Cardioprotective effect of Trichopus zeylanicus against myocardial ischemia induced by isoproterenol in rats

Abstract

Cardioprotective effect of Trichopus zeylanicus against myocardial ischemia induced by isoproterenol in rats BJP IntroductionTrichopus zeylanicus Gaerten Trichopodaceae, is a wild plant, a rare genus, small glabrous herb growing in the Agasthyar hilly forests of Kerala, India. The tribal inhabitants (Kani tribe) of this area call this plant ''Arogyappacha'' meaning the greener of health, and use this plant as a health tonic and rejuvenator. This information is based on ethno-medico-botanical investigations (Pushpangadan et al., 1988;Evans et al., 2002). Earlier studies on T. zeylanicus in experimental animals have shown several pharmacological activities such as hepatoproctection, anti-ulcer activity, anti-fatigue, antiinflammatory activity, antioxidant activity, aphrodisiac activity, anti-stress, enhancement of swimming performance and immunomodulation (Sharma et al., 1989;Subramoniam et al., 1997;Singh et al., 2001;Singh et al., 2005). In the present study, an attempt has been made to assess the cardio protective effects of T. zeylanicus leaves on cardiac function in isoproterenol-induced myocardial infarction in rats. Materials and Methods ChemicalsIsoproterenol hydrochloride, thiobarbituric acid, 2,4-dinitrophenyl hydrazine, and glutathione were purchased from Sigma Chemical, Mumbai. All other reagents and chemicals used in this study were of analytical grade with high purity. AnimalsWister strain male albino rats, weighing 100-120 g were selected for the study. The animals were housed individually in polypropylene cages under hygienic and standard environmental conditions (28 ± 2ºC, humidity AbstractThe cardioprotective effect of Trichopus zeylanicus leaves against isoproterenol -induced myocardial ischemia was studied. Wister strain rats were pretreated with T. zeylanicus leaves (500 mg/kg body weight) for 28 days and then intoxicated with isoproterenol (20 mg/100 g, i.p. for 2 consecutive days). Cardioprotection was assessed by estimating plasma and heart aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, and creatine phosphokinase. Troponin T was estimated in serum, and the levels of thiobarbituric acid reactive substances (TBARS) and reduced glutathione (GSH) were analyzed in heart and plasma. In isoproterenol-treated group, shrinkage of cardiac markers in plasma and elevated lipid peroxidation were accompanied by decreased content of reduced glutathione in heart and plasma. The prior administration of T. zeylanicus significantly (p<0.001) prevented the isoproterenol-induced alterations and restored the cardiac markers. These findings indicate the cardioprotective activity of T. zeylanicus during isoproterenol-induced myocardial ischemia.

Cardioprotective effect of Erythrina stricta leaves on isoproterenol-induced myocardial infarction in rat

“…In the present study, α-tocopherol was used as standard as it protects the myocardial membrane against the free radical damage by nature of its anti-oxidant ability (Velavan et al, 2009).…”

Cardioprotective effect of ethanolic extract of Urtica parviflora Roxb. against isoproterenol induced myocardial infarction in rats

“…At the end of the treatment, rats were sacrificed and heart was isolated to measure the organ weight parameters and biochemical parameters such as malondialdehyde, SOD, CAT and reduced GSH. [13]…”

High frequency in vitro propagation of Trichopus zeylanicus subsp. travancoricus using branch–petiole explants

“…The plant is treated as Indian ginseng because of the similar pharmacological properties of ginseng, Panax ginseng (Anilkumar et al 2002). T. zeylanicus possesses several pharmacological activities and properties such as choleretic, aphrodisiac, hepatoprotective, mast cell stabilization (Subramoniam et al 1997(Subramoniam et al , 1998(Subramoniam et al , 1999, adaptogenic (Singh et al 2005), and cardioprotective (Velavan et al 2009). The active fraction of the plant is reported to be effective on phagocytosis by peritoneal macrophages and humoral immune responses in mice (Subramoniam et al 2000).…”