Cardiac expression of polysialylated NCAM in the chicken embryo: Correlation with the ventricular conduction system

Watanabe, Michiko; Timm, Maureen; Fallah‐Najmabadi, Hessam

doi:10.1002/aja.1001940206

Cited by 36 publications

(18 citation statements)

References 65 publications

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“…Specificity of staining was verified by the absence of stain ing in tissue sections in which PSA was removed by incu bation with endo-N or when the antibody was preincu bated with chicken PSA-NCAM. Moreover, immunoblot analyses of extract from the SCN region (and other brain areas) revealed an immunoreactive band migrating in the 150-to 300-kD range, which is characteristic of PSA staining seen in different species [15,29,31,37,38]. These results are also consistent with a previous observa tion of immunostaining for PSA in the rat SCN [15].…”

Section: Discussionsupporting

confidence: 81%

Expression of Immunoreactive Polysialylated Neural Cell Adhesion Molecule in the Suprachiasmatic Nucleus

et al. 1994

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Light-microscopic and immunoblot immunochemical procedures were used to study the distribution and biochemical characteristics of neural cell adhesion molecule (NCAM) and its polysialylated form (PSA-NCAM) in the suprachiasmatic nuclei (SCN) of the adult Siberian hamster. In the adult brain PSA-NCAM is located in regions capable of undergoing morphological rearrangements and thus is generally considered to be an indicator of neural plasticity. Immunostaining for PSA in the Siberian hamster SCN (using a monoclonal antibody against the α2,8-linked PSA of NCAM) was evident throughout the rostrocaudal axis of the SCN, with the most intense reaction in the ventrolater-al region. Immunoreactivity was present in the neuropil, which delineated groups of cells with unstained cytoplasm. Many of the SCN cells were aggregated into cords or clusters. The optic chiasm was free from label, except for short processes apparently extending from the densely stained neuropil in the ventrolateral SCN. Immunoreactivity was abolished by preincubating sections in an endoneuraminidase (endo-N) or by preincubation of the primary antibody with PSA-NCAM. Immunostaining of the nonsialylated NCAM polypeptide was also limited to the neuropil, but this was more diffuse and less regionally specific than PSA staining. The ventral SCN exhibited the most intense labeling for NCAM. Immunoblot analyses revealed the immunoreactive PSA-NCAM as a broad band migrating between apparent molecular weights in the range of 150-300 kD, which was ablated by treatment of SCN tissue extract with endo-N. This electrophoretic migration pattern of PSA-NCAM from the SCN region was similar to that seen in several other brain regions. The immunoreactive NCAM polypeptides appeared as three major bands, at apparent molecular weights of 120, 140 and 180 kD. Removal of PSA groups from PSA-NCAM with endo-N markedly increased the intensity of the 180-kD and, to a lesser extent, the 140-kD bands, implicating these NCAM isoforms as carriers of PSA. These results confirm the expression of PSA-NCAM in the adult SCN, which is evidence that cellular elements of the circadian pacemaker system may undergo morphological rearrangements.

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Section: Discussionsupporting

confidence: 81%

Expression of Immunoreactive Polysialylated Neural Cell Adhesion Molecule in the Suprachiasmatic Nucleus

et al. 1994

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“…Assuming that the innervatinn of the heart is similar to the brain in not expressing forms of NCAM recognized by anti-MSD antibodies, then the data would suggest that at least the 185-kD form of NCAM is produced within the heart. It is tempting to speculate that the ld form of NCAM may be preferentially expressed in the conduction system, given that the polysialic moieties found on NCAM, as well as the carbohydrate epitopos recognized by monoclonal antibody HNK-1 and found on NCAM and other neural adhesion molecules, are also preferentially expressed in this region of the heart (Watanabe et al, 1992;Nakagawa et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

NCAM polypeptides in heart development: association with Z discs of forms that contain the muscle-specific domain.

