Carbon Nanotubes in Macrophages: Imaging and Chemical Analysis by X-ray Fluorescence Microscopy

Bussy, Cyrill; Cambedouzou, Julien; Lanone, Sophie; Leccia, E.; Heresanu, Vasile; Pinault, Mathieu; Mayne-l'Hermite, M.; Brun, Nathalie; Mory, C.; Cotte, Marine; Doucet, J.; Boczkowski, Jorge; Launois, Pascale

doi:10.1021/nl800914m

Cited by 60 publications

(42 citation statements)

References 39 publications

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“…metallic, ceramic). Furthermore, the ability of SXRF to map changes in elements integral to cellular homoeostasis [20], even at the sub-cellular level [5], could provide integrated understanding of nanoparticle-single-cell population interactions.…”

Section: Resultsmentioning

confidence: 99%

“…Microscopy and/or spectroscopy techniques and stereology have been applied to track and follow the interactions of nanoparticles within single cells [3,4]. Recently, X-ray fluorescence (XRF) microscopy at synchrotron radiation light sources has enabled the characterization of nanoparticle-cell interactions at the sub-cellular level [5,6]. Synchrotron X-ray fluorescence (SXRF) microscopy is a versatile analytical tool widely used in biomedical research and employs hard X-rays to excite and detect characteristic Ka X-ray fluorescence of important elements in medicine [5,6].…”

Section: Introductionmentioning

confidence: 99%

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Quantification of single-cell nanoparticle concentrations and the distribution of these concentrations in cell population

Rashkow

Patel

Tappero

et al. 2014

J. R. Soc. Interface.

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Quantification of nanoparticle uptake into cells is necessary for numerous applications in cellular imaging and therapy. Herein, synchrotron X-ray fluorescence (SXRF) microscopy, a promising tool to quantify elements in plant and animal cells, was employed to quantify and characterize the distribution of titanium dioxide (TiO 2 ) nanosphere uptake in a population of single cells. These results were compared with average nanoparticle concentrations per cell obtained by widely used inductively coupled plasma mass spectrometry (ICP-MS). The results show that nanoparticle concentrations per cell quantified by SXRF were of one to two orders of magnitude greater compared with ICP-MS. The SXRF results also indicate a Gaussian distribution of the nanoparticle concentration per cell. The results suggest that issues relevant to the field of single-cell analysis, the limitation of methods to determine physical parameters from large population averages leading to potentially misleading information and the lack of any information about the cellular heterogeneity are equally relevant for quantification of nanoparticles in cell populations.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantification of single-cell nanoparticle concentrations and the distribution of these concentrations in cell population

Rashkow

Patel

Tappero

et al. 2014

J. R. Soc. Interface.

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“…SR-XRF can be used not only to confirm the intracellular distribution of nanoparticles, but also as a unique tool to quantify intracellular nanoparticles (Thurn et al 2009). The toxicology of nanoparticles was investigated using micro-SR-XRF, showing an excess of calcium in macrophages exposed to carbon nanotubes (Bussy et al 2008). In a different field of application, uranium biogenic nanoparticles were evidenced in the outer membrane of metal-reducing bacteria (Marshall et al 2006).…”

Section: Examples Of Applicationmentioning

confidence: 99%

Bio-metals imaging and speciation in cells using proton and synchrotron radiation X-ray microspectroscopy

Ortega

Devès

Carmona

2009

J. R. Soc. Interface.

141

131

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The direct detection of biologically relevant metals in single cells and of their speciation is a challenging task that requires sophisticated analytical developments. The aim of this article is to present the recent achievements in the field of cellular chemical element imaging, and direct speciation analysis, using proton and synchrotron radiation X-ray micro-and nanoanalysis. The recent improvements in focusing optics for MeV-accelerated particles and keV X-rays allow application to chemical element analysis in subcellular compartments. The imaging and quantification of trace elements in single cells can be obtained using particle-induced X-ray emission (PIXE). The combination of PIXE with backscattering spectrometry and scanning transmission ion microscopy provides a high accuracy in elemental quantification of cellular organelles. On the other hand, synchrotron radiation X-ray fluorescence provides chemical element imaging with less than 100 nm spatial resolution. Moreover, synchrotron radiation offers the unique capability of spatially resolved chemical speciation using micro-X-ray absorption spectroscopy. The potential of these methods in biomedical investigations will be illustrated with examples of application in the fields of cellular toxicology, and pharmacology, bio-metals and metal-based nano-particles.

