Carbon nanohorns accelerate bone regeneration in rat calvarial bone defect

Kasai, Takefumi; Matsumura, Shigenobu; Iizuka, Tadashi; Shiba, Kiyotaka; Kanamori, Takeshi; Yudasaka, Masako; Iijima, Sumio; Yokoyama, Atsuro

doi:10.1088/0957-4484/22/6/065102

Cited by 32 publications

(35 citation statements)

References 44 publications

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“…521−524 MWCNTs have been reported by many research teams, including ours, to possess bone induction potential. 58,62,63,67,213,215,216,218,525,526 If conjugation with MWCNTs further improves the mechanical characteristics of PEEK and also induces osteogenesis, then MWCNT/PEEK composite will become an ideal interbody fusion material (Figure 13). …”

Section: Discussion and Perspectivementioning

confidence: 99%

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Safe Clinical Use of Carbon Nanotubes as Innovative Biomaterials

et al. 2014

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Section: Discussion and Perspectivementioning

confidence: 99%

“…The extent of osteogenesis was greater under the membrane containing carbon nanohorns than under the membrane without the carbon nanohorns, showing that carbon nanohorns accelerated bone regeneration. 216 …”

Section: Present Status Of Research Into the Application Of Cnts As Bmentioning

confidence: 99%

Safe Clinical Use of Carbon Nanotubes as Innovative Biomaterials

et al. 2014

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“…Carbon-based nanosubstrates, such as carbon nanotubes, 13,14 carbon nanohorns, 15 carbon nanofibers, 16 and graphene, 17,18 have been investigated widely for stem cell therapies and tissue engineering platforms because of their unique physical, chemical, and mechanical properties. Graphene oxide (GO) in a two-dimensional honeycomb lattice of a carbon monolayer is obtained by oxidation and exfoliation of graphite.…”

Section: Introductionmentioning

confidence: 99%

Graphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket

Nishida¹,

Miyaji²,

Kato³

et al. 2016

IJN

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Graphene oxide (GO) consisting of a carbon monolayer has been widely investigated for tissue engineering platforms because of its unique properties. For this study, we fabricated a GO-applied scaffold and assessed the cellular and tissue behaviors in the scaffold. A preclinical test was conducted to ascertain whether the GO scaffold promoted bone induction in dog tooth extraction sockets. For this study, GO scaffolds were prepared by coating the surface of a collagen sponge scaffold with 0.1 and 1 μg/mL GO dispersion. Scaffolds were characterized using scanning electron microscopy (SEM), physical testing, cell seeding, and rat subcutaneous implant testing. Then a GO scaffold was implanted into a dog tooth extraction socket. Histological observations were made at 2 weeks postsurgery. SEM observations show that GO attached to the surface of collagen scaffold struts. The GO scaffold exhibited an interconnected structure resembling that of control subjects. GO application improved the physical strength, enzyme resistance, and adsorption of calcium and proteins. Cytocompatibility tests showed that GO application significantly increased osteoblastic MC3T3-E1 cell proliferation. In addition, an assessment of rat subcutaneous tissue response revealed that implantation of 1 μg/mL GO scaffold stimulated cellular ingrowth behavior, suggesting that the GO scaffold exhibited good biocompatibility. The tissue ingrowth area and DNA contents of 1 μg/mL GO scaffold were, respectively, approximately 2.5-fold and 1.4-fold greater than those of the control. Particularly, the infiltration of ED2-positive (M2) macrophages and blood vessels were prominent in the GO scaffold. Dog bone-formation tests showed that 1 μg/mL GO scaffold implantation enhanced bone formation. New bone formation following GO scaffold implantation was enhanced fivefold compared to that in control subjects. These results suggest that GO was biocompatible and had high bone-formation capability for the scaffold. The GO scaffold is expected to be beneficial for bone tissue engineering therapy

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“…Recently it has been reported that carbon nanohorns (CNH; another carbon allotrope closely related to the fullerenes), fixated on a porous polytetrafluoroethylene membrane, were used for covering the calvarial bone defect in rats. The results showed the attachment of macrophages to CNHs and acceleration of newly formed bone regeneration in the presence of these nanoparticles [26]. …”

Section: Introductionmentioning

confidence: 99%

Growth and Potential Damage of Human Bone-Derived Cells on Fresh and Aged Fullerene C60 Films

Kopová

Bačáková

Lavrentiev

et al. 2013

IJMS

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Fullerenes are nanoparticles composed of carbon atoms arranged in a spherical hollow cage-like structure. Numerous studies have evaluated the therapeutic potential of fullerene derivates against oxidative stress-associated conditions, including the prevention or treatment of arthritis. On the other hand, fullerenes are not only able to quench, but also to generate harmful reactive oxygen species. The reactivity of fullerenes may change in time due to the oxidation and polymerization of fullerenes in an air atmosphere. In this study, we therefore tested the dependence between the age of fullerene films (from one week to one year) and the proliferation, viability and metabolic activity of human osteosarcoma cells (lines MG-63 and U-2 OS). We also monitored potential membrane and DNA damage and morphological changes of the cells. After seven days of cultivation, we did not observe any cytotoxic morphological changes, such as enlarged cells or cytosolic vacuole formation. Furthermore, there was no increased level of DNA damage. The increasing age of the fullerene films did not cause enhancement of cytotoxicity. On the contrary, it resulted in an improvement in the properties of these materials, which are more suitable for cell cultivation. Therefore, fullerene films could be considered as a promising material with potential use as a bioactive coating of cell carriers for bone tissue engineering.

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Carbon nanohorns accelerate bone regeneration in rat calvarial bone defect

Cited by 32 publications

References 44 publications

Safe Clinical Use of Carbon Nanotubes as Innovative Biomaterials

Safe Clinical Use of Carbon Nanotubes as Innovative Biomaterials

Graphene oxide scaffold accelerates cellular proliferative response and alveolar bone healing of tooth extraction socket

Growth and Potential Damage of Human Bone-Derived Cells on Fresh and Aged Fullerene C60 Films

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