Cardiac screening of intact Drosophila melanogaster larvae under exposure to aqueous and gaseous toxins in a microfluidic device

Ardeshiri, Ramtin; Hosseini, Leya; Amini, N.; Rezai, Pouya

doi:10.1039/c6ra14159e

Cited by 11 publications

(4 citation statements)

References 43 publications

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“…However, immobilization of larvae is difficult because of its digging and burrowing motion. Traditional immobilization techniques, such as tape or glue, still allow minor larval movement and reduce larval viability [49, 50]. Therefore, several strategies have been developed to immobilize samples.…”

Section: Microfluidic Devices Enable Controlled Imaging and Perturbatmentioning

confidence: 99%

“…Orienting a multicellular sample during loading is a frequently encountered problem. To overcome this, Ardeshiri et al employed a rotatable glass that can suck onto the head of the larva to rotate the larva [49, 58]. Another creative solution allows samples to be prepared on the cover glass first before the silicone slab is placed on top to form the channels of the device [59].…”

Section: Microfluidic Devices Enable Controlled Imaging and Perturbatmentioning

confidence: 99%

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Tools to reverse-engineer multicellular systems: case studies using the fruit fly

Kumar

Velagala

et al. 2019

J Biol Eng

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Reverse-engineering how complex multicellular systems develop and function is a grand challenge for systems bioengineers. This challenge has motivated the creation of a suite of bioengineering tools to develop increasingly quantitative descriptions of multicellular systems. Here, we survey a selection of these tools including microfluidic devices, imaging and computer vision techniques. We provide a selected overview of the emerging cross-talk between engineering methods and quantitative investigations within developmental biology. In particular, the review highlights selected recent examples from the Drosophila system, an excellent platform for understanding the interplay between genetics and biophysics. In sum, the integrative approaches that combine multiple advances in these fields are increasingly necessary to enable a deeper understanding of how to analyze both natural and synthetic multicellular systems.

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Section: Microfluidic Devices Enable Controlled Imaging and Perturbatmentioning

confidence: 99%

Section: Microfluidic Devices Enable Controlled Imaging and Perturbatmentioning

confidence: 99%

Tools to reverse-engineer multicellular systems: case studies using the fruit fly

Kumar

Velagala

et al. 2019

J Biol Eng

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show abstract

“…Microfluidic technology has recently emerged as a powerful tool for neural and behavioral investigation of small model organisms such as C. elegans, [35][36][37][38][39][40][41][42] D. melanogaster, [43][44][45][46] and D. rerio. [47][48][49][50][51][52][53] Most recently, various microfluidic techniques have been introduced for precise manipulation, stimulation, and monitoring of zebrafish larvae's behavior.…”

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confidence: 99%

A microfluidic device to study electrotaxis and dopaminergic system of zebrafish larvae

2018

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The zebrafish is a lower vertebrate model organism offering multiple applications for both fundamental and biomedical research into the nervous system from genes to behaviour. Investigation of zebrafish larvae's movement in response to various stimuli, which involves the dopaminergic system, is of interest in the field of sensory-motor integration. Nevertheless, the conventional methods of movement screening in Petri dishes and multi-well plates are mostly qualitative, uncontrollable, and inaccurate in terms of stimulus delivery and response analysis. We recently presented a microfluidic device built as a versatile platform for fluid flow stimulation and high speed time-lapse imaging of rheotaxis behaviour of zebrafish larvae. Here, we describe for the first time that this microfluidic device can also be used to test zebrafish larvae's sense of the electric field and electrotaxis in a systemic manner. We further show that electrotaxis is correlated with the dopamine signalling pathway in a time of day dependent manner and by selectively involving the D2-like dopamine receptors. The primary outcomes of this research opens avenues to study the molecular and physiological basis of electrotaxis, the effects of known agonist and antagonist compounds on the dopaminergic system, and the screen of novel pharmacological tools in the context of neurodegenerative disorders. We propose that this microfluidic device has broad application potential, including the investigation of complex stimuli, biological pathways, behaviors, and brain disorders.

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“…Microfluidic lab-on-chip devices are increasingly being used for studying model organisms such as Caenorhabditis elegans [8][9][10] and Drosophila. [11][12][13][14] These devices have the ability to automate immobilization of these small organisms and can be applied for in vivo visualization and tracking of cellular and physiological responses. Microfluidic-based immobilization techniques for C. elegans have been well developed using chemical (CO 2 ) or mechanical [tapering microchannels or encapsulation using deflectable polydimethylsiloxane (PDMS) membranes] approaches.…”

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confidence: 99%

Characterization of microfluidic clamps for immobilizing and imaging of Drosophila melanogaster larva's central nervous system

et al. 2017

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is a well-established model organism to understand biological processes and study human diseases at the molecular-genetic level. The central nervous system (CNS) of larvae is widely used as a model to study neuron development and network formation. This has been achieved by using various genetic manipulation tools such as microinjection to knock down certain genes or over-express proteins for visualizing the cellular activities. However, visualization of an intact-live neuronal response in larva's Central Nervous System (CNS) is challenging due to robust digging/burrowing behaviour that impedes neuroimaging. To address this problem, dissection is used to isolate and immobilize the CNS from the rest of the body. In order to obtain a true physiological response from the CNS, it is important to avoid dissection, while the larva should be kept immobilized. In this paper, a series of microfluidic clamps were investigated for intact immobilization of the larva. As a result, an optimized structure for rapid mechanical immobilization of larvae for CNS imaging was determined. The clamping and immobilization processes were characterized by imaging and movement measurement of the CNS through the expression of genetically encoded Calcium sensor GCaMP5 in all sensory and cholinergic interneurons. The optimal structure that included two 3D constrictions inside a narrowed channel considerably reduced the internal CNS capsule movements. It restricts the CNS movement to 10% of the motion from a glued larva and allows motion of only 10 ± 30m over 350 s immobilization which was sufficient for CNS imaging. These larva-on-a-chip platforms can be useful for studying CNS responses to sensory cues such as sound, light, chemosensory, tactile, and electric/magnetic fields.

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Cardiac screening of intact Drosophila melanogaster larvae under exposure to aqueous and gaseous toxins in a microfluidic device

Abstract: We present a semi-automated microfluidic chip for orientation, immobilization, chemical exposure, and cardiac screening of 3rd instar Drosophila melanogaster larvae.

Cited by 11 publications

References 43 publications

Tools to reverse-engineer multicellular systems: case studies using the fruit fly

Tools to reverse-engineer multicellular systems: case studies using the fruit fly

A microfluidic device to study electrotaxis and dopaminergic system of zebrafish larvae

Characterization of microfluidic clamps for immobilizing and imaging of Drosophila melanogaster larva's central nervous system

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