The aim of this study was to evaluate the effects of sodium hyaluronate (HY),
single-walled carbon nanotubes (SWCNTs) and HY-functionalized SWCNTs (HY-SWCNTs) on
the behavior of primary osteoblasts, as well as to investigate the deposition of
inorganic crystals on titanium surfaces coated with these biocomposites. Primary
osteoblasts were obtained from the calvarial bones of male newborn Wistar rats (5
rats for each cell extraction). We assessed cell viability using the
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay and by
double-staining with propidium iodide and Hoechst. We also assessed the formation of
mineralized bone nodules by von Kossa staining, the mRNA expression of bone repair
proteins, and the deposition of inorganic crystals on titanium surfaces coated with
HY, SWCNTs, or HY-SWCNTs. The results showed that treatment with these biocomposites
did not alter the viability of primary osteoblasts. Furthermore, deposition of
mineralized bone nodules was significantly increased by cells treated with HY and
HY-SWCNTs. This can be partly explained by an increase in the mRNA expression of type
I and III collagen, osteocalcin, and bone morphogenetic proteins 2 and 4.
Additionally, the titanium surface treated with HY-SWCNTs showed a significant
increase in the deposition of inorganic crystals. Thus, our data indicate that HY,
SWCNTs, and HY-SWCNTs are potentially useful for the development of new strategies
for bone tissue engineering.