Carcinoembryonic antigen and cytokeratin 20 in peritoneal cells of cancer patients: are we aware of what we are detecting by mRNA examination?

Kowalewska, Magdalena; Chechlińska, Magdalena; Nowak, Radosława

doi:10.1038/sj.bjc.6604189

Cited by 17 publications

(14 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some criticism have been moved to this molecular technique, since some Authors believe that the expression of some genes used for the identification of tumor cells may be present in inflammatory cells as well, resulting real time qRT‐PCR in a high sensitivity and low specificity test 20.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Free peritoneal tumor cells detection in gastric and colorectal cancer patients

Monte¹,

Ranieri²,

Mazzetta³

et al. 2012

Journal of Surgical Oncology

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

“…In particular, CEA and CK20 evaluated with multivariate analysis represent independent prognostic markers 19. However, it has been also argued that the detection of these markers by RT‐PCR‐based methods is of limited value because both CEA and CK20 can be expressed and released by hematopoietic cells in the inflammatory context 20.…”

Section: Introductionmentioning

confidence: 99%

Free peritoneal tumor cells detection in gastric and colorectal cancer patients

Monte¹,

Ranieri²,

Mazzetta³

et al. 2012

Journal of Surgical Oncology

View full text Add to dashboard Cite

show abstract

“…However, their significance as a prognostic factor for survival or a predictive factor for peritoneal recurrence is not clear [ 14 ]. ICC is subjective and depends on the strength of cellular staining, and PCR-based methods have inherent problems as they detect RNA, not viable cells, and cannot delineate cancerous cells from nonmalignant cells or cellular debris [ 13 , 15 ]. In addition, the targeted antigen or RNAs are heterogeneous between studies [ 15 , 16 , 17 , 18 , 19 ].…”

Section: Discussionmentioning

confidence: 99%

Role of Peritoneal Lavage Cytology and Prediction of Prognosis and Peritoneal Recurrence After Curative Surgery for Colorectal Cancer

Bae

Shin

et al. 2014

Ann Coloproctol

View full text Add to dashboard Cite

PurposeIn colorectal cancer, the role of detecting free malignant cells from peritoneal lavage is currently unclear. In this study, we investigated the positive rate of free malignant cells in peritoneal lavage fluid and their predictive value for prognosis and peritoneal recurrence after a curative resection.MethodsFrom October 2009 to December 2011, in a prospective manner, we performed cytologic examinations of peritoneal lavage fluid obtained just after the abdominal incision from 145 patients who underwent curative surgery for colorectal cancer. We used proportional hazard regression models to analyze the predictive role of positive cytology for peritoneal recurrence and survival.ResultsAmong total 145 patients, six patients (4.1%) showed positive cytology. During the median follow-up of 32 months (range, 8-49 months), 27 patients (18.6%) developed recurrence. Among them, 5 patients (3.4%) showed peritoneal carcinomatosis. In the multivariate analysis, positive cytology was an independent predictive factor for peritoneal recurrence (hazard ratio [HR], 136.5; 95% confidence interval [CI], 12.2-1,531.9; P < 0.0001) and an independent poor prognostic factor for overall survival (HR, 11.4; 95% CI, 1.8-72.0; P = 0.009) and for disease-free survival (HR, 11.1; 95% CI, 3.4-35.8; P < 0.0001).ConclusionPositive cytology of peritoneal fluid was significantly associated with peritoneal recurrence and worse survival in patients undergoing curative surgery for colorectal cancer. Peritoneal cytology might be a useful tool for selecting patients who need intraperitoneal or systemic chemotherapy.

show abstract

“…However, ICC (along with other histological staining techniques) is subjective and depends on the strength of cellular staining. PCR‐based methods have inherent problems as they detect DNA, not viable cells, and cannot delineate cancerous cells from non‐malignant cells or cellular debris. These factors may explain the marked difference in yield rates, and the positive results seen in negative controls.…”

Section: Discussionmentioning

confidence: 99%