Byeon

Sugi

Markwald

et al. 1995

The Journal of Cell Biology

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Abstract. Previous studies of neural cell adhesion molecule (NCAM) cDNAs have revealed an alternatively spliced set of small exons (12A, 12B, 12C, and 12D) that encode a region in the extracellular portion of the molecule known as the muscle-specific domain (MSD). The entire MSD region can be expressed in skeletal muscle, heart, and skin; only exons 12A and 12D have been found in brain. These studies did not reveal which NCAM polypeptides contain the MSD region or the immunohistochemical distribution of these NCAM molecules. To address these questions, we prepared antibodies against the oligopeptides encoded by exons 12A and 12B and by exons 12C and 12D, and we used these antibodies to study the forms of NCAM containing the MSD region expressed during embryonic chicken heart development. These antibodies recognize certain forms of NCAM found in the heart, but they do not recognize brain NCAM. In the heart, each of the splice variants of NCAM (large cytoplasmic domain, small cytoplasmic domain, and small surface domain) that differ in their mode of attachment to the plasma membrane or in the size of their cytoplasmic domain is expressed in a form that contains and in a form that lacks the MSD region. No microheterogeneity is observed in the size of NCAM molecules containing the MSD region, even at the level of cyanogen bromide fragments, suggesting that exons 12A-D are expressed as a single unit. Depending on the site and the stage of development, the percent of NCAM molecules containing the MSD region can vary from nearly 0 to 100%. In general, this percentage increases during development. In immunohistochemical studies of hearts from stage 18 embryos, forms of NCAM containing the MSD region colocalized with Z discs. No other adhesion molecules were found in this distribution at this early stage of development. Studies on isolated cells in vitro demonstrate that the colocalization with Z discs of NCAM molecules containing the MSD region does not depend on cell-cell contact, and they raise the possibility that this form of NCAM is involved in cell-extracellular matrix interactions. The association of NCAM molecules containing the MSD region with Z discs suggests that this form of NCAM is involved in early myofibrillogenesis.

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“…Cell adhesion molecules showing homophilic interactions might play an important role in this process. The neural cell adhesion molecule (N-CAM), for example, has been found in the developing epicardium as well as in the myocardium [Burroughs et al, 1991;Lackie et al, 1991;Watanabe et al, 1992]. N-CAM knockout mice, however, apparently show no anomalies in the epicardium [Cremer et al, 1997].…”

Section: Cells From the Epicardial Primordium Are Transferred Across mentioning

confidence: 99%

The Origin, Formation and Developmental Significance of the Epicardium: A Review

Männer

Pérez‐Pomares

Macías

et al. 2001

Cells Tissues Organs

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250

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Questions on the embryonic origin and developmental significance of the epicardium did not receive much recognition for more than a century. It was generally thought that the epicardium was derived from the outermost layer of the primitive myocardium of the early embryonic heart tube. During the past few years, however, there has been an increasing interest in the development of the epicardium. This was caused by a series of new embryological data. The first data showed that the epicardium did not derive from the primitive myocardium but from a primarily extracardiac primordium, called the proepicardial serosa. Subsequent data then suggested that the proepicardial serosa and the newly formed epicardium provided nearly all cellular elements of the subepicardial and intermyocardial connective tissue, and of the coronary vasculature. Recent data even suggest important modulatory roles of the epicardium and of other proepicardium-derived cells in the differentiation of the embryonic myocardium and cardiac conduction system. The present paper reviews our current knowledge on the origin and embryonic development of the epicardium.

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Cardiac expression of polysialylated NCAM in the chicken embryo: Correlation with the ventricular conduction system

Cited by 36 publications

References 65 publications

Expression of Immunoreactive Polysialylated Neural Cell Adhesion Molecule in the Suprachiasmatic Nucleus

Expression of Immunoreactive Polysialylated Neural Cell Adhesion Molecule in the Suprachiasmatic Nucleus

NCAM polypeptides in heart development: association with Z discs of forms that contain the muscle-specific domain.

The Origin, Formation and Developmental Significance of the Epicardium: A Review

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