Role of flow cytometry for the improvement of bioprocessing of oleaginous microorganisms

Abstract

Oleaginous microorganisms, such as yeast, fungi, microalgae and bacteria, represent a key segment of second generation feed‐stock materials and are considered to synthesize a wide range of industrially important chemical compounds. Oleaginous microorganisms possess a broad varieties of chemical compounds such as carotenoids, pigments, carbohydrates, chlorophyll, and storage‐material lipids. Oleaginous microorganisms have been recognized as promising sources for the synthesis of unsaturated, especially polyunsaturated fatty acids (PUFA). So far, a variety of high‐throughput screening methodologies (HTMs) have been employed for the development of bioprocessing of oleaginous microorganisms for sustainable production of industrially valuable compounds. Of HTMs, flow cytometry (FC) and sorters (FACS) have received substantial interest as better HTMs because of their ability to screen large numbers of cells within seconds, and interrogate and isolate living cells at single‐cell level. Forward and side scattering signals of FC are used to determine the physiological state of the cell while different channels available in the FC facilitate the detection of signals produced from fluorophores. Simultaneous measurement of physiological characteristics along with specific compound accumulation at single‐cell level enables the possibility of separating a particular phenotype with specific properties from a population. Different microbial strain development strategies in combination with FACS produced improved phenotypes with desired properties. This review first summarizes the FACS methodologies suitable for oleaginous microorganisms and the significant progress that has been achieved in oleaginous microorganisms using FACS, and highlights the important, advanced and future prospects of FACS methodologies that are suitable for the development of bioprocessing in oleaginous microorganisms. © 2018 Society of Chemical Industry

Comparison of Cellular Autofluorescence Patterns of Two Model Microalgae by Flow Cytometry

“…As similar, a typical automated cell counter was further optimized for the facilitated quantification of chlorophyll content in microalgae [24]. Nevertheless, our previous work illustrated that although automated cell counter was not favorably effective, flow cytometry-based analysis was revealed as the best analytical instrument to work with microalgae [25], as also commonly stressed by other researchers [26][27][28].…”

Aplikasi Flow Cytometry Dalam Bidang Imunologi: Review

“…Namun, penggunaan senyawa tersebut tidak selalu diinginkan, karena membuat kondisi pengujian yang kurang fisiologis (Thomas, et al, 2019). Tantangan lainnya yaitu flow cytometry terbatas hanya untuk sampel cair, sehingga sebelum melakukan analisis, sel harus dibuat secara menyeluruh dalam cairan (Sonowal, et al, 2018). Kecepatan deteksi dari flow cytometry juga berubah menjadi lebih lambat ketika perkembangan ukuran instrumen menjadi lebih kecil (Gong, et al, 2018).…”

Symbiotic Associations: Key Factors That Determine Physiology and Lipid Accumulation in Oleaginous Microorganisms

“…In general, two phases are involved in accumulation of excessive amount of intracellular fatty acids in oleaginous microorganisms (Davies and Holdsworth, 1992). The oleaginous microbe consumes available nitrogen for cell growth and proliferation in the first stage and further converts available carbon into intracellular fatty acids mainly under nitrogen limitation condition (Davies and Holdsworth, 1992;Sonowal et al, 2019). Symbiotic association helps to overcome few barriers associated with oleaginous microbes hence reducing cost associated with production of microbial biofuels.…”