Carbonate hydroxyapatite functionalization: a comparative study towards (bio)molecules fixation

Russo, Laura; Taraballi, Francesca; Lupo, Cristina; Poveda, Ana; Jiménez‐Barbero, Jesús; Sandri, Monica; Tampieri, Anna; Nicotra, Francesco; Cipolla, Laura

doi:10.1098/rsfs.2013.0040

Cited by 62 publications

(38 citation statements)

References 42 publications

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“…Traditional pathogen enrichment methods are based on density gradient centrifugation and cell culture, which are still used in clinical analysis (Dorn et al, 1976;Hanff et al, 1984;Lagier et al, 2015). Several promising methods have been developed to capture pathogens in a small sample volume, such as antibody-, lectin-and antimicrobial peptide-based biological methods (Bicart-See et al, 2016;Chairatana and Nolan, 2014;Pal et al, 2017;Russo et al, 2014). A small sample amount (a few hundred microliters) is used in conventional assays bcause of their intrinsic limitations.…”

Section: Introductionmentioning

confidence: 99%

A single-tube approach for in vitro diagnostics using diatomaceous earth and optical sensor

Zhao

Koo

Liu

et al. 2018

Biosensors and Bioelectronics

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Versatile, simple and efficient sample preparation is desirable for point-of-care testing of emerging diseases such as zoonoses, but current sample preparation assays are insensitive, labour-intensive and time-consuming and require multiple instruments. We developed a single-tube sample preparation approach involving direct pathogen enrichment and extraction from human specimens using diatomaceous earth (DE). Amine-modified DE was used to directly enrich a zoonotic pathogen, Brucella, in a large sample volume. Next, a complex of amine-modified DE and dimethyl suberimidate was used for nucleic acid extraction from the enriched pathogen. Using our single-tube approach, the pathogen can be enriched and extracted within 60min at a level of 1 colony formation unit (CFU) from a 1ml sample volume in the same tube. The performance of this approach is 10-100 times better than that of a commercial kit (10 to 10CFU/ml) but does not require a large centrifuge. Finally, we combined the single-tube approach with a bio-optical sensor for rapid and accurate zoonotic pathogen detection in human urine samples. Using the combination system, Brucella in human urine can be efficiently enriched (~ 8-fold) and the detection limit is enhanced by up to 100 times (1CFU/ml bacteria in urine) compared with the commercial kit. This combined system is fast and highly sensitive and thus represents a promising approach for disease diagnosis in the clinical setting.

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Section: Introductionmentioning

confidence: 99%

A single-tube approach for in vitro diagnostics using diatomaceous earth and optical sensor

Zhao

Koo

Liu

et al. 2018

Biosensors and Bioelectronics

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“…For instance, conjugation of matricellular cues within the structure of three-dimensional scaffolds requires the definition and optimization of biofunctionalization protocols. Russo et al [5] report on a flexible and effective method for the fixation of biomolecules on scaffolds of carbonate hydroxyapatite. Following this procedure, it is virtually possible to encode any relevant biomolecular signals within materials widely used for bone tissue engineering.…”

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confidence: 99%

Nano-engineered bioactive interfaces

Netti

2014

Interface Focus.

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“…Small organic molecules with ionisable functional groups such as silanols, carboxylic acids, amines, thiols or nucleotide strands display high affinity for the CaP surface [221][222][223], and can therefore be employed as ligands. When the ligands are attached to CaP NPs, their exposed functional groups can be further conjugated with the active targeting molecules.…”

Section: Applications As Nano-carriermentioning

confidence: 99%

Calcium phosphate-based nanosystems for advanced targeted nanomedicine

Esposti

Carella

Adamiano

et al. 2018

Drug Development and Industrial Pharmacy

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Synthetic calcium phosphates (CaPs) are the most widely accepted bioceramics for the repair and reconstruction of bone tissue defects. The recent advancements in materials science have prompted a rapid progress in the preparation of CaPs with nanometric dimensions, tailored surface characteristics, and colloidal stability opening new perspectives in their use for applications not strictly related to bone. In particular, the employment of CaPs nanoparticles as carriers of therapeutic and imaging agents has recently raised great interest in nanomedicine. CaPs nanoparticles, as well as other kinds of nanoparticles, can be engineered to specifically target the site of the disease (cells or organs), thus minimizing their dispersion in the body and undesired organism-nanoparticles interactions. The most promising and efficient approach to improve their specificity is the 'active targeting', where nanoparticles are conjugated with a targeting moiety able to recognize and bind with high efficacy and selectivity to receptors that are highly expressed only in the therapeutic site. The aim of this review is to give an overview on advanced targeted nanomedicine with a focus on the most recent reports on CaP nanoparticles-based systems, specifically designed for the active targeting. The distinctive characteristics of CaP nanoparticles with respect to the other kinds of nanomaterials used in nanomedicine are also discussed.

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Carbonate hydroxyapatite functionalization: a comparative study towards (bio)molecules fixation

Cited by 62 publications

References 42 publications

A single-tube approach for in vitro diagnostics using diatomaceous earth and optical sensor

A single-tube approach for in vitro diagnostics using diatomaceous earth and optical sensor

Nano-engineered bioactive interfaces

Calcium phosphate-based nanosystems for advanced targeted nanomedicine

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