Cardamonin inhibits nitric oxide production modulated through NMDA receptor in LPS-induced SH-SY5Y cell in vitro model

Kaswan, Nur Khalisah; Suhaimi, Nurul Syazwani Mohd; Izham, Noor Aishah Mohammed; Mohamad, Tengku Azam Shah Tengku; Sulaiman, Mohd Roslan; Perimal, Enoch Kumar

doi:10.28916/lsmb.4.9.2020.58

Cited by 3 publications

(3 citation statements)

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“…TRPv1 and NMDA N2B receptors are the leading player underlying hyperalgesia and allodynia in neuropathic pain conditions (Carrasco et al, 2018;Chia et al, 2020). In addition, LPS could also induce changes in reactive oxidative stress (ROS), a similar condition observed in chemotherapy-induced and diabetic-induced neuropathic pain, as previously discussed (Chanchal et al, 2016;Kaswan et al, 2020). The utilisation of LPS in inducing neuropathic pain pathophysiology in vitro model has been considered comprehensive as it can induce an inflammatory response and alters the leading players of nociceptive pathways such as the ion channels and receptors.…”

Section: Lipopolysaccharides-induced Neuropathic Pain Conditionmentioning

confidence: 78%

“…Furthermore, the induction of neuropathic pain-like condition on SH-SY5Y cell culture has resulted in changes in the expression of inflammatory proteins and signalling molecules, namely TLR receptors, interleukins, NFκB and nitric oxide (Amine et al, 2021;Kaswan et al, 2020;Lawrimore & Crews, 2017). SH-SY5Y cells are also commonly co-cultured with microglial cell lines to study the neuron-glial interaction in chronic pain mechanisms (Anand et al, 2015;Pandur et al, 2018).…”

Section: Sh-sy5y Cellsmentioning

confidence: 99%

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Exploring the possibilities of using in vitro model for neuropathic pain studies

et al. 2022

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Establishing experimental models to study neuropathic pain has been challenging due to the complex mechanism underlying the condition. Although in vivo models have been useful in the observation of behavioural pain responses, it should be acknowledged that species-to-species variance can lead to differences in terms of molecular mechanism and genetic expression. The study of molecular and signal transduction of neuropathic pain using in vivo models faces limitations due to ethical considerations involving pain induction in animals and the intricacy of molecular interactions in the pathophysiology of the condition. Hence, developing relevant in vitro models to study neuropathic pain is important, as it considers the physiological microenvironment and reduces the use of experimental animals. Several considerations should be taken into account in developing an in vitro model of neuropathic pain, including the use of either primary culture of cell lines with considerations to their origins; human or animal, the method of neuropathic pain-like induction and the relevant assays to assess pain. This review recapitulates previous research employing in vitro models in investigating the molecular mechanism of neuropathic pain, intending to provide an alternative to the growing concerns on in vivo neuropathic pain models.

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Section: Lipopolysaccharides-induced Neuropathic Pain Conditionmentioning

confidence: 78%

Section: Sh-sy5y Cellsmentioning

confidence: 99%

Exploring the possibilities of using in vitro model for neuropathic pain studies

et al. 2022

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“…The multitargeting properties of cardamonin is the key to its therapeutic effects against chronic diseases. Previous studies have demonstrated that cardamonin elicits antihyperalgesic and antiallodynic effects in CCI-induced neuropathic pain mice model modulated via the opioidergic and glutamatergic pathways [ 11 , 47 , 48 ]. With this understanding, we are now further investigating the possible mechanism of action of cardamonin in neuropathic pain by evaluating the involvement of the serotonergic pathways in cardamonin’s ability to exert antihyperalgesic and antiallodynic properties.…”

