Nano-engineered bioactive interfaces
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Netti, Paolo A.

doi:10.1098/rsfs.2013.0065

Cited by 4 publications

(4 citation statements)

References 9 publications

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“…The CM-Si/ PSi bio-interface caused a signicant increase in the number of cells with nuclear Runx2/VDR co-localization and an improvement on PSi containing track areas. These results t well with previous works 4,32,33 and add a new example to support the important role of physical properties, such as roughness and stiffness of a biomaterial, in allowing progenitor cells to realize two different processes: proliferation/migration and differentiation. Thus, much work needs to be done to improve scaffolds supporting specic uid dynamics, adequate stiffness and roughness to mimic the desired tissue by directing the scaffold function with bio-interfaces, such as those exemplied herein.…”

Section: -31supporting

confidence: 73%

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Conditioned bio-interfaces of silicon/porous silicon micro-patterns lead to the chondrogenesis of hMSCs

et al. 2015

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aThe interaction between cells and materials is of scientific and technological interest for the development of new biomaterials with improved functional properties. In this work the chondrogenesis (CG) of human mesenchymal stem cells (hMSCs) derived from the bone marrow of healthy donors has been achieved on Si/PSi surfaces after pre-treatment with a conditioned medium derived from hMSCs (CM). The chondrogenic process was analyzed in fixed-cell preparations by immune fluorescence, determining the cellular localization of b-catenin and the transcription factors STAT-5, Runx2 and vitamin D receptor (VDR). In a week of CG on Si/PSi or CM-Si/PSi, b-catenin showed cell-cell contacts priming a preferential chondrogenic process on a PSi surface, in contrast with the preferential proliferation and migration processes observed on hexagonal/triangular micro-designed Si areas. Interestingly, the presence of a CM bio-interface improves this differentiation process with respect to a control PSi surface. The chondroinductive effect was also observed in STAT-5, which was highly expressed and is involved in intracellular signaling of many differentiation receptors related to development and tissue repair. In good agreement with the above results, the co-localization of transcription factors Runx2/VDR at nuclei supports active transcription of chondrogenic genes. Considering these observations, we propose that the combination of a rough PSi surface and the components of the CM-hMSCs biointerface boosts the chondrogenesis of hMSCs. These findings suggest that the tailoring of Si/PSi scaffolds with appropriate bio-interface combinations improves their therapeutic potential.

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Section: -31supporting

confidence: 73%

“…These results may be taken into account when considering new possible therapies for osteoporosis, bone distraction and cartilage or tendon-ligament fracture. 4,33,37…”

Section: Discussionmentioning

confidence: 99%

Conditioned bio-interfaces of silicon/porous silicon micro-patterns lead to the chondrogenesis of hMSCs

et al. 2015

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“…The extensive research on nano-engineered systems and polymer-based nanocomposites [7][8][9][10][11][12][13][14][15][16][17] has led to significant advances also in the field of MRI [13,[18][19][20][21][22]. In particular, some recent studies have shown that nanoconstructs can improve CAs' relaxivity and many efforts have been devoted to the & 2016 The Author(s) Published by the Royal Society.…”

Section: Introductionmentioning

confidence: 99%

“…The extensive research on nano-engineered systems and polymer-based nanocomposites [7][8][9][10][11][12][13][14][15][16][17] has led to significant advances also in the field of MRI [13,[18][19][20][21][22]. In particular, some recent studies have shown that nanoconstructs can improve CAs' relaxivity and many efforts have been devoted to the development of polymer-based carriers for MRI CAs with particular reference to Gadolinium (Gd) chelates, which are the most commonly clinically used agents [18,[23][24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Impact of biopolymer matrices on relaxometric properties of contrast agents

et al. 2016

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Properties of water molecules at the interface between contrast agents (CAs) for magnetic resonance imaging and macromolecules could have a valuable impact on the effectiveness of metal chelates. Recent studies, indeed, demonstrated that polymer architectures could influence CAs' relaxivity by modifying the correlation times of the metal chelate. However, an understanding of the physico-chemical properties of polymer/CA systems is necessary to improve the efficiency of clinically used CAs, still exhibiting low relaxivity. In this context, we investigate the impact of hyaluronic acid (HA) hydrogels on the relaxometric properties of Gd-DTPA, a clinically used CA, to understand better the determining role of the water, which is crucial for both the relaxation enhancement and the polymer conformation. To this aim, water self-diffusion coefficients, thermodynamic interactions and relaxometric properties of HA/Gd-DTPA solutions are studied through time-domain NMR relaxometry and isothermal titration calorimetry. We observed that the presence of Gd-DTPA could alter the polymer conformation and the behaviour of water molecules at the HA/Gd-DTPA interface, thus modulating the relaxivity of the system. In conclusion, the tunability of hydrogel structures could be exploited to improve magnetic properties of metal chelates, inspiring the development of new CAs as well as metallopolymer complexes with applications as sensors and memory devices.

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Multiscale Analysis of Metal Oxide Nanoparticles in Tissue: Insights into Biodistribution and Biotransformation

Matter

Leşe

et al. 2020

Advanced Science

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Metal oxide nanoparticles have emerged as exceptionally potent biomedical sensors and actuators due to their unique physicochemical features. Despite fascinating achievements, the current limited understanding of the molecular interplay between nanoparticles and the surrounding tissue remains a major obstacle in the rationalized development of nanomedicines, which is reflected in their poor clinical approval rate. This work reports on the nanoscopic characterization of inorganic nanoparticles in tissue by the example of complex metal oxide nanoparticle hybrids consisting of crystalline cerium oxide and the biodegradable ceramic bioglass. A validated analytical method based on semiquantitative X‐ray fluorescence and inductively coupled plasma spectrometry is used to assess nanoparticle biodistribution following intravenous and topical application. Then, a correlative multiscale analytical cascade based on a combination of microscopy and spectroscopy techniques shows that the topically applied hybrid nanoparticles remain at the initial site and are preferentially taken up into macrophages, form apatite on their surface, and lead to increased accumulation of lipids in their surroundings. Taken together, this work displays how modern analytical techniques can be harnessed to gain unprecedented insights into the biodistribution and biotransformation of complex inorganic nanoparticles. Such nanoscopic characterization is imperative for the rationalized engineering of safe and efficacious nanoparticle‐based systems.

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Nano-engineered bioactive interfaces

Cited by 4 publications

References 9 publications

Conditioned bio-interfaces of silicon/porous silicon micro-patterns lead to the chondrogenesis of hMSCs

Conditioned bio-interfaces of silicon/porous silicon micro-patterns lead to the chondrogenesis of hMSCs

Impact of biopolymer matrices on relaxometric properties of contrast agents

Multiscale Analysis of Metal Oxide Nanoparticles in Tissue: Insights into Biodistribution and Biotransformation

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