microbial Consortium

et al. 2020

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“…Oleaginous bacteria has the advantage of the highest growth rate over other oleaginous microorganisms, and can reach high cell density within 24 h when grown in simple culture medium. However, unlike TAG as lipid storage materials in eukaryotes, most of the reported oleaginous bacteria accumulate neutral lipids and specifically store poly hydroxyl alkanoates (PHA) as their lipid storage compounds . A review by Wáltermann and Steinbüchel detailed neutral lipid bodies accumulation in prokaryotes and discussed the relationship of bacterial neutral lipid bodies with eukaryotic lipids.…”

Section: Oleaginous Microorganismsmentioning

confidence: 99%

“…However, unlike TAG as lipid storage materials in eukaryotes, most of the reported oleaginous bacteria accumulate neutral lipids and specifically store poly hydroxyl alkanoates (PHA) as their lipid storage compounds. 70 A review by Wáltermann and Steinbüchel 71 detailed neutral lipid bodies accumulation in prokaryotes and discussed the relationship of bacterial neutral lipid bodies with eukaryotic lipids. Another review by Alverez and Steinbüchel 72 emphasized the occurrence and biotechnological potential of triacylglycerols (TAG) in prokaryotes, especially in actinomycetes.…”

Section: Oleaginous Bacteriamentioning

confidence: 99%

Role of flow cytometry for the improvement of bioprocessing of oleaginous microorganisms

Sonowal

Chikkaputtaiah

Velmurugan

2019

J of Chemical Tech & Biotech

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Oleaginous microorganisms, such as yeast, fungi, microalgae and bacteria, represent a key segment of second generation feed‐stock materials and are considered to synthesize a wide range of industrially important chemical compounds. Oleaginous microorganisms possess a broad varieties of chemical compounds such as carotenoids, pigments, carbohydrates, chlorophyll, and storage‐material lipids. Oleaginous microorganisms have been recognized as promising sources for the synthesis of unsaturated, especially polyunsaturated fatty acids (PUFA). So far, a variety of high‐throughput screening methodologies (HTMs) have been employed for the development of bioprocessing of oleaginous microorganisms for sustainable production of industrially valuable compounds. Of HTMs, flow cytometry (FC) and sorters (FACS) have received substantial interest as better HTMs because of their ability to screen large numbers of cells within seconds, and interrogate and isolate living cells at single‐cell level. Forward and side scattering signals of FC are used to determine the physiological state of the cell while different channels available in the FC facilitate the detection of signals produced from fluorophores. Simultaneous measurement of physiological characteristics along with specific compound accumulation at single‐cell level enables the possibility of separating a particular phenotype with specific properties from a population. Different microbial strain development strategies in combination with FACS produced improved phenotypes with desired properties. This review first summarizes the FACS methodologies suitable for oleaginous microorganisms and the significant progress that has been achieved in oleaginous microorganisms using FACS, and highlights the important, advanced and future prospects of FACS methodologies that are suitable for the development of bioprocessing in oleaginous microorganisms. © 2018 Society of Chemical Industry

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“…The bacterium is characterized by chemolithotrophic fixation of carbon dioxide which is supported by the presence of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo), carbonic anhydrase, and carboxylases ( 4 ). The strain was studied in detail for CO 2 sequestration along with biodiesel and polyhydroxyalkanoate (PHA) production ( 5 , 6 ).…”

Section: Genome Announcementmentioning

confidence: 99%

“…Enzymes for fatty acid metabolism such as acetyl-CoA carboxlases, malonyl Co-ACP transacylase, 3-ketoacyl ACP-synthase, and 3-ketoacyl ACP-reductase are present ( 4 ). Enzymes involved in PHA synthesis like β-ketoacyl-CoA thiolase and acetoacetyl-CoA dehydrogenase were also identified in the genome sequence ( 6 ). Therefore, this strain can be applied to sequester CO 2 and value-added products.…”

Section: Genome Announcementmentioning

confidence: 99%

Genome Sequence of Carbon Dioxide-Sequestering Serratia sp. Strain ISTD04 Isolated from Marble Mining Rocks

et al. 2016

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Carbon dioxide sequestration by chemolithotrophic oleaginous bacteria for production and optimization of polyhydroxyalkanoate

Cited by 73 publications

References 27 publications

Thauera aminoaromatica MZ1T Identified as a Polyhydroxyalkanoate-Producing Bacterium within a Mixed Microbial Consortium

Thauera aminoaromatica MZ1T Identified as a Polyhydroxyalkanoate-Producing Bacterium within a Mixed Microbial Consortium

Role of flow cytometry for the improvement of bioprocessing of oleaginous microorganisms

Genome Sequence of Carbon Dioxide-Sequestering Serratia sp. Strain ISTD04 Isolated from Marble Mining Rocks

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