Cardiac outflow tract malformations in chick embryos exposed to homocysteine

Boot, Marit J.; Steegers‐Theunissen, R.P.M.; Poelmann, Robert E.; Iperen, Liesbeth van; Groot, Adriana C. Gittenberger‐de

doi:10.1016/j.cardiores.2004.07.010

Cited by 58 publications

(62 citation statements)

References 35 publications

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“…The significant correlation between dietary vitamin B 12 intake and the corresponding biomarker supports this finding. Moreover, experimental studies substantiate the finding of the teratogenic effect of mild hyperhomocysteinemia [33]. Our data suggest that hyperhomocysteinemia in the subgroup of case-mothers can partly be explained by the significantly lower vitamin B 6 intake and low folate and vitamin B 12 status.…”

Section: Resultssupporting

confidence: 85%

Dietary intake of B-vitamins in mothers born a child with a congenital heart defect

et al. 2006

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Section: Resultssupporting

confidence: 85%

Dietary intake of B-vitamins in mothers born a child with a congenital heart defect

et al. 2006

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“…Some studies have used L-Hcysthiolactone rather than the thiol form of D,L-Hcys because of its greater stability and because the thiol form of Hcys is only available as a D,L-isomer mixture (Rosenquist et al, 1996;Epeldegui et al, 2002;Boot et al, 2004a). However, thiolactone alone can have biological activities independent from the thiol form of Hcys (Jakubowski, 1997;Jakubowski et al, 2000).…”

Section: Effect Of Homocysteine On Cardiac Nc Cell Attachmentmentioning

confidence: 99%

Homocysteine enhances cardiac neural crest cell attachment in vitro by increasing intracellular calcium levels

Heidenreich

Reedy

Brauer

2008

Developmental Dynamics

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Elevated homocysteine (Hcys) increases the risk of neurocristopathies. Previous studies show Hcys inhibits neural crest (NC) cell migration in vivo. However, the mechanisms responsible for this effect are unknown. Here, we evaluated the effect of Hcys on NC cell attachment in vitro and determined if any of the effects were due to altered Ca 2؉ signaling. We found Hcys enhanced NC cell attachment in a dose and substratedependent manner. Ionomycin mimicked the effect of Hcys while BAPTA-AM and 2-APB blocked the effect of Hcys on NC attachment. In contrast, inhibitors of plasma membrane Ca 2؉ channels had no effect on NC attachment. Hcys also increased the emission of the intracellular Ca 2؉ -sensitive probe, Fluo-4. These results show Hcys alters NC attachment by triggering an increase in intracellular Ca 2؉ possibly by generating inositol triphosphate. Hence, the teratogenic effect ascribed to Hcys may be due to perturbation of intracellular Ca 2؉ signaling.

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“…Grupę kontrolną (GK) stanowiły 54 pary matek i ich zdrowe dzieci. Próbki krwi matki i krwi pępowinowej pobierano w celu oznaczenia stężenia tHcy, kwasu foliowego i witaminy B 12 . W Y N I K I : Stężenie tHcy u noworodków z WWS było znamiennie wyższe niż u ich matek oraz u dzieci z WCN.…”

Section: A T E R I a ł I M E T O D Yunclassified

Homocysteine remethylation pathway in neonates with congenital heart disease and neural tube defects

Surmiak

Baumert

Walencka

et al. 2018

Ann. Acad. Med. Siles.

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AB ST R ACT I N T R O D U C T I O N :The etiology of congenital heart defects (CHD) and neural tube defects (NTD) remain unknown, however, the relation between homocysteine and folate levels and congenital anomalies were found. With this perspective in mind, the aim of the study was to investigate serum biomarkers of the homocysteine metabolism pathway in neonates with CHD, newborns with NTD and their mothers. M A T E R I A L S A N D M E T H O D S :Twenty-nine pairs of mothers and their neonates with CHD as well as 18 pairs of mothers and neonates with NTD were enrolled in the study. The control group consisted of 54 pairs of mothers and their healthy neonates. To estimate the total homocysteine, serum folic acid and vitamin B 12 levels in plasma, mothers' venous blood samples and umbilical cord blood were taken in the all groups. R E S U L T S : There were significantly higher tHcy levels in the newborns with CHD compared to their mothers. The total homocysteine levels in the CHD neonates were noticeably different compared to the neonates with NTD and to the controls. The vitamin B 12 levels were similar in all the investigated neonates. Significantly lower umbilical folic acid levels in the NTD and CHD groups as compared to the controls were noticed. C O N C L U S I O N S :The observed differences in concentrations of homocysteine, folic acid and cobalamin between neonates with congenital heart and neural tube defects suggest the influence of various agents disturbing the homocysteine metabolic pathways in those children. K E Y W O R D Shomocysteine, neural tube defect, congenital heart disease, folate, cobalamin

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Cardiac outflow tract malformations in chick embryos exposed to homocysteine

Abstract: This study demonstrates that homocysteine disturbs apoptosis and myocardialization of the outflow tract, probably by affecting the cardiac neural crest cells.

Cited by 58 publications

References 35 publications

Dietary intake of B-vitamins in mothers born a child with a congenital heart defect

Dietary intake of B-vitamins in mothers born a child with a congenital heart defect

Homocysteine enhances cardiac neural crest cell attachment in vitro by increasing intracellular calcium levels

Homocysteine remethylation pathway in neonates with congenital heart disease and neural tube defects

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