Cariogenicity of Common Sugars as evaluated in the Artificial Mouth

Pigman, Ward; Brasher, James; Koulourides, T.

doi:10.1038/195190b0

Cited by 7 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This experimental setup focused on identifying conditions that favour cariogenesis; Pigman's model is arguably an ancestor to contemporary drip‐fed systems (discussed later in this review). From the 1950s to the 1960s, many in vitro oral studies improved Pigman's artificial mouth system by including an incubator cabinet and sterilization with ethylene oxide (Pigman et al, 1955, 1962; Pigman & Newbrun, 1962). From a fundamental perspective, these studies linked common dietary sugars, for example, glucose and sucrose, to cariogenicity.…”

Section: Past and Present: Oral In Vitro Biofilm Modelsmentioning

confidence: 99%

In vitro model systems for exploring oral biofilms: From single‐species populations to complex multi‐species communities

Luo

Vanek

González-Cabezas

et al. 2021

J of Applied Microbiology

View full text Add to dashboard Cite

Numerous in vitro biofilm model systems are available to study oral biofilms. Over the past several decades, increased understanding of oral biology and advances in technology have facilitated more accurate simulation of intraoral conditions and have allowed for the increased generalizability of in vitro oral biofilm studies. The integration of contemporary systems with confocal microscopy and 16S rRNA community profiling have enhanced the capabilities of in vitro biofilm model systems to quantify biofilm architecture and analyze microbial community composition. In this review, we describe several model systems relevant to modern in vitro oral biofilm studies: the constant depth film fermenter, Sorbarod perfusion system, drip-flow reactor, modified Robbins device, flowcells, and microfluidic systems. We highlight how combining these systems with confocal microscopy and community composition analysis tools aids exploration of oral biofilm development under different conditions and in response to antimicrobial/anti-biofilm agents. The review closes with a discussion of future directions for the field of in vitro oral biofilm imaging and analysis. Introduction: Importance of in vitro model systems to the study of oral biofilmsMicroorganisms form dynamic multi-species biofilm communities on numerous surfaces in the human oral cavity (Marsh 2009). Over time, oral biofilms change in composition and architecture as component microbes interact with each other, the environment, and the host (Lamont et al. 2018). Oral biofilm communities can be extremely resilient; redeveloping rapidly after physical perturbations (e.g. brushing or flossing) and chemical treatments (e.g. application of mouthwash) (Marsh 2010). Furthermore, certain ecological and environmental conditions can alter the microbial composition and behavior of oral biofilm communities resulting in dental caries and periodontal disease (Aas et al. 2008;Peterson et al. 2013;Marsh 2018). Dental caries and periodontal disease are among the most prevalent of human diseases (Petersen et al. 2005) ranking 1 and 11 in a 2016 ranking of global health burden of 328 diseases (Vos and Collaborators 2017). In 2016, an estimated 2.44 billion people had active dental caries while

show abstract

Section: Past and Present: Oral In Vitro Biofilm Modelsmentioning

confidence: 99%

In vitro model systems for exploring oral biofilms: From single‐species populations to complex multi‐species communities

Luo

Vanek

González-Cabezas

et al. 2021

J of Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…Following points have been reported in basic researches regarding with mechanism of caries prevention by fluoride ion [15][16][17][18][19][20][21] ;…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

Transition of Fluoride into Tooth Substance from Sustained Fluoride-Releasing Sealant - In vitro Evaluation -

Motohashi

Furukawa

Shimoda

et al. 2010

J. Hard Tissue Biology.

View full text Add to dashboard Cite

Abstract:How transition of fluorine from orally applied sealant into dental matrix occurs and inhibits caries remains unknown. In the current study, we investigated mechanism of caries protection evaluating movement of fluoride ions from sustained-releasing fluoride sealant by EPMA from a crystallographic point and also from inorganic chemical view. Furthermore, we also evaluated differences of 2 kinds of sealant as a fluoride source, which have different releasing mechanism.Our results demonstrated that fluoride ion released from sealant abundantly accumulated in porous structure of tooth. From this result, it was considered that fluoride ion contributed to caries inhibition by infiltrating into porous structure appeared due to decalcification and enhancing pH environment. This result suggests thinking morphological viewpoint upon efficiency of fluorine in clinical dentistry for caries. When 2 different sealant materials were compared, GI-based sealant significantly released more fluoride ion than resin-based fluorine. Moreover GI-based sealant fluoride ion infiltrated significantly much more into decalcified area than resinbased fluorine. Thus in clinical dentistry, selection of GI-based fluoride ion at early periods and then alternation to long-term keeping resin-based fluorine should be considered to treat caries in a better way.

show abstract

Effects of calcium, phosphate and fluoride ions on the rate of softening and dissolution of tooth enamel

Koulourides

Reed

1964

Archives of Oral Biology

View full text Add to dashboard Cite

Cariogenicity of Common Sugars as evaluated in the Artificial Mouth

Cited by 7 publications

References 7 publications

In vitro model systems for exploring oral biofilms: From single‐species populations to complex multi‐species communities

In vitro model systems for exploring oral biofilms: From single‐species populations to complex multi‐species communities

Transition of Fluoride into Tooth Substance from Sustained Fluoride-Releasing Sealant - In vitro Evaluation -

Effects of calcium, phosphate and fluoride ions on the rate of softening and dissolution of tooth enamel

Contact Info

Product

Resources

About