Cardioprotective mechanisms ofPrunus cerasus(sour cherry) seed extract against ischemia-reperfusion-induced damage in isolated rat hearts

Abstract

Cardioprotective mechanisms of Prunus cerasus (sour cherry) seed extract against ischemia-reperfusion-induced damage in isolated rat hearts. Am J Physiol Heart Circ Physiol 291: H1329 -H1336, 2006. First published April 14, 2006 doi:10.1152/ajpheart.01243.2005The effects of kernel extract obtained from sour cherry (Prunus cerasus) seed on the postischemic cardiac recovery were studied in isolated working rat hearts. Rats were treated with various daily doses of the extract for 14 days, and hearts were then isolated and subjected to 30 min of global ischemia followed by 120 min of reperfusion. The incidence of ventricular fibrillation (VF) and tachycardia (VT) fell from their control values of 92% and 100% to 50% (not significant) and 58% (not significant), 17% (P Ͻ 0.05), and 25% (P Ͻ 0.05) with the doses of 10 mg/kg and 30 mg/kg of the extract, respectively. Lower concentrations of the extract (1 and 5 mg/kg) failed to significantly reduce the incidence of VF and VT during reperfusion. Sour cherry seed kernel extract (10 and 30 mg/kg) significantly improved the postischemic recovery of cardiac function (coronary flow, aortic flow, and left ventricular developed pressure) during reperfusion. We have also demonstrated that the extract-induced protection in cardiac function significantly reflected in a reduction of infarct size. Immunohistochemistry indicates that a reduction in caspase-3 activity and apoptotic cells by the extract, beside other potential action mechanisms of proanthocyanidin, trans-resveratrol, and flavonoid components of the extract, could be responsible for the cardioprotection in ischemic-reperfused myocardium.

Cardioprotection Afforded by Sour Cherry Seed Kernel

“…Previous work by the authors has demonstrated potent cardioprotective properties of HO-1 (10,11). These outcomes are consistent with many investigations demonstrating that phytochemical HO-1 induction correlates with improved prognoses of many diseases (12).We have previously shown that SCSE strongly protects the heart against I/R injury, as revealed by enhanced postischemic left ventricle function, reduced infarct size, and decreased apoptosis in rat hearts subjected to I/R-injury (13). Recent animal studies also demonstrated SCSE-mediated enhancement of SCSE-mediated adaptive response in hearts of hypercholesterolemic rabbits (14).…”

“…Briefly, following removal of external shell components, the dried seed kernels were ground and extracted with n-hexane, followed by vacuum evaporation and defatting to yield a standardized extract suitable for use as a feed supplement (13).…”

“…6 performed under terminal anesthesia, followed by excision of hearts and placement of the organs in ice-cold modified Krebs-Henseleit buffer, as previously described (13). After the thoracotomy, the hearts were cannulated through the aorta and perfused in a Langendorff apparatus in "non-working" mode (100 cm H 2 O) for 5 minutes to cleanly flush blood from vessels of the organ.…”

Dietary factors and lung function in the general population: wine and resveratrol intake

“…grape juice and fortified wine or other sources suggested to contain resveratrol, such as hops, which are present in beer [35], or cherries [36]. Nevertheless, our estimation of resveratrol intake might be acknowledged as sensitive on a population level, as the sources that were not used contain a low concentration of resveratrol [27,35,36] and/or are not widely consumed in the total population.…”

Hepatoprotective Effect of Microemulsion-Based System of Prunus Cerasus Kernel Extract on CCL4-induced Liver Damage in Mice

“…The extract was filtered through a clean cotton cloth, and then, solvent was evaporated using a rotary evaporator at 40°C temperature in vacuum. The extract doses were selected on the basis of experiments conducted in the university laboratory and earlier reports (17). Therefore, with regard to the effective doses of Prunus cerasus kernel extract in mice (250, 500, and 1000 mg/kg), the current study used the same doses considering the body weight of the mice rather than the base ME; thus, 2.5%, 5%, and 10% ME extracts were obtained.…”