Nanoparticles usually speak of particles with a size up to 100 nm with increasing in surface to volume ratio, providing more active surface atoms to contribute the role in applications and improve the properties of the materials1. The control particle size, particle shape and morphology are very much important in nanoparticles preparation. The most important tool to study the nano materials is X-ray diffraction. X-ray diffraction is one of the most important characterization tools used in solid state chemistry and materials science. XRD is an easy tool to determine the size and the shape of the unit cell for any compound. In this study explaining the formation of silver nanoparticles through Erythrina indica flowers. The synthesized AgNPs further examined the X-ray Diffraction (XRD) and Energy Dispersive Spectroscopy (EDS) analysis. The X-ray diffraction results clearly show that the silver nanoparticles formed by the reduction of Ag+ ions by the Erythrina indica flower extract are crystalline in nature. The average crystalline size of AgNPs is calculated using Debye-Scherrer formula is ~28.19 nm. The EDX reading proved that the required phase of silver (Ag) is present in the sample.