Carbon quantum dots encapsulated in super small platinum nanocrystals core-shell architecture/nitrogen doped graphene hybrid nanocomposite for electrochemical biosensing of DNA damage biomarker-8-hydroxy-2′-deoxyguanosine

Zhao, Qiuyue; Fu, Morgan; Fan, Xiulin; Lu, Haijun; Wang, Haiyang; Zhang, Yufan; Wang, Huan

doi:10.1016/j.aca.2018.09.039

Cited by 23 publications

(8 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nitrogen-doped graphenes have been intensively investigated for their electrochemical behavior due to their capacity to improve electrical conductivity [36]. N-graphene exhibited enhanced electro-catalytic activity and showed potential applications in electrochemical sensors (8-hydroxy-2 -deoxyguanosine, adenine, guanine, thymine and cytosine) [38,39] and energy devices [40,41].…”

Section: Introductionmentioning

confidence: 99%

Nitrogen-Doped Graphene: The Influence of Doping Level on the Charge-Transfer Resistance and Apparent Heterogeneous Electron Transfer Rate

Coroș

Varodi

Pogăcean

et al. 2020

Sensors

View full text Add to dashboard Cite

Three nitrogen-doped graphene samples were synthesized by the hydrothermal method using urea as doping/reducing agent for graphene oxide (GO), previously dispersed in water. The mixture was poured into an autoclave and placed in the oven at 160 °C for 3, 8 and 12 h. The samples were correspondingly denoted NGr-1, NGr-2 and NGr-3. The effect of the reaction time on the morphology, structure and electrochemical properties of the resulting materials was thoroughly investigated using scanning electron microscopy (SEM) Raman spectroscopy, X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), elemental analysis, Cyclic Voltammetry (CV) and electrochemical impedance spectroscopy (EIS). For NGr-1 and NGr-2, the nitrogen concentration obtained from elemental analysis was around 6.36 wt%. In the case of NGr-3, a slightly higher concentration of 6.85 wt% was obtained. The electrochemical studies performed with NGr modified electrodes proved that the charge-transfer resistance (Rct) and the apparent heterogeneous electron transfer rate constant (Kapp) depend not only on the nitrogen doping level but also on the type of nitrogen atoms found at the surface (pyrrolic-N, pyridinic-N or graphitic-N). In our case, the NGr-1 sample which has the lowest doping level and the highest concentration of pyrrolic-N among all nitrogen-doped samples exhibits the best electrochemical parameters: a very small Rct (38.3 Ω), a large Kapp (13.9 × 10−2 cm/s) and the best electrochemical response towards 8-hydroxy-2′-deoxyguanosine detection (8-OHdG).

show abstract

Section: Introductionmentioning

confidence: 99%

Nitrogen-Doped Graphene: The Influence of Doping Level on the Charge-Transfer Resistance and Apparent Heterogeneous Electron Transfer Rate

Coroș

Varodi

Pogăcean

et al. 2020

Sensors

View full text Add to dashboard Cite

show abstract

“…A lower detection limit of 0.45 nM was reported in 2018 by Zhang et al [76]. who encapsulated CQDs in ultra-small platinum nanocrystals over nitrogen-doped graphene to form hybrid nanocomposites.…”

Section: Dna Sensingmentioning

confidence: 89%

“…who encapsulated CQDs in ultra-small platinum nanocrystals over nitrogen-doped graphene to form hybrid nanocomposites. The prepared nanocomposites were used for the detection of 8-OH-dG in human urine as well as damaged DNA and guanine, respectively, addressing the intensity change of the electrochemical signal of 8-OH-dG with DNA damage [76]. Despite the fact that the CDs have been used for DNA sensing, the work is in an early stage and complicated in terms of the preparation methods and number of materials used.…”

