Carboxymethylcellulose–gelatin–superoxidase dismutase electrode for amperometric superoxide radical sensing

Abstract

A novel, highly sensitive superoxide dismutase biosensor for the direct and simultaneous determination of superoxide radicals was developed by immobilization of superoxide dismutase within carboxymethylcellulose-gelatin on a Pt electrode surface. The parameters affecting the performance of the biosensor were investigated. The response of the CMC-G-SOD biosensor was proportional to O (2) (·-) concentration and the detection limit was 1.25 × 10(-3) mM with a correlation coefficient of 0.9994. The developed biosensor exhibited high analytical performance with wider linear range, high sensitivity and low response time. The biosensor retained 89.8% of its sensitivity after use for 80 days. The support system enhanced the immobilization of superoxide dismutase and promoted the electron transfer of superoxide dismutase minimizing its fouling effect. The biosensor was quite effective not only in detecting O (2) (·-) , but also in determining the antioxidant properties of acetylsalicylic acid-based drugs and the anti-radical activity of healthy and cancerous human brain tissues.

Biomimetic Superoxide Dismutase Stabilized by Photopolymerization for Superoxide Anions Biosensing and Cell Monitoring

“…The linear range was wider than those of some other modified electrodes ,− (Table S1 in Supporting Information). The detection limit was estimated to be 0.029 μmol L –1 at a signal-to-noise ratio of 3:1, which was lower than those of some other modified electrodes and methods − (Table S2 in Supporting Information). The normal level of O 2 •– in physiological condition is about 0.1 μmmol L –1 and its concentration increases under traumatic brain injury ischemia-reperfusion, hypoxia, and other oxidative stress diseases. , Thus, the proposed amperometric biosensor showed promising application in the monitoring of O 2 •– with high sensitivity and wide concentration range due to the high efficient catalysis of Mn 2 P 2 O 7 and fast interfacial electron transfer at the surface of MWCNTs/Mn 2 P 2 O 7 –formylstyrylpyridine modified films.…”

Recent Developments of Carboxymethyl Cellulose

“…Recently, Cui et al (2019) [322] used a reduced graphene oxide/CMC blend to modify the glassy carbon electrodes of an electrochemical biosensor to detect vitamin B 6 . Kocabay et al (2012) [323] developed a novel CMC/gelatin/superoxide dismutase-based biosensor to see the number of superoxide radicals (O 2 • − ), which is considered as the primary species of ROS (i.e., reactive oxygen species) that can bring various types of reversible or permanent damages to several biomolecules. The biosensor exhibited high accuracy (e.g., correlation coefficient, R 2 = 0.9994) and high sensitivity toward the targeted species, even at a low detection limit (i.e., 1.25 × 10 −3 mM).…”

Applications of Gelatin in Biosensors: Recent Trends and Progress

“…Biosensors with gelatin as matrix for immobilized biorecognition materials including several additional works [ 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 ] are summarized in Table 1 .…”