Hepatoprotective Effect of Microemulsion-Based System of Prunus Cerasus Kernel Extract on CCL4-induced Liver Damage in Mice

Kalantari‬, Heibatullah; Salimi, Anayatollah; Motaharitabar, Eisa; Samimi, Azin; Rezai, Annahita

doi:10.5812/jjnpp.14282

Cited by 2 publications

(2 citation statements)

References 33 publications

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“…Saco) on nitric oxide level was previously reported in HepG2 cells incubated with sodium nitroprusside due to inducible nitric oxide synthase (iNOS) ligand modulation by phenolic compounds, which was also demonstrated by the docking results [22]. Protection against hepatic injury induced in mice was revealed in the case of P. cerasus kernel [66] and black cherry (P. serotina Ehrh.) [21] extract administration.…”

Section: Effect On Ros Lipid Peroxidation and Nitric Oxidementioning

confidence: 66%

The Chemical Profile, Antioxidant, and Anti-Lipid Droplet Activity of Fluid Extracts from Romanian Cultivars of Haskap Berries, Bitter Cherries, and Red Grape Pomace for the Management of Liver Steatosis

Craciunescu,

Seciu-Grama,

Mihai

et al. 2023

IJMS

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This study aimed to investigate, for the first time, the chemical composition and antioxidant activity of fluid extracts obtained from three Romanian cultivars of haskap berries (Lonicera caerulea L.) var. Loni, bitter cherries (Prunus avium var. sylvestris Ser.) var. Silva, and pomace from red grapes (Vitis vinifera L.) var. Mamaia, and their capacity to modulate in vitro steatosis, in view of developing novel anti-obesity products. Total phenolic, flavonoid, anthocyanin, and ascorbic acid content of fluid extracts was spectrophotometrically assessed and their free radical scavenging capacity was evaluated using Trolox Equivalent Antioxidant Capacity (TEAC) and free 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical inhibition assays. The Pearson coefficients showed a moderate correlation between the antioxidant activity of fluid extracts and their phenolic content, but a strong correlation between anthocyanin and ascorbic acid content. HPLC analysis identified and quantified the main phenolic compounds of chlorogenic and syringic acid, catechin, and glycosylated kaempferol, apigenin, and quercetin, in variable proportions. An in vitro experimental model of steatosis was developed in HepG2 hepatocytes treated with a mixture of free fatty acids. Cell culture analyses showed that cytocompatible concentrations of fluid extracts could significantly reduce the lipid accumulation and inhibit the reactive oxygen species, malondialdehyde, and nitric oxide secretion in stressed hepatocytes. In conclusion, these results put an emphasis on the chemical compounds’ high antioxidant and liver protection capacity of unstudied fluid extracts obtained from Romanian cultivars of bitter cherries var. Silva and pomace of red grapes var. Mamaia, similar to the fluid extract of haskap berries var. Loni, in particular, the positive modulation of fat deposition next to oxidative stress and the lipid peroxidation process triggered by fatty acids in HepG2 hepatocytes. Consequently, this study indicated that these fluid extracts could be further exploited as hepatoprotective agents in liver steatosis, which provides a basis for the further development of novel extract mixtures with synergistic activity as anti-obesity products.

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Section: Effect On Ros Lipid Peroxidation and Nitric Oxidementioning

confidence: 66%

The Chemical Profile, Antioxidant, and Anti-Lipid Droplet Activity of Fluid Extracts from Romanian Cultivars of Haskap Berries, Bitter Cherries, and Red Grape Pomace for the Management of Liver Steatosis

Craciunescu,

Seciu-Grama,

Mihai

et al. 2023

IJMS

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“…The final formulation was evaluated for phase separation, cracking, and coalescence [31] . The prepared microemulsion batches were centrifuged at 1,000 rpm for 1 hour and at 3,000 rpm for 1 hour, and afterwards the phase separation was evaluated [32] . A dynamic lightscattering technique was used for the determination of particle size using the instrument Zetasizer (Malvern Zetasizer ver.…”

Section: Characterizationmentioning

confidence: 99%

Development of bioflavonoid containing chemotherapeutic delivery systems for UV-damaged skin and kangri cancer

Sharma¹,

Sahu

Kaur³

2021

Forum of Clinical Oncology

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Background The lower abdomen and inner thighs are most likely to become affected by kangri cancer because those areas are exposed to continuous exposure to kangri. Objective In this article, formulation and characterization of a water-in-oil microemulsion of 5-fluorouracil with rutin (R-5FU) for better skin penetration and inhibition of kangri cancer (skin cancer surfactant) is discussed. Method To produce R-5-FU microemulsions, surfactant-cosurfactant was mixed with oil. Distilled water was added dropwise with the help of a burette by gentle stirring at a constant temperature. The surfactant and co-surfactant were mixed into three particular ratios 1:1, 2:1, and 3:1. Further characterizations were performed, such as visual inspection and thermodynamic stability including a stress test and centrifugation. In visual inspection included assessment of the colour, homogeneity, and odour of the formulation of FU microemulsion. Result All three microemulsions, labeled RME1, RME2, and RME3, are highly stable. An oval shape of surface morphology of 5-FU was noticed by using a TEM image. The viscosity of RME3 was found to be 17.25±0.22 pa-s. The average globule size was 100–300 nm for all three RME. The results of human cadaver skin permeability are almost of the same pattern, butRME3 indicates the best skin permeability with negligible side effects on the skin. Conclusion The quantity of 5-FU released from all formulations at 3-hr ranged from 95.57% to 83.67%. None of the three formulations resulted in skin irritation, with irritancy score of zero (IS=0). Observation revealed no lysis, hemorrhage, or coagulation after application.

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Hepatoprotective Effect of Microemulsion-Based System of Prunus Cerasus Kernel Extract on CCL4-induced Liver Damage in Mice

Abstract: Background: Liver diseases are among the public health concerns that significantly contribute to the global burden of mortality and illness. Herbal medicines are always used to treat a great number of diseases such as hepatotoxicity worldwide.

Cited by 2 publications

References 33 publications

The Chemical Profile, Antioxidant, and Anti-Lipid Droplet Activity of Fluid Extracts from Romanian Cultivars of Haskap Berries, Bitter Cherries, and Red Grape Pomace for the Management of Liver Steatosis

The Chemical Profile, Antioxidant, and Anti-Lipid Droplet Activity of Fluid Extracts from Romanian Cultivars of Haskap Berries, Bitter Cherries, and Red Grape Pomace for the Management of Liver Steatosis

Development of bioflavonoid containing chemotherapeutic delivery systems for UV-damaged skin and kangri cancer

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