Cardioprotective effects of fucoidan against hypoxia-induced apoptosis in H9c2 cardiomyoblast cells

Abstract

Context: Cardiomyocyte apoptosis plays a critical role in the progress of heart diseases. Fucoidan, a complex-sulfated polysaccharide, has been reported to possess potential cardioprotective efficacy in vivo. Objective: The present study determines whether fucoidan could provide cardioprotection on hypoxia-induced cardiomyocyte apoptosis. Materials and methods: H9c2 cardiomyoblast cells were incubated with various concentrations (15, 30, and 60 lg/ml) of fucoidan in a humidified incubator at 37 C with 95% O 2 and 5% CO 2 . After 6 h, hypoxia was processed and the cardioprotective effects of fucoidan were evaluated by applying MTT, ELISA, Hoechst 33258 nucleus staining, and western blot.Results: Following a 6 h exposure of H9c2 to hypoxic condition, significant reduction was found in cell survival (0.57-fold) and superoxide dismutase (SOD) activity (0.56-fold), which were associated with the increase of malondialdehyde (MDA) level (2.58-fold), creatine phosphokinase (CK, 3.57-fold), and lactate dehydrogenase (LDH) activities (2.39-fold). Moreover, hypoxiainduced apoptosis was confirmed by Hoechst 33258 nuclear staining, and these changes were accompanied by the increase of Bcl-2 (1.27-fold) and Bax expression (2.6-fold). However, preincubation of the cells with fucoidan prior to hypoxia exposure elevated the cell viability (30 lg/ml, 1.18-fold; 60 lg/ml, 1.32-fold) and SOD activity (30 lg/ml, 1.12-fold; 60 lg/ml, 1.25-fold), but decreased the MDA level (30 lg/ml, 0.70-fold; 60 lg/ml, 0.80-fold), CK (30 lg/ml, 0.69-fold; 60 lg/ml, 0.76-fold), and LDH (30 lg/ml, 0.67-fold; 60 lg/ml, 0.86-fold) leakages. Hoechst 33258 nuclear staining observations demonstrated the same protective effect of fucoidan on hypoxia-induced myocardial injury. Also, cardioprotective effects of fucoidan were reflected by increasing Bcl-2 (60 lg/ml, 1.84-fold), as well as decreasing Bax (60 lg/ml, 0.6-fold). Conclusion: Fucoidan had protective effect against hypoxia-induced cardiomyocytes apoptosis, and the mechanism might involve protections of the cell from oxidative injury.

Quality by design‐based optimization of formulation and process parameters for berberine nanosuspension for enhancing its dissolution rate, bioavailability, and cardioprotective activity

“…The relative mean fold change of the target gene present in test sample with respect to the average control was calculated. The results were presented in terms of 2 −ΔΔCt value that resembles 2 to the power of negative ΔΔCt, as reported earlier (Zhang et al, 2015).…”

“…The experiment was repeated thrice and the mean cell viability was calculated. LDH release assay was also performed using LDH release kit (Thermofisher) as per specified protocol (Zhang et al, 2015).…”

Fucoidan attenuates radioiodine-induced salivary gland dysfunction in mice

“…Moreover, numerous studies have found that fucoidan is protective against tissue damage. Zhang et al [23] reported that fucoidan was protective against hypoxia-induced cardiomyocyte apoptosis, and the mechanism might involve protecting the cells from oxidative injury. Rhee et al [24] also reported that fucoidan had protective effects on the radiation-induced damage of blood cells.…”

“…[27] reported fucoidan changes the expression patterns of inflammatory cytokines and it attenuate tissue inflammation and fibrosis. Fucoidan-dependent effects are thought to be caused by its scavenging action on ROS and a modulation of inflammation mediator and apoptosis as a radiation mitigator [23, 24, 27].…”

Prospects for the therapeutic application of sulfated polysaccharides of brown algae in diseases of the cardiovascular system: review

“…The antioxidant properties of fucoidans from Canistrocarpus cervicornis (Camara et al 2011), Lessonia vadosa (Barahona et al 2011), A. nodosum (Yuan & Macquarrie 2015), Dictyota dichotoma, Drosera indica, Iyengaria stellata, Padina pavonia, Sargassum swartzii, Sargassum variegatum, Stoechospermum marginatum, Stokeyia indica, Jolyna laminarioides, Caulerpa taxifolia, Halimeda tuna, Ulva fasciata, Ulva lactuca, Solieria robusta and Melanothamnus afaqhusainii (Tariq et al 2015) demonstrate potential use for the inhibition of free-radical processes in atherosclerosis. Zhang et al (2015a) showed that preincubation of the H9c2 cells with fucoidan prior to hypoxia exposure elevated cell viability and decreased the concentration of malondialdehyde and lactate dehydrogenase.…”