Section: Discussionmentioning

confidence: 99%

Cardamonin Modulates Neuropathic Pain through the Possible Involvement of Serotonergic 5-HT1A Receptor Pathway in CCI-Induced Neuropathic Pain Mice Model

et al. 2021

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Cardamonin, a naturally occurring chalcone isolated from Alpinia species has shown to possess strong anti-inflammatory and anti-nociceptive activities. Previous studies have demonstrated that cardamonin exerts antihyperalgesic and antiallodynic properties in chronic constriction injury (CCI)-induced neuropathic pain animal model. However, the mechanisms underlying cardamonin’s effect have yet to be fully understood. The present study aims to investigate the involvement of the serotonergic system in cardamonin induced antihyperalgesic and antiallodynic effects in CCI-induced neuropathic pain mice model. The neuropathic pain symptoms in the CCI mice model were assessed using Hargreaves Plantar test and von-Frey filament test on day 14 post-surgery. Central depletion of serotonin along the descending serotonergic pathway was done using ρ-chlorophenylalanine (PCPA, 100 mg/kg, i.p.), an inhibitor of serotonin synthesis for four consecutive days before cardamonin treatment, and was found to reverse the antihyperalgesic and antiallodynic effect produced by cardamonin. Pretreatment of the mice with several 5-HT receptor subtypes antagonists: methiothepin (5-HT1/6/77 receptor antagonist, 0.1 mg/kg), WAY 100635 (5-HT1A receptor antagonist, 1 mg/kg), isamoltane (5-HT1B receptor antagonist, 2.5 mg/kg), ketanserin (5-HT2A receptor antagonist, 0.3 mg/kg), and ondansetron (5-HT3 receptor antagonist, 0.5 mg/kg) were shown to abolish the effect of cardamonin induced antihyperalgesic and antiallodynic effects. Further evaluation of the 5-HT1A receptor subtype protein expressions reveals that cardamonin significantly upregulated its expression in the brainstem and spinal cord. Our results suggest that the serotonergic pathway is essential for cardamonin to exert its antineuropathic effect in CCI mice through the involvement of the 5-HT1A receptor subtype in the central nervous system.

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A Comprehensive Review on the Anti-inflammatory Activity of Chalconesderived Moieties

Mittal

Sharma²,

Kushwah³

et al. 2024

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Background: In recent decades, all-embracing research accounting for thousands of natural bioactive compounds have been detailed on behalf of their origin, pharmacological activity, active phytoconstituents, and therapeutic applications. Chalcones, secondary metabolites have shown therapeutic potential against various forms of inflammation, pain, and cancer in multiple research papers targeting their pathway inhibition and their significant therapeutic activity. Area Covered: Diverse aspects of chalcones have been focused on with their biological source, pathway inhibition, and pharmacological activity. Objectives: This study aims to focus on the chalcones being the origin of ethnopharmacological, possess extensive pharmacological activities, including anti-neoplastic, antimicrobial, anti-inflammatory, antioxidant, anti-acne, anti-aging, hepatoprotective, neuroprotective, psychoactive, anti-parasitic, and many others. Method: Chalcones display anti-inflammatory activity via COX-2, NO synthase, and TNF-α inhibition. Furthermore, comprehensive studies will be discussed with a viewpoint of the chalcones’s role in the alleviation of inflammation. According to recent advancements, chalcones clinically have been used in the treatment of inflammation, anti-aging, oxidative stress reduction, autophagy, and apoptosis Conclusion: This article focuses on the molecular progression of inflammation and the chalcone role in combating inflammation by targeting inflammatory mediators and pathways.

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Cardamonin inhibits nitric oxide production modulated through NMDA receptor in LPS-induced SH-SY5Y cell in vitro model

Cited by 3 publications

References 67 publications

Exploring the possibilities of using in vitro model for neuropathic pain studies

Exploring the possibilities of using in vitro model for neuropathic pain studies

Cardamonin Modulates Neuropathic Pain through the Possible Involvement of Serotonergic 5-HT1A Receptor Pathway in CCI-Induced Neuropathic Pain Mice Model

A Comprehensive Review on the Anti-inflammatory Activity of Chalconesderived Moieties

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