Carboxylated magnetic microbead-assisted fluoroimmunoassay for early biomarkers of acute myocardial infarction

Wang, Jinyi; Wang, Qiang; Ren, Li; Wang, Xueqin; Wan, Zongfang; Liu, Wenming; Li, Li; Zhao, Huiying; Li, Manlin; Tong, Dewen; Xu, Juan

doi:10.1016/j.colsurfb.2009.03.023

Cited by 17 publications

(13 citation statements)

References 47 publications

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“…Therefore, a wide variety of biomolecules including aptamers, peptides, enzymes and carbohydrates have been used to provide MNPs with specificity and bioactivity. − However, among these biomolecules, Abs present themselves as very convenient candidates due to their large diversity, high specificity, and recognition efficiency. − Several strategies have been developed for the incorporation of Abs on different supports. − It must be said though that Ab functionalization of MNPs have some limitations when compared to surfaces and microbeads, such as easy aggregation with small changes of pH and/or ionic strength, polymeric shell instability, batch to batch MNPs irreproducibility, or presence of surfactants. This can be the reason why, to our knowledge, there are in the literature several contributions regarding the complexation of magnetic microbeads with Abs, but only a few for MNPs. − One of the most popular strategies entails the covalent attachment of the Ab through their most reactive amine groups. This is a simple methodology which does not involve the chemical modification of the Ab since amine groups are present in most proteins and are very reactive moieties. − However, the use of this protocol implies working at pH values below 8.0, which results in a random orientation of the Ab on the MNP surface.…”

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confidence: 99%

Taking Advantage of Unspecific Interactions to Produce Highly Active Magnetic Nanoparticle−Antibody Conjugates

et al. 2011

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Several strategies for linking antibodies (Abs) through their Fc region in an oriented manner have been proposed at the present time. By using these strategies, the Fab region of the Ab is available for antigen molecular recognition, leading to a more efficient interaction. Most of these strategies are complex processes optimized mainly for the functionalization of surfaces or microbeads. These methodologies imply though the Ab modification through several steps of purification or the use of expensive immobilized proteins. Besides, the functionalization of magnetic nanoparticles (MNPs) turned out to be much more complex than expected due to the lack of stability of most MNPs at high ionic strength and non-neutral pH values. Therefore, there is still missing an efficient, easy and universal methodology for the immobilization of nonmodified Abs onto MNPs without involving their Fab regions during the immobilization process. Herein, we propose the functionalization of MNPs via a two-steps strategy that takes advantage of the ionic reversible interactions between the Ab and the MNP. These interactions make possible the orientation of the Ab on the MNP surface before being attached in an irreversible way via covalent bonds. Three Abs (Immunoglobulin G class) with very different isoelectric points (against peroxidase, carcinoembryonic antigen, and human chorionic gonadotropin hormone) were used to prove the general applicability of the strategy here proposed and its utility for the development of more bioactive NPs.

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confidence: 99%

Taking Advantage of Unspecific Interactions to Produce Highly Active Magnetic Nanoparticle−Antibody Conjugates

et al. 2011

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“…29,30 In this context, acrylic acid, an inexpensive substance having quite low toxicity and possessing carboxylated groups, was preferentially chosen as a functional/protective shell in the copolymeric encapsulation with styrene to form poly(styrene-acrylic acid) (PSA). 31,32 Furthermore, PSA can offer the stability to Fe 3 O 4 NPs in polar or non-polar solvent by adjusting the ratio of acrylic acid to styrene. 24,33 The determination of cholesterol concentration is important in clinical diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Covalent immobilization of cholesterol oxidase and poly(styrene-co-acrylic acid) magnetic microspheres on polyaniline films for amperometric cholesterol biosensing

et al. 2013

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Magnetic Fe 3 O 4 nanoparticles (Fe 3 O 4 NPs) were synthesized by co-precipitation (of Fe 2+ and Fe 3+ ) and surface-carboxylated by poly(styrene-co-acrylic acid) (PSA). Then they were thoroughly characterized by Scanning Electron Microscope and Transmission Electron Microscope analyses. Using glutaraldehyde cross-linking immobilization strategy, cholesterol oxidase (ChOx) was immobilized onto Fe 3 O 4 /PSA microspheres. The Fe 3 O 4 /PSA-ChOx has been successfully synthesized on the planar platinum polyaniline (PANi) modified electrode. PANi/Fe 3 O 4 /PSA-ChOx sensing properties were studied using cyclic voltammetric and chronoamperometric techniques. Under the optimized experimental conditionsthe biosensor shows excellent features, such as wide linear range, 0.2 to 1.8 mM (R 2 ¼ 0.9901), low detection limit (0.02 mM), short response time (5-10 s), and high sensitivity (8796 mA mM À1 cm À2 ). The interference test with selected organic compounds, such as glucose, ascorbic acid, uric acid, and acetaminophen indicated high selectivity of the above sensor. It can be proven that the proposed PANi/ Fe 3 O 4 /PSA-ChOx based sensing concept could be successfully used for other biodetections.

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“…However, the tests available are time consuming, expensive, and non-reliable and needs specialized protocols for sample preparation (3,5). These drawbacks can be eliminated to a certain extent with the help of microfluidics based biosensor platform.…”

Section: Introductionmentioning

confidence: 99%

Microfluidics for Detection of Myoglobin in Blood Samples

Gunda¹,

Mitra²

2011

ECS Trans.

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Carboxylated magnetic microbead-assisted fluoroimmunoassay for early biomarkers of acute myocardial infarction

Cited by 17 publications

References 47 publications

Taking Advantage of Unspecific Interactions to Produce Highly Active Magnetic Nanoparticle−Antibody Conjugates

Taking Advantage of Unspecific Interactions to Produce Highly Active Magnetic Nanoparticle−Antibody Conjugates

Covalent immobilization of cholesterol oxidase and poly(styrene-co-acrylic acid) magnetic microspheres on polyaniline films for amperometric cholesterol biosensing

Microfluidics for Detection of Myoglobin in Blood Samples

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