Section: Dna Sensingmentioning

confidence: 99%

Advances in fluorescent carbon dots for biomedical applications

Koutsogiannis

Thomou

Stamatis

et al. 2020

Advances in Physics: X

View full text Add to dashboard Cite

Carbon Dots are an emerging class of carbon-based nanoparticles, which since their discovery have attracted tremendous attention because of their exceptional fluorescent, chemical and mechanical properties as well as high photostability and biocompatibility. This unique combination of outstanding characteristics, together with the ease with which they can be synthesized, qualify carbon dots as highly promising materials for applications in electronics and biology, in particular, for biosensing, bioimaging, biotherapy and drug delivery. In this review, we present some of the most recent applications of carbon dots in biology and medicine, concentrating on their fluorescence properties, biocompatibility and efficiency; we also discuss how improvements could prompt their use in human studies. We illustrate how carbon dots, prepared through several facile and costeffective methods by either the bottom-up or the top-down route, can be used for imaging cells and bacteria and as sensing probes of metal cations. Moreover, we explain how their astonishing versatility has given rise to new biotherapy methods especially in the field of cancer theranostics.

show abstract

“…With a dissociation constant (KD) on the order of 0.1 μmol/L, the identified anti-8-oxo-dG aptamer had a high affinity for 8-oxo-dG . Due to the highly specific binding of the anti-8-oxo-dG aptamer to 8-oxo-dG, this aptamer has been applied in several approaches for the detection of 8-oxo-dG. ,− …”

Section: Introductionmentioning

confidence: 99%

Fluorescent Aptasensor for Determination of 8-Oxo-2′-deoxyguanosine in Urine Using Carbon Dots and Amine-Functionalized Gold Nanoparticles

Ngernpimai

Thonghlueng

Phothikul

et al. 2023

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

8-Oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dG) is a crucial biomarker of oxidative DNA damage. Herein, a highly sensitive fluorescent aptasensor for detecting 8-oxo-dG in urine was developed based on aptamer-mediated quenching of amino-terminated oligo(ethylene glycol)-capped gold nanoparticles (NH2-TEG-AuNPs) toward green emissive carbon dots (G-CDs). G-CDs were employed as fluorometric reporters. Upon addition of 8-oxo-dG, it formed a complex with an anti-8-oxo-dG aptamer, resulting in the quenching of fluorescence as NH2-TEG-AuNPs absorbed the emission from G-CDs. Initially, NH2-TEG-AuNPs suppressed the fluorescence intensity of G-CDs via the inner-filter effect (IFE). With the addition of the aptamers to a mixture of G-CDs and NH2-TEG-AuNPs, the electrostatic interactions between the aptamer and NH2-TEG-AuNPs resulted in the aggregation of NH2-TEG-AuNPs and the recovery of the fluorescence intensity of the quenched G-CDs. NH2-TEG-AuNPs dispersed due to the high affinity between the aptamer and 8-oxo-dG molecules, and the system demonstrated a fluorescence ″turn-off″ response of G-CDs. Thus, it was possible to determine the 8-oxo-dG concentration. A fluorescence calibration curve was constructed with two linear ranges: 100–2000 and 2000–10,000 nM. The limit of detection (LOD) for 8-oxo-dG was 15.89 nM. This approach was successfully applied to assess 8-oxo-dG in synthetic urine samples with mean recoveries ranging from 99 to 120% and relative standard deviations (RSDs) between 1.1 and 4.1%. The analytical performance was comparable to a commercially available enzyme-linked immunosorbent assay (ELISA) kit. This proposed fluorescent aptasensor for 8-oxo-dG detection in urine exhibited high selectivity and sensitivity over a broad concentration range against the target molecule and has the potential to be developed as a new platform for rapid screening of 8-oxo-dG.

show abstract

Carbon quantum dots encapsulated in super small platinum nanocrystals core-shell architecture/nitrogen doped graphene hybrid nanocomposite for electrochemical biosensing of DNA damage biomarker-8-hydroxy-2′-deoxyguanosine

Cited by 23 publications

References 48 publications

Nitrogen-Doped Graphene: The Influence of Doping Level on the Charge-Transfer Resistance and Apparent Heterogeneous Electron Transfer Rate

Nitrogen-Doped Graphene: The Influence of Doping Level on the Charge-Transfer Resistance and Apparent Heterogeneous Electron Transfer Rate

Advances in fluorescent carbon dots for biomedical applications

Fluorescent Aptasensor for Determination of 8-Oxo-2′-deoxyguanosine in Urine Using Carbon Dots and Amine-Functionalized Gold Nanoparticles

Contact Info

Product

Resources

About