Carbon nanodots based biosensors for gene mutation detection

García‐Mendiola, Tania; Bravo, Iria; López-Moreno, José María; Pariente, F.; Wannemacher, Reinhold; Weber, Karina; Popp, Jürgen; Lorenzo, Encarnación

doi:10.1016/j.snb.2017.10.105

Cited by 78 publications

(41 citation statements)

References 61 publications

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“…biosensors, nanomaterials are deposited on biosensors' electrode surfaces using various methods [65][66][67][68][69]. Figure 1 presents a schematic of the methods used for the deposition of nanomaterials onto electrode surfaces.…”

Section: Nanomaterials Deposition Methods Onto Electrode Surfacesmentioning

confidence: 99%

Deposition of nanomaterials: A crucial step in biosensor fabrication

Ahmad

Wolfbeis

Hahn

et al. 2018

Materials Today Communications

178

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 An easy, stable, and reproducible nanomaterial deposition/growth method is crucial for the realization of fabricated biosensor devices' performance, such as device stability, and reproducibility, as well as other sensing properties.  Key challenges for optimum deposition include stability, reproducibility, repeatability, and the ability of deposited nanomaterials to address these challenges is critiqued.

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Section: Nanomaterials Deposition Methods Onto Electrode Surfacesmentioning

confidence: 99%

Deposition of nanomaterials: A crucial step in biosensor fabrication

Ahmad

Wolfbeis

Hahn

et al. 2018

Materials Today Communications

178

View full text Add to dashboard Cite

show abstract

“…Among the fabricated biosensors based on carbon nanomaterials, a DNA biosensor using CNDs synthesized via a thermal carbonation method in a screen-printed disposable electrode was reported [62]. CNDs were composed of a hexagonal lattice of sp 2 carbons incorporated with sp 3 carbons at a size below 10 nm.…”

Section: Carbon-based Nanomaterials In Biosensorsmentioning

confidence: 99%

“…Biosensors based on carbon nanomaterials. (a) Schematic images of a DNA biosensor composed of carbon nanodots (CNDs) on a screen-printed disposable electrode to detect gene mutations, and differential pulse voltammetry (DPV) analysis of electrochemical detection performance (reproduced with permission from Reference[62], published by Elsevier, 2018). (b) An ultrasensitive electrochemical biosensor based on surface-modified graphene quantum dots (GQDs) to detect metal ions and small molecules, and DPV analysis of Hg(II), Cu(II), and Cd(II) detection (reproduced with permission from Reference[63], published by American Chemical Society, 2018).…”

mentioning

confidence: 99%

Highly Sensitive Biosensors Based on Biomolecules and Functional Nanomaterials Depending on the Types of Nanomaterials: A Perspective Review

et al. 2020

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Biosensors are very important for detecting target molecules with high accuracy, selectivity, and signal-to-noise ratio. Biosensors developed using biomolecules such as enzymes or nucleic acids which were used as the probes for detecting the target molecules were studied widely due to their advantages. For example, enzymes can react with certain molecules rapidly and selectively, and nucleic acids can bind to their complementary sequences delicately in nanoscale. In addition, biomolecules can be immobilized and conjugated with other materials by surface modification through the recombination or introduction of chemical linkers. However, these biosensors have some essential limitations because of instability and low signal strength derived from the detector biomolecules. Functional nanomaterials offer a solution to overcome these limitations of biomolecules by hybridization with or replacing the biomolecules. Functional nanomaterials can give advantages for developing biosensors including the increment of electrochemical signals, retention of activity of biomolecules for a long-term period, and extension of investigating tools by using its unique plasmonic and optical properties. Up to now, various nanomaterials were synthesized and reported, from widely used gold nanoparticles to novel nanomaterials that are either carbon-based or transition-metal dichalcogenide (TMD)-based. These nanomaterials were utilized either by themselves or by hybridization with other nanomaterials to develop highly sensitive biosensors. In this review, highly sensitive biosensors developed from excellent novel nanomaterials are discussed through a selective overview of recently reported researches. We also suggest creative breakthroughs for the development of next-generation biosensors using the novel nanomaterials for detecting harmful target molecules with high sensitivity.

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“…It was then kept at 4 • C until use. From the elemental analysis, a concentration of 3.5 µM for the stock solution was estimated [25].…”

Section: Synthesis Of Carbon Nanodotsmentioning

confidence: 99%

Enhanced Performance of Reagent-Less Carbon Nanodots Based Enzyme Electrochemical Biosensors

Bravo

Gutiérrez-Sánchez

García‐Mendiola

et al. 2019

Sensors

Self Cite

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This work reports on the advantages of using carbon nanodots (CNDs) in the development of reagent-less oxidoreductase-based biosensors. Biosensor responses are based on the detection of H2O2, generated in the enzymatic reaction, at 0.4 V. A simple and fast method, consisting of direct adsorption of the bioconjugate, formed by mixing lactate oxidase, glucose oxidase, or uricase with CNDs, is employed to develop the nanostructured biosensors. Peripherical amide groups enriched CNDs are prepared from ethyleneglycol bis-(2-aminoethyl ether)-N,N,N′,N′-tetraacetic acid and tris(hydroxymethyl)aminomethane, and used as precursors. The bioconjugate formed between lactate oxidase and CNDs was chosen as a case study to determine the analytical parameters of the resulting L-lactate biosensor. A linear concentration range of 3.0 to 500 µM, a sensitivity of 4.98 × 10−3 µA·µM−1, and a detection limit of 0.9 µM were obtained for the L-lactate biosensing platform. The reproducibility of the biosensor was found to be 8.6%. The biosensor was applied to the L-lactate quantification in a commercial human serum sample. The standard addition method was employed. L-lactate concentration in the serum extract of 0.9 ± 0.3 mM (n = 3) was calculated. The result agrees well with the one obtained in 0.9 ± 0.2 mM, using a commercial spectrophotometric enzymatic kit.

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Carbon nanodots based biosensors for gene mutation detection

Cited by 78 publications

References 61 publications

Deposition of nanomaterials: A crucial step in biosensor fabrication

Deposition of nanomaterials: A crucial step in biosensor fabrication

Highly Sensitive Biosensors Based on Biomolecules and Functional Nanomaterials Depending on the Types of Nanomaterials: A Perspective Review

Enhanced Performance of Reagent-Less Carbon Nanodots Based Enzyme Electrochemical Biosensors

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