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“…The application of nanotechnology in medicine has potential in a range of treatments, including gene therapy, 1 drug delivery, 2 and medical devices. 3 However, turning the early promise into readily available medical treatments is a lengthy process, involving the development of the products efficacy, safety, stability, manufacture, and regulation. The tissueengineering nanomaterials, which are produced from traditional tissue-engineering materials by nanotechnology, have special biological properties and have attracted much interest.…”

Section: Introductionmentioning

confidence: 99%

Single-walled carbon nanotubes promote rat vascular adventitial fibroblasts to transform into myofibroblasts by SM22-α expression

Liu¹,

Liu²,

Xi³

et al. 2012

IJN

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Abstract:The aim of this study was to explore whether single-wall carbon nanotubes (SWCNTs) can be used as artery tissue-engineering materials by promoting vascular adventitial fibroblasts (VAFs) to transform into myofibroblasts (MFs) and to find the signal pathway involved in this process. VAFs were primary cultured and incubated with various doses of SWCNTs suspension (0, 0.8, 3.2, 12.5, 50, and 200 µg/mL). In the present study, we used three methods (MTT, WST-1, and WST-8) at the same time to detect the cell viability and immunofluorescence probe technology to investigate the effects of oxidative injury after VAFs incubated with SWCNTs. Immunocytochemical staining was used to detect SM 22 -α expression to confirm whether VAFs transformed into MFs. The protein levels were detected by western blotting. The results of immunocytochemical staining showed that SM 22 -α was expressed after incubation with 50 µg/mL SWCNTs for 96 hours, but with oxidative damage. The mRNA and protein levels of SM 22 -α, C-Jun N-terminal kinase, TGF-β 1 , and TGF-β receptor II in VAFs increased with the dose of SWCNTs. The expression of the p-Smad2/3 protein was upregulated while the Smad7 protein was significantly down-regulated. Smad4 was translocated to the nucleus to regulate SM 22 -α gene expression. In conclusion, SWCNTs promoted VAFs to transform into MFs with SM 22 -α expression by the C-Jun N-terminal kinase/Smads signal pathway at the early stage (48 hours) but weakened quickly. SWCNTs also promoted the transformation by the TGF-β l /Smads signal pathway at the advanced stage in a persistent manner. These results indicate that SWCNTs can possibly be used as artery tissue-engineering materials.

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Carbon Nanotubes in Macrophages: Imaging and Chemical Analysis by X-ray Fluorescence Microscopy

Cited by 60 publications

References 39 publications

Quantification of single-cell nanoparticle concentrations and the distribution of these concentrations in cell population

Quantification of single-cell nanoparticle concentrations and the distribution of these concentrations in cell population

Bio-metals imaging and speciation in cells using proton and synchrotron radiation X-ray microspectroscopy

Single-walled carbon nanotubes promote rat vascular adventitial fibroblasts to transform into myofibroblasts by SM22-α expression

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Carbon Nanotubes in Macrophages: Imaging and Chemical Analysis by X-ray Fluorescence Microscopy

Cited by 60 publications

References 39 publications

Quantification of single-cell nanoparticle concentrations and the distribution of these concentrations in cell population

Quantification of single-cell nanoparticle concentrations and the distribution of these concentrations in cell population

Bio-metals imaging and speciation in cells using proton and synchrotron radiation X-ray microspectroscopy

Single-walled carbon nanotubes promote rat vascular adventitial fibroblasts to transform into myofibroblasts by SM22-&alpha; expression

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Single-walled carbon nanotubes promote rat vascular adventitial fibroblasts to transform into myofibroblasts by SM22-α expression