Carbon nanotube-reinforced cell-derived matrix-silk fibroin hierarchical scaffolds for bone tissue engineering applications

Lemos, Rafael; Maia, F. Raquel; Ribeiro, Viviana P.; Costa, João B.; Coutinho, Paulo J. G.; Reis, Rui L.; Oliveira, Joaquím M.

doi:10.1039/d1tb01972d

Cited by 16 publications

(6 citation statements)

References 51 publications

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“…CNTs have been reported to possess excellent electrical conductivity and high mechanical properties; furthermore, the rod-like shape and nanoscale dimension of CNTs enable them to act as biomimetic analogues for fibrin of extracellular matrix. , Therefore, CNTs were introduced to the surface of the PLA inverse opal membrane to enhance the properties of the periosteum material. To coat the membrane with CNTs, the CNTs solution were evenly dropped onto the surface of the membrane and dried.…”

Section: Resultsmentioning

confidence: 99%

Artificial Periosteum with Oriented Surface Nanotopography and High Tissue Adherent Property

Sun,

Shang,

Guo

et al. 2023

ACS Appl. Mater. Interfaces

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Massive periosteal defects often significantly impair bone regeneration and repair, which have become a major clinical challenge. Unfortunately, current engineered periosteal materials can hardly currently focus on achieving high tissue adhesion property, being suitable for cell growth, and inducing cell orientation concurrently to meet the properties of nature periosteum. Additionally, the preparation of oriented surface nanotopography often relies on professional equipment. In this study, inspired by the oriented collagen structure of nature periosteum, we present a composite artificial periosteum with a layer of oriented nanotopography surface containing carbon nanotubes (CNTs), cross-linked with adhesive polydopamine (PDA) hydrogel on both terminals. An oriented surface structure that can simulate the oriented alignment of periosteal collagen fibers can be quickly and conveniently obtained via a simple stretching of the membrane in a water bath. With the help of CNTs, our artificial periosteum exhibits sufficient mechanical strength and desired oriented nanotopological structure surface, which further induces the directional arrangement of human bone marrow mesenchymal stem cells (hBMSCs) on the membrane. These oriented hBMSCs express significantly higher levels of osteogenic genes and proteins, while the resultant composite periosteum can be stably immobilized in vivo in the rat model of massive calvarial defect through the PDA hydrogel, which finally shows promising bone regeneration ability. We anticipate that the developed functional artificial periosteum has great potential in biomedical applications for the treatment of composite defects of the bone and periosteum.

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Section: Resultsmentioning

confidence: 99%

Artificial Periosteum with Oriented Surface Nanotopography and High Tissue Adherent Property

Sun,

Shang,

Guo

et al. 2023

ACS Appl. Mater. Interfaces

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“…Also a consequence of their electronic bandgap structure, certain SWCNT chiralities exhibit photoluminescence by absorbing light in the NIR-I and emitting in the NIR-II region. This makes them excellent reagents for biological imaging , and scaffolds for biosensing applications. , SWCNTs have also been employed as photoinduced drug delivery vessels, , gene and protein delivery vehicles, , nanopores, , adjuvant vaccines, and are used in tissue engineering applications. − …”

Section: Carbon Nanomaterials Synthesis and Characterizationmentioning

confidence: 99%

Carbon Nanomaterial Fluorescent Probes and Their Biological Applications

Krasley,

Li,

Galeana

et al. 2024

Chem. Rev.

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Fluorescent carbon nanomaterials have broadly useful chemical and photophysical attributes that are conducive to applications in biology. In this review, we focus on materials whose photophysics allow for the use of these materials in biomedical and environmental applications, with emphasis on imaging, biosensing, and cargo delivery. The review focuses primarily on graphitic carbon nanomaterials including graphene and its derivatives, carbon nanotubes, as well as carbon dots and carbon nanohoops. Recent advances in and future prospects of these fields are discussed at depth, and where appropriate, references to reviews pertaining to older literature are provided.

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“…The pores in the freeze-dried sponges are smaller than 100 μm, and the pores’ diameters may be altered by varying the freezing temperature, the solution’s pH, and the number of organic solvents used [ 79 ]. Pore diameters can be increased from 60 to 250 μm by several cycles of freezing and thawing [ 80 ]. Using gas-foaming or solvent-casting leaching techniques can improve control over the pore structure.…”

Section: Structural Diversification Of Silk Biomaterialsmentioning

confidence: 99%

Recent Developments of Silk-Based Scaffolds for Tissue Engineering and Regenerative Medicine Applications: A Special Focus on the Advancement of 3D Printing

Shabbirahmed

Sekar²,

Gomez

et al. 2023

Biomimetics

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Regenerative medicine has received potential attention around the globe, with improving cell performances, one of the necessary ideas for the advancements of regenerative medicine. It is crucial to enhance cell performances in the physiological system for drug release studies because the variation in cell environments between in vitro and in vivo develops a loop in drug estimation. On the other hand, tissue engineering is a potential path to integrate cells with scaffold biomaterials and produce growth factors to regenerate organs. Scaffold biomaterials are a prototype for tissue production and perform vital functions in tissue engineering. Silk fibroin is a natural fibrous polymer with significant usage in regenerative medicine because of the growing interest in leftovers for silk biomaterials in tissue engineering. Among various natural biopolymer-based biomaterials, silk fibroin-based biomaterials have attracted significant attention due to their outstanding mechanical properties, biocompatibility, hemocompatibility, and biodegradability for regenerative medicine and scaffold applications. This review article focused on highlighting the recent advancements of 3D printing in silk fibroin scaffold technologies for regenerative medicine and tissue engineering.

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Carbon nanotube-reinforced cell-derived matrix-silk fibroin hierarchical scaffolds for bone tissue engineering applications

Abstract: In bone tissue engineering, the development of advanced biomimetic scaffolds has led to the quest for biomotifs in scaffold design that better recreate bone matrix structure and composition and hierarchy...

Cited by 16 publications

References 51 publications

Artificial Periosteum with Oriented Surface Nanotopography and High Tissue Adherent Property

Artificial Periosteum with Oriented Surface Nanotopography and High Tissue Adherent Property

Carbon Nanomaterial Fluorescent Probes and Their Biological Applications

Recent Developments of Silk-Based Scaffolds for Tissue Engineering and Regenerative Medicine Applications: A Special Focus on the Advancement of 3D Printing

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