Carbon source requirements for mating and mating‐type switching in the methylotrophic yeasts <i>Ogataea (Hansenula) polymorpha</i> and <i>Komagataella phaffii (Pichia pastoris)</i>

Feng, Dahao; Stoyanov, Anton; Olliff, Juliana C.; Wolfe, Kenneth H.; Lahtchev, Kantcho; Hanson, Sara J.

doi:10.1002/yea.3446

Cited by 6 publications

(2 citation statements)

References 38 publications

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“…A different type of mating type switching system, called the flip/flop inversion system, was discovered more recently in some species of fungi belonging to the genus Ogataea , such as O. polymorpha , O. minuta , and O. thermomethanolica ( 64 – 69 ) ( Fig. 1 B ).…”

Section: Organization and Evolution Of Mat Locimentioning

confidence: 99%

On the evolution of variation in sexual reproduction through the prism of eukaryotic microbes

Yadav

Sun

Heitman

2023

Proc. Natl. Acad. Sci. U.S.A.

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Almost all eukaryotes undergo sexual reproduction to generate diversity and select for fitness in their population pools. Interestingly, the systems by which sex is defined are highly diverse and can even differ between evolutionarily closely related species. While the most commonly known form of sex determination involves males and females in animals, eukaryotic microbes can have as many as thousands of different mating types for the same species. Furthermore, some species have found alternatives to sexual reproduction and prefer to grow clonally and yet undergo infrequent facultative sexual reproduction. These organisms are mainly invertebrates and microbes, but several examples are also present among vertebrates suggesting that alternative modes of sexual reproduction evolved multiple times throughout evolution. In this review, we summarize the sex-determination modes and variants of sexual reproduction found across the eukaryotic tree of life and suggest that eukaryotic microbes provide unique opportunities to study these processes in detail. We propose that understanding variations in modes of sexual reproduction can serve as a foundation to study the evolution of sex and why and how it evolved in the first place.

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Section: Organization and Evolution Of Mat Locimentioning

confidence: 99%

On the evolution of variation in sexual reproduction through the prism of eukaryotic microbes

Yadav

Sun

Heitman

2023

Proc. Natl. Acad. Sci. U.S.A.

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“…Sexual reproduction (mating and spore formation) is possible but can only be observed under nitrogen starvation conditions (Feng et al . 2020 ). In contrast to S. cerevisiae , where haploid cells mate spontaneously to form stable diploids, the mating-type ( MAT ) genes and most other mating-relevant genes of K. phaffii are not expressed in rich medium (Heistinger, Gasser and Mattanovich 2018 ).…”

Section: Mating and Mating-type Switchingmentioning

confidence: 99%

What makes Komagataella phaffii non-conventional?

Ata

Ergün

Fickers

et al. 2021

FEMS Yeast Research

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The important industrial protein production host Komagataella phaffii (syn Pichia pastoris) is classified as a non-conventional yeast. But what exactly makes K. phaffii non-conventional? In this review we set out to address the main differences to the ‘conventional’ yeast Saccharomyces cerevisiae, but also pinpoint differences to other non-conventional yeasts used in biotechnology. Apart from its methylotrophic lifestyle, K. phaffii is a Crabtree-negative yeast species. But even within the methylotrophs, K. phaffii possesses distinct regulatory features such as glycerol-repression of the methanol-utilization pathway or the lack of nitrate assimilation. Rewiring of the transcriptional networks regulating carbon (and nitrogen) source utilization clearly contributes to our understanding of genetic events occurring during evolution of yeast species. The mechanisms of mating-type switching and the triggers of morphogenic phenotypes represent further examples for how K. phaffii is distinguished from the model yeast S. cerevisiae. With respect to heterologous protein production, K. phaffii features high secretory capacity but secretes only low amounts of endogenous proteins. Different to S. cerevisiae, the Golgi apparatus of K. phaffii is stacked like in mammals. While it is tempting to speculate that Golgi architecture is correlated to the high secretion levels or the different N-glycan structures observed in K. phaffii, there is recent evidence against this. We conclude that K. phaffii is a yeast with unique features that has a lot of potential to explore both fundamental research questions and industrial applications.

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Pentose metabolism and conversion to biofuels and high-value chemicals in yeasts

Ruchała

Sibirny

2020

FEMS Microbiology Reviews

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Pentose sugars are widespread in nature and two of them, D-xylose and L-arabinose belong to the most abundant sugars being the second and third by abundance sugars in dry plant biomass (lignocellulose) and in general on planet. Therefore, it is not surprising that metabolism and bioconversion of these pentoses attract much attention. Several different pathways of D-xylose and L-arabinose catabolism in bacteria and yeasts are known. There are even more common and really ubiquitous though not so abundant pentoses, D-ribose and 2-deoxy-D-ribose, the constituents of all living cells. Thus, ribose metabolism is example of endogenous metabolism whereas metabolism of other pentoses, including xylose and L-arabinose, represents examples of the metabolism of foreign exogenous compounds which normally are not constituents of yeast cells. As a rule, pentose degradation by the wild-type strains of microorganisms does not lead to accumulation of high amounts of valuable substances; however, productive strains have been obtained by random selection and metabolic engineering. There are numerous reviews on xylose and (less) L-arabinose metabolism and conversion to high value substances; however, they mostly are devoted to bacteria or the yeast Saccharomyces cerevisiae. This review is devoted to reviewing pentose metabolism and bioconversion mostly in non-conventional yeasts, which naturally metabolize xylose. Pentose metabolism in the recombinant strains of S. cerevisiae is also considered for comparison. The available data on ribose, xylose, L-arabinose transport, metabolism, regulation of these processes, interaction with glucose catabolism and construction of the productive strains of high-value chemicals or pentose (ribose) itself are described. In addition, genome studies of the natural xylose metabolizing yeasts and available tools for their molecular research are reviewed. Metabolism of other pentoses (2-deoxyribose, D-arabinose, lyxose) is briefly reviewed.

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Carbon source requirements for mating and mating‐type switching in the methylotrophic yeasts Ogataea (Hansenula) polymorpha and Komagataella phaffii (Pichia pastoris)

Cited by 6 publications

References 38 publications

On the evolution of variation in sexual reproduction through the prism of eukaryotic microbes

On the evolution of variation in sexual reproduction through the prism of eukaryotic microbes

What makes Komagataella phaffii non-conventional?

Pentose metabolism and conversion to biofuels and high-value chemicals in yeasts

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