In this study molecular imprinting technology was employed to prepare a specific affinity sorbent for the resolution of Cathine, a chiral drug product. The molecularly imprinted polymer (MIP) was prepared by non-covalent molecular imprinting with either (+) or (-)-Cathine (threo-2-amino-1-hydroxy-1-phenyl propane; norpseudoephedrine) as the template. Methacrylic acid and ethylene glycol di-methacrylate were copolymerized in the presence of the template molecule. The bulk polymerization was carried out in chloroform with 2,2'-azobisisobutyronitrile as the initiator, at 5 °C and under UV radiation. The resulting MIP was ground into powders, which were slurry packed into analytical columns. After removal of template molecules, the MIP-packed columns were found to be effective for the resolution of (±)-Cathine racemates. The separation factor for the enantiomers ranged between 1.5 and 2.4 when the column was packed with MIP prepared with (+)-Cathine as the template. A separation factor ranging from 1.6 to 2.9 could be achieved from the column packed with MIP, prepared with (-)-Cathine as the template. Although the separation factor was higher with that previously obtained from reversed-phase column chromatography following derivatization with a chiral agent, elution peaks were broader due to the heterogeneity of binding sites on MIP particles and the possible non-specific interaction.

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Introduction:The modulations of glucose-metabolizing enzyme activities play a vital rolein the depletion of energy metabolism and leads to inhibition of cancer growth.Objective:To find the effect of shorearobusta bark extract on glucose-metbolizing enzymes in diethylnitrosamine (DEN) induced hepatocellular carcinoma rats.Materials and Methods:Biochemical evaluation of glucose metabolizing enzyme were done in before and after shorearobusta bark extract (500mg/kg) treatment in DEN induced rats.Results:A significant increasein the activities of the key glycolytic enzymes viz., hexokinase and phosphoglucoisomerase, with a significant decrease in the gluconeogenic enzymes glucose-6-phosphatase and fructose-1,6-bisphosphatasewere observed in HCC bearing rats, when compared with the control. Administration of shorearobusta extract caused a significant decrease in theactivities of glycolytic enzymes and an increase in the gluconeogenic enzymes activities to near normal values.Conclusion:The current findings suggest that the S. robusta extract has a definite modulating role on the key enzymes ofglucose-metabolism in HCC. The modulatory effect may be due to the phytoactive constituents present in the extract of S. robusta.SUMMARY Administration of shorea robusta bark extract caused a significant decrease in the activities of glycolytic enzymes and an increase in the gluconeogenic enzymes activities to near normal values. The S. robusta extract has modulatory activity on the carbohydrate metabolism in DEN-induced HCC bearing rats through a mechanism that which does not provoke any acute biochemical disturbances in the metabolic pathways of glycolysis and gluconeogenesis. The modulatory effect of S. robusta extract may be attributed to the presence of active compounds such as polyphenols and flavonoids. Abbreviations used: HCC: Hepatocellular Carcinoma, SRBE: Shorearobusta bark extract; HEX: Hexokinase; PGI: Phosphoglucoisomerase; DEN: Diethylnitrosamine.

Secondary operation of boring is carried out to produce close geometric tolerance holes produced in drilling. In this work, an attempt has been made in producing a hole by dry boring, wet boring and cryogenic boring of gunmetal. Gunmetal finds its application in areas of casting, steam turbines and pressure valves, due to its high resistance to corrosion. Investigation of cutting temperature generated during machining, cutting force experienced by the tool and surface roughness of the machined workpiece was toted out with analogous to cutting speed and feed, by Taguchi, ANOVA and TOPSIS analyses. Surface morphology study, tool wear analysis, residual stress analysis were done in order to distinguish the modification in the bored surface and tool material after machining. Cryogenic boring of gunmetal reduced the cutting temperature by 63.43% and 53.85% over dry boring and wet boring, respectively. Furthermore, cutting force is reduced by 34.51% and 18.29% over dry boring and wet boring, respectively. Additionally, a decrease of 62.23% and 35.10% is observed in surface roughness on comparing dry boring and wet boring with respect to cryogenic boring of gunmetal. Taguchi and ANOVA arbitrated speed as an imperative parameter for cutting force and feed as a paramount parameter in cutting temperature. Furthermore, speed is a crucial parameter for arbitrating surface roughness in dry boring and wet boring, additionally feed for surface roughness in cryogenic boring condition. TOPSIS analysis illuminated speed of 1200 rpm and feed of 0.055 mm/min as the most closest to exemplar elucidation for all three cutting preconditions. Aggrandizement in compressive residual stress is discerned in cryogenic boring by 39.74% and 19.35% correlated with dry boring and wet boring, respectively, as the material undergoes work hardening. A decrease in residual tensile stress is espied in cryogenic boring when juxtaposed to wet boring, ensuring upsurge in tool life and better surface characteristics. Microhardness in cryogenic boring of gunmetal improves by 5.83-11.05% and 0.51-2.57% analogous to dry boring and wet boring, respectively. Corrosion resistance in cryogenic boring increases by 77.43% and 49.25% analogous to dry boring and wet boring of gunmetal, respectively. The circularity and cylindricity increase by 4.76-98.40%, 8.33-97.50% and 6.25-97.15%, 2.43-92.85% in cryogenic boring juxtaposed to dry boring and wet boring of gunmetal, respectively.

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