Cardiovascular effects of Sp-CTx, a cytolysin from the scorpionfish (Scorpaena plumieri) venom

Gomes, Helena L.; Menezes, Thiago N.; Malacarne, Pedro Felipe; Roman‐Campos, Danilo; Gondim, Antônio Nei Santana; Cruz, Jáder Santos; Vassallo, Dalton Valentim; Figueiredo, Suely G.

doi:10.1016/j.toxicon.2016.05.002

Cited by 12 publications

(23 citation statements)

References 43 publications

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“…In isolated papillary muscle, Sp-CTx produces a positive inotropic effect attenuated by propranolol and the catecholamine releasing agent tyramine, while increasing L-type Ca 2+ current density in isolated ventricular cardiomyocytes. These results show that Sp-CTx induces cardiovascular disorders through an increase of sarcolemmal calcium influx, partially due to the release of endogenous noradrenaline [26]. In addition, Sp-CTx reproduced the SpV-induced effect on aortic rings, although the relaxation phase is less marked in this case.…”

Section: Bioactive Proteins Isolated From Spvmentioning

confidence: 76%

A review on the Scorpaena plumieri fish venom and its bioactive compounds

Campos

Menezes

Malacarne

et al. 2016

J Venom Anim Toxins Incl Trop Dis

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The most poisonous fish species found along the Brazilian coast is the spotted scorpionfish Scorpaena plumieri. Though hardly ever life-threatening to humans, envenomation by S. plumieri can be quite hazardous, provoking extreme pain and imposing significant socioeconomic costs, as the victims may require days to weeks to recover from their injuries. In this review we will walk the reader through the biological features that distinguish this species as well as the current epidemiological knowledge related to the envenomation and its consequences. But above all, we will discuss the challenges involved in the biochemical characterization of the S. plumieri venom and its compounds, focusing then on the successful isolation and pharmacological analysis of some of the bioactive molecules responsible for the effects observed upon envenomation as well as on experimental models. Despite the achievement of considerable progress, much remains to be done, particularly in relation to the non-proteinaceous components of the venom. Therefore, further studies are necessary in order to provide a more complete picture of the venom’s chemical composition and physiological effects. Given that fish venoms remain considerably less studied when compared to terrestrial venoms, the exploration of their full potential opens a myriad of possibilities for the development of new drug leads and tools for elucidating the complex physiological processes.

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Section: Bioactive Proteins Isolated From Spvmentioning

confidence: 76%

A review on the Scorpaena plumieri fish venom and its bioactive compounds

Campos

Menezes

Malacarne

et al. 2016

J Venom Anim Toxins Incl Trop Dis

Self Cite

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“…In addition, CfTX have the homology structure that is with three-domain Cry toxins (δ-endotoxins) and αhelices in N-terminal domain [33,34] . This type of pore-forming toxin was reported accelerated the rst temporal derivative of the isosmetric contraction (dP/dt) [32] . Its speci c lipid-dependent cell penetration induces a transient membrane leakage for cardiomyocyte interaction at the atomic level, involved in isometric force [35] , contributed to intracellular Ca 2+ release, improving cAMP and PKA activity in cardiomyocytes [36] .…”

Section: Discussionmentioning

confidence: 96%

The static preload associated myogenic spontaneous fasciculation response in lengthening cardiac muscle

Fan¹,

Gao²,

Ac³

et al. 2021

Preprint

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The passive tension force enhancement is one kind of myogenic spontaneous fasciculation in muscles. However, its physiological properties in cardiac fibres are not well known. In this study, mice cardiac papillary muscle spontaneous force enhancement was evaluated by micro stepping stretch method. The occurrence of spontaneous force and real time cardiac fibre Ca2+ redistribution was tranced by Flou-3 (2mM) indicator. Force enhancement amplitude, enhancement prolonging time, and tension–time integral were analysis by myograph analyser. The results indicated that the spontaneous force occurred immediately after the active stretch, rapidly enhanced during tolerating the sustained static stretch. The force occurrence and amplitude enhance synchronized with the Ca2+ recruitment and lightning transmitted to adjacent fibres. In high preload fibres, the enhancement was forceful to over its maximum passive tension (6.20 ± 0.51 N/mm2 to 4.49 ± 0.43 N/mm2). The force occurrences were unsteadiness in each stretch. The increased enhancement amplitude combining with the shortening prolonging time induced reduction of tension–time integral. We concluded that the intracellular Ca2+ synchronized force enhancement is one kind of interruption event in overloading cardiac fibres. This interruption occurred during the relaxation processing in cardiac muscle, therefore affect the rhythmic stability of cardiac relaxation-contraction cycle.

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“…CfTX-1 act as the pore-forming toxin, have ion channel modulating characteristics. CfTX-1 skinned cardiac muscle membrane disrupting normal transmembrane ion concentration gradients in cardiomyocyte , accelerate the first temporal derivative of the isometric contraction (dP/dt) 42 . Its specific lipid-dependent cell penetration induces transient membrane leakage for cardiomyocyte interactions at the atomic level, is involved in isometric force 43 , contributes to intracellular Ca 2+ release, and improves cAMP and PKA activity in cardiomyocytes 44 .…”

Section: Discussionmentioning

confidence: 99%

Spontaneous myogenic fasciculation associated with the lengthening of cardiac muscle in response to static preloading

Fan

Gao

Bell

et al. 2021

Sci Rep

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Force enhancement is one kind of myogenic spontaneous fasciculation in lengthening preload striated muscles. In cardiac muscle, the role of this biomechanical event is not well established. The physiological passive property is an essential part for maintaining normal diastole in the heart. In excessive preload heart, force enhancement relative erratic passive properties may cause muscle decompensating, implicate in the development of diastolic dysfunction. In this study, the force enhancement occurrence in mouse cardiac papillary muscle was evaluated by a microstepping stretch method. The intracellular Ca2+ redistribution during occurrence of force enhancement was monitored in real-time by a Flou-3 (2 mM) indicator. The force enhancement amplitude, the enhancement of the prolongation time, and the tension–time integral were analyzed by myography. The results indicated that the force enhancement occurred immediately after active stretching and was rapidly enhanced during sustained static stretch. The presence of the force and the increase in the amplitude synchronized with the acquisition and immediate transfer of Ca2+ to adjacent fibres. In highly preloaded fibres, the enhancement exceeded the maximum passive tension (from 4.49 ± 0.43 N/mm2 to 6.20 ± 0.51 N/mm2). The occurrence of force enhancement were unstable in each static stretch. The increased enhancement amplitude combined with the reduced prolongation time to induce a reduction in the tension–time integral. We concluded that intracellular Ca2+-synchronized force enhancement is one kind of interruption event in excessive preload cardiac muscle. During the cardiac muscle in its passive relaxation period, the occurrence of this interruption affected the rhythmic stability of the cardiac relaxation cycle.

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Cardiovascular effects of Sp-CTx, a cytolysin from the scorpionfish (Scorpaena plumieri) venom

Cited by 12 publications

References 43 publications

A review on the Scorpaena plumieri fish venom and its bioactive compounds

A review on the Scorpaena plumieri fish venom and its bioactive compounds

The static preload associated myogenic spontaneous fasciculation response in lengthening cardiac muscle

Spontaneous myogenic fasciculation associated with the lengthening of cardiac muscle in response to static preloading

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