Carolina rinse solution minimizes kidney injury and improves graft function and survival after prolonged cold ischemia1

Yin, Meizhen; Currin, Robert T.; Peng, Xing-Xi; Mekeel, Harold E.; Schoonhoven, Robert; Lemasters, John J.

doi:10.1097/00007890-200205150-00009

Cited by 12 publications

(10 citation statements)

References 36 publications

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“…Indeed, little difference was observed between the nitrogen and air groups. During cold-storage numerous processes, in addition to ischemia, may affect renal function after transplantation as a result of hypothermia, including cell swelling, microtubule disruption, and protein relocalization [32]. This work reports empirical results and the authors are aware of the fact that no mechanisms of action have been investigated yet.…”

Section: Discussionmentioning

confidence: 99%

“…Organ damage will occur during cold ischemia preservation and will worsen after reperfusion. The ex vivo preserved organ will display an anaerobic metabolism and produce a massive amount of reactive oxygen species (ROS) upon reperfusion, which are correlated with ischemia-reperfusion injury [6,7,8]. Cold-storage techniques were developed in order to reduce ROS induction and these techniques have contributed to major improvement in renal transplantation over the last few decades.…”

Section: Introductionmentioning

confidence: 99%

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Noble Gas (Argon and Xenon)-Saturated Cold Storage Solutions Reduce Ischemia-Reperfusion Injury in a Rat Model of Renal Transplantation

et al. 2012

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Background: Following kidney transplantation, ischemia-reperfusion injury contributes to adverse outcomes. The purpose of this study was to determine whether a cold-storage solution saturated with noble gas (xenon or argon) could limit ischemia-reperfusion injury following cold ischemia. Methods: Sixty Wistar rats were randomly allocated to 4 experimental groups. Kidneys were harvested and then stored for 6 h before transplantation in cold-storage solution (Celsior®) saturated with either air, nitrogen, xenon or argon. A syngenic orthotopic transplantation was performed. Renal function was determined on days 7 and 14 after transplantation. Transplanted kidneys were removed on day 14 for histological and immunohistochemical analyses. Results: Creatinine clearance was significantly higher and urinary albumin significantly lower in the argon and xenon groups than in the other groups at days 7 and 14. These effects were considerably more pronounced for argon than for xenon. In addition, kidneys stored with argon, and to a lesser extent those stored with xenon, displayed preserved renal architecture as well as higher CD-10 and little active caspase-3 expression compared to other groups. Conclusion: Argon- or xenon-satured cold-storage solution preserved renal architecture and function following transplantation by reducing ischemia-reperfusion injury.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Noble Gas (Argon and Xenon)-Saturated Cold Storage Solutions Reduce Ischemia-Reperfusion Injury in a Rat Model of Renal Transplantation

et al. 2012

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“…Although interpretation of survival is a critical issue, and reports of survival rates following extended cold ischemia of kidneys can vary due to factors such as rat strains used and different surgical techniques [23,24] , variability was reduced in our setting, since all our surgical procedures were performed by one surgeon under standardized conditions. However, comparison to previously reported survival rates remains difficult since removal of the recipient's native right kidney is often not performed until several days after transplantation [24,25] , and in some rat kidney transplantation models that involve bilateral nephrectomy at the time of transplantation, observation periods for survival range from 24 h [26] to 2 weeks [27] .…”

Section: Discussionmentioning

confidence: 99%

Impact of Organ Preservation Using HTK for Graft Flush and Subsequent Storage in UW in Rat Kidney Transplantation

Schmitz

Klawitter²,

Bendrick-Peart³

et al. 2006

Eur Surg Res

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Background: In kidney transplantation, preservation has a significant influence on organ function. Since previous reports have indicated a benefit of combining histidine-tryptophan-ketoglutarate (HTK) and University of Wisconsin (UW) solution, we evaluated the effects of initial flush with low viscosity HTK, followed by storage in UW. Material and Methods: Kidneys from inbred Lewis rats were procured using HTK or UW for initially perfusion and re-flushed after 30 min with either solution. In a third group, after perfusion with HTK, organs were re-flushed with UW. Organs were stored for 16–24 h (4°C). Study parameters were high-energy phosphates, histology, apoptosis, recipient survival and urine excretion of 15-F_2t-isoprostanes (oxidative stress marker). Results:Prior to transplantation, tissue ATP/ADP concentrations were: HTK/UW>UW-only >HTK-only. In transplanted kidneys, histological damage was highest after preservation in HTK-only. Twenty-four hours after transplantation (24 h cold ischemia time – CIT), cleaved-PARP was most abundant using UW-only. 16 h of CIT resulted in higher urine concentrations of isoprostanes in the order HTK-only (368 ± 308) > UW-only (157 ± 105) > HTK/UW (67 ± 26), and was lower in HTK/UW after 24 h of CIT (146 ± 38) vs. UW-only (507 ± 33 pg/mg creatinine). Survival (24 h CIT) was significantly reduced, and percentage of initial non-functioning (INF) kidneys highest in HTK-only (2.6 ± 0.3 days, 100%), compared to UW-only (13 ± 4.4 days, 75%) and HTK/UW (18.5 ± 4.6 days, 33%). Conclusions: In long-term preservation, UW is superior over HTK. However, our results indicate that perfusion with HTK prior to storage in UW may improve the results of UW alone which is reflected by better survival, lower rate of INF, higher cellular energy conservation and a decrease of free radicals.

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“…Cold and hypoxic ischemic injuries of rabbit and dogs kidneys were cured by glycine and glycine treatment improved the graft function transplantation [51]. Moreover, kidneys rinsed in glycine containing Carolina solution can be protected against reperfusion injury or storage injury and enhance renal graft function and long survival after kidney transplantation [52]. The usage of glycine in organ transplantation is most widely investigated in liver transplantation.…”

Section: Beneficial Effects Of Glycinementioning

confidence: 99%

Multifarious Beneficial Effect of Nonessential Amino Acid, Glycine: A Review

Razak

Begum²,

Viswanath

et al. 2017

Oxidative Medicine and Cellular Longevity

218

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Glycine is most important and simple, nonessential amino acid in humans, animals, and many mammals. Generally, glycine is synthesized from choline, serine, hydroxyproline, and threonine through interorgan metabolism in which kidneys and liver are the primarily involved. Generally in common feeding conditions, glycine is not sufficiently synