Carbon nanotube/polysulfone composite screen-printed electrochemical enzyme biosensors

Abstract

The fabrication, evaluation and attractive performance of multiwall carbon nanotube(MWCNT)/polysulfone biocomposite membrane modified thick-film screen-printed electrochemical biosensors are reported. The fabricated carbon nanotube/polysulfone (CNT/PS) strips combine the attractive advantages of carbon nanotube materials, polysulfone matrix and disposable screen-printed electrodes. Such thick-film carbon nanotubes/polysulfone sensors have a well defined performance, are mechanically stable, and exhibit high electrochemical activity. Furthermore, biocompatibility of CNT/PS composite allows easy incorporation of biological functional moiety of horseradish peroxidase by phase inversion technique. The comparison of graphite with MWCNT as conductor material is described in this paper. The proposed H(2)O(2) biosensor exhibited a linear range (applied potential, -0.2 V) from 0.02 to 0.5 mM and a K(M)(app) of 0.71 mM.

An Amperometric d-Amino Acid Biosensor Prepared with a Thermostable D-Proline Dehydrogenase and a Carbon Nanotube-Ionic Liquid Gel

“…In other words, the reactivity of the enzyme-substrate in the CNT/d-Pro dH immobilized electrode was higher than those of the other carbon material/d-Pro dH immobilized electrodes. The smaller Km value for the CNT immobilized electrode is consistent with previous reports for CNT-modified enzyme sensors, such as CNT and lactate dehydrogenase (enzyme cofactor NAd), 27 HRP (enzyme cofactor heme), 17 and GOx (enzyme cofactor FAd) electrodes. 28,29 …”

“…For example, a CNT/glucose oxidase (GOx) doped polypyrrole immobilized electrode and a screen-printed electrode immobilized with CNT/horseradish peroxidase (HRP)/polysulfone were reported for glucose and H2O2 sensors, respectively. 16,17 Moreover, Fukushima et al recently developed a CNT gel, which was a mixture of a CNT and imidazolium ion based ionic liquids. 18 The CNT gel has a large potential for electrode materials because of its large surface area and easy membrane-forming performance.…”

Use of Redox Probes for Characterization of Layer-by-Layer Gold Nanoparticle-Modified Screen-Printed Carbon Electrodes

“…When employed as platforms for sensing and biosensing, such electrodes reportedly exhibit improved electrochemical properties (e.g., enhanced response and/or superior electron-transfer kinetics over bare SPCEs). 7,8,[16][17][18] One benefit provided by nanoparticles that is typically asserted as important in rationalizing the augmented response of nanomaterial-modified SPCEs over bare SPCEs is the increase in electroactive surface area provided by the inclusion of nanomaterials on the electrode surface. 7,8,[16][17][18] The electroactive surface area, A, is often determined (in cm 2 ) through voltammetric studies of common redox probes by employing the Randles-Ševčík equation (at 25…”

Facile Preparation of Carbon Nanotube/Poly(ethyl 2‐cyanoacrylate) Composite Electrode by Water‐Vapor‐Initiated Polymerization for Enhanced Amperometric Detection

“…Recently, Fàbregas et al developed a series of electrochemical biosensors based on CNT/polysulfone (CNT/PSf) composite for measuring some important biomarkers. [23][24][25][26][27][28] The sensing materials, fabrication approaches, determined analytes and electrochemical characterization of these CNT-based electrodes are summarized in Table 1.…”