In this study, Antioxidant activity ofCyperus rotundusrhizomes extract (CRRE) was evaluveted in a series ofin vitroassay involving free radicals and reactive oxygen species and IC50values were determined. CRRE exhibited its scavenging effect in concentration dependent manner on superoxide anion radicals, hydroxyl radicals, nitric oxide radical, hydrogen peroxide, and property of metal chelating and reducing power. The extract was also studied for lipid peroxidation assay by thiobarbituric acid–reactive substances (TBARS) using young and aged rat brain mitochondria. The extract was also effective in preventing mitochondrial lipid peroxidation induced by FeSO4/ ascorbate in concentration dependent manner. The results obtained in the present study indicate thatC. rotundusrhizomes extract can be a potential source of natural antioxidant.

Objectives: To Investigate the phytochemicals present in Bryonopsis laciniosa fruit. Methodology: Qualitative, Quantitative screening, Compound Identification by UV-Visible method and identification of functional group of the active chemical components were followed by standard procedures. Result: The results showed the presence of phytonutrients like reducing sugar, terpenoids, triterpenoids, aminoacids, anthroquinone, polyphenols, glycosides, anthocyanins, tannins, coumarins, emodins, saponins, total alkaloids, total flavonoids, lignin and serpentine. These substances may be responsible for the health related properties of the plant which are based on antioxidant,anticancer, antipyretic, antiaphoretic, antidiabetic, anti-inflammation, antiheamatisum, antimicrobial and antiviral activity. Conclusion: This study supports the popular use of Bryonopsis laciniosa fruit in preparation of various pharmaceutical formulations for human welfare.

Introduction:The modulations of glucose-metabolizing enzyme activities play a vital rolein the depletion of energy metabolism and leads to inhibition of cancer growth.Objective:To find the effect of shorearobusta bark extract on glucose-metbolizing enzymes in diethylnitrosamine (DEN) induced hepatocellular carcinoma rats.Materials and Methods:Biochemical evaluation of glucose metabolizing enzyme were done in before and after shorearobusta bark extract (500mg/kg) treatment in DEN induced rats.Results:A significant increasein the activities of the key glycolytic enzymes viz., hexokinase and phosphoglucoisomerase, with a significant decrease in the gluconeogenic enzymes glucose-6-phosphatase and fructose-1,6-bisphosphatasewere observed in HCC bearing rats, when compared with the control. Administration of shorearobusta extract caused a significant decrease in theactivities of glycolytic enzymes and an increase in the gluconeogenic enzymes activities to near normal values.Conclusion:The current findings suggest that the S. robusta extract has a definite modulating role on the key enzymes ofglucose-metabolism in HCC. The modulatory effect may be due to the phytoactive constituents present in the extract of S. robusta.SUMMARY Administration of shorea robusta bark extract caused a significant decrease in the activities of glycolytic enzymes and an increase in the gluconeogenic enzymes activities to near normal values. The S. robusta extract has modulatory activity on the carbohydrate metabolism in DEN-induced HCC bearing rats through a mechanism that which does not provoke any acute biochemical disturbances in the metabolic pathways of glycolysis and gluconeogenesis. The modulatory effect of S. robusta extract may be attributed to the presence of active compounds such as polyphenols and flavonoids. Abbreviations used: HCC: Hepatocellular Carcinoma, SRBE: Shorearobusta bark extract; HEX: Hexokinase; PGI: Phosphoglucoisomerase; DEN: Diethylnitrosamine.

Cardioprotective effect of Trichopus zeylanicus against myocardial ischemia induced by isoproterenol in rats BJP IntroductionTrichopus zeylanicus Gaerten Trichopodaceae, is a wild plant, a rare genus, small glabrous herb growing in the Agasthyar hilly forests of Kerala, India. The tribal inhabitants (Kani tribe) of this area call this plant ''Arogyappacha'' meaning the greener of health, and use this plant as a health tonic and rejuvenator. This information is based on ethno-medico-botanical investigations (Pushpangadan et al., 1988;Evans et al., 2002). Earlier studies on T. zeylanicus in experimental animals have shown several pharmacological activities such as hepatoproctection, anti-ulcer activity, anti-fatigue, antiinflammatory activity, antioxidant activity, aphrodisiac activity, anti-stress, enhancement of swimming performance and immunomodulation (Sharma et al., 1989;Subramoniam et al., 1997;Singh et al., 2001;Singh et al., 2005). In the present study, an attempt has been made to assess the cardio protective effects of T. zeylanicus leaves on cardiac function in isoproterenol-induced myocardial infarction in rats. Materials and Methods ChemicalsIsoproterenol hydrochloride, thiobarbituric acid, 2,4-dinitrophenyl hydrazine, and glutathione were purchased from Sigma Chemical, Mumbai. All other reagents and chemicals used in this study were of analytical grade with high purity. AnimalsWister strain male albino rats, weighing 100-120 g were selected for the study. The animals were housed individually in polypropylene cages under hygienic and standard environmental conditions (28 ± 2ºC, humidity AbstractThe cardioprotective effect of Trichopus zeylanicus leaves against isoproterenol -induced myocardial ischemia was studied. Wister strain rats were pretreated with T. zeylanicus leaves (500 mg/kg body weight) for 28 days and then intoxicated with isoproterenol (20 mg/100 g, i.p. for 2 consecutive days). Cardioprotection was assessed by estimating plasma and heart aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, and creatine phosphokinase. Troponin T was estimated in serum, and the levels of thiobarbituric acid reactive substances (TBARS) and reduced glutathione (GSH) were analyzed in heart and plasma. In isoproterenol-treated group, shrinkage of cardiac markers in plasma and elevated lipid peroxidation were accompanied by decreased content of reduced glutathione in heart and plasma. The prior administration of T. zeylanicus significantly (p<0.001) prevented the isoproterenol-induced alterations and restored the cardiac markers. These findings indicate the cardioprotective activity of T. zeylanicus during isoproterenol-induced myocardial ischemia.

Nanoparticles usually speak of particles with a size up to 100 nm with increasing in surface to volume ratio, providing more active surface atoms to contribute the role in applications and improve the properties of the materials1. The control particle size, particle shape and morphology are very much important in nanoparticles preparation. The most important tool to study the nano materials is X-ray diffraction. X-ray diffraction is one of the most important characterization tools used in solid state chemistry and materials science. XRD is an easy tool to determine the size and the shape of the unit cell for any compound. In this study explaining the formation of silver nanoparticles through Erythrina indica flowers. The synthesized AgNPs further examined the X-ray Diffraction (XRD) and Energy Dispersive Spectroscopy (EDS) analysis. The X-ray diffraction results clearly show that the silver nanoparticles formed by the reduction of Ag+ ions by the Erythrina indica flower extract are crystalline in nature. The average crystalline size of AgNPs is calculated using Debye-Scherrer formula is ~28.19 nm. The EDX reading proved that the required phase of silver (Ag) is present in the sample.