Carboxymethyl Dextran-Cyclodextrin Conjugate as the Carrier of Doxorubicin

Abstract

The carboxymethyl dextran-y-cyclodextrin (CMD-yCD) conjugate was prepared as the carrier for the delivery of the poorly water-soluble anticancer drug, doxorubicin (DOX). The conjugate could form self-assembled nanoparticles (315 nm in diameter) in an aqueous solution, which might be due to the hydrogen bonding among yCD molecules in the conjugate. DOX was effectively encapsulated into CMD-yCD nanoparticles (CMD-NPs) by the emulsion method. In particular, regardless of the feed amount of DOX, its loading efficiencies were always greater than 70%. CMD-NPs released DOX in a sustained manner, owing to the inclusion complex formation between DOX and yCD. When Cy5.5-labeled CMD-NPs were treated with SCC7 cancer cells, strong fluorescence signals were observed at the cytosol, indicating effective intracellular uptake. In addition, DOX-loaded CMD-NPs exhibited dose-dependent cytotoxicity to SCC7 cancer cells. However, the empty nanoparticles did not show toxicity to the cells, implying their high biocompatibility. Overall, these results suggest that the CMD-gammaCD conjugate could be a useful carrier for the delivery of DOX.

Bioreducible Carboxymethyl Dextran Nanoparticles for Tumor‐Targeted Drug Delivery

“…During the synthesis procedure, degree of substitution (DS), defined as the number of LCAs per 100 CMD sugar residues, was controlled by varying the molar feed ratio of LCA to the carboxyl group in CMD. DS was calculated based on the integration ratio of the proton peak appearing from the methyl group of LCA at 0.70 ppm (18‐CH 3 ) and that from the carboxymethylene proton of CMD at 4.02 ppm . As expected, the DS of LCA increased as the feed ratio of LCA increased ( Table 1 ).…”

Applications of Nanoparticles for Anticancer Drug Delivery: A Review

“…High aqueous solubility and the ability to encapsulate hydrophobic moieties within their cavity through the formation of inclusion complexes enable cyclodextrins to enhance the solubility, stability, and bioavailability of hydrophobic small-molecule drugs. 319 320 Davis et al reported a cyclodextrin polymer-based nanoparticle delivery system for the systemic administration of siRNA to patients with solid tumors. 321 RNA interference was used to reduce the expression of M2-subunit of ribonucleotide reductase (RRM2).…”

“…Abbasi et al prepared â-cyclodextrin-helenalin complexes and showed that â-cyclodextrin-helenalin complexes inhibited the growth of the T47D breast cancer cell line. 324 Cyclodextrin could also be used to deliver the poorly water-soluble anticancer drug, doxorubicin. 325 …”

Polyurethane foam containing rhEGF as a dressing material for healing diabetic wounds: Synthesis, characterization, in vitro and in vivo studies

“…Hydrophilic PUs have several strengths, including maintenance of a moist environment, high absorbency for exudates and high moisture permeability. In addition to the synthetic polymers, natural biopolymers including hyaluronic acid, chitosan, and dextran derivatives have been extensively investigated in biomedical applications mainly because of their biocompatibility, non-immunogenicity, biodegradability and cell-adhesion properties [3,[29][30][31][32][33][34]. Among the variety of natural polysaccharides, chitosan is a positively charged linear polysaccharide composed of glucosamine and N-acetyl glucosamine derivatives, has been widely used as a wound dressing material [30].…”