Background: Considering the importance of pre-hospital resuscitation, it is necessary to update staff on the relevant knowledge and skills through education, sufficient practice, good supervision and recruitment of appropriately qualified cadres. Aims: This study aimed to determine the association between knowledge and skills for emergency medical services (EMS) of basic life support in northwest Khuzestan province, Islamic Republic of Iran, in 2016. Methods: This cross-sectional study was conducted on all EMS (75 participants) in two emergency centers in the southwest of the Islamic Republic of Iran. The sampling was done via the available census. Three questionnaires were used: 1) demographic information (age, time of participation in retraining classes, academic degree, and employment); 2) knowledge of basic life support (BLS); and 3) checklist of BLS and automated external defibrillator (AED) skills. Data analysis was done using descriptive statistics, chi-square, ANOVA and Kruskal-Wallis test. Results: 30.7% of the emergency medical services had poor knowledge (mean score was 19.35 1 / 10 WHO EMRO | Relationship between knowledge and skill for basic life support in emergency medical serv ± 3.9; range: 0-34) and 42.7% of subjects had poor skills (mean score was 5.40 ± 2.39; range: 0-9). Both results are higher than the national average. The findings also indicated that 70.7% of staff did not use AED devices correctly, and there was a significant relationship between staff knowledge and skills with AED device use (P < 0.05). Among the 41 participants that had taken part in training classes 12 months previously, more than 50% had poor skills (n=21) and only 7 participants had good skills. Also, among the 11 participants who had taken part in training classes 3 months ago, 7 participants had good skills. In general, there was no relationship between staff knowledge and skill (P > 0.05). Conclusion: The results showed that none of the participants acquired a full knowledge score and only 6 participants gained the total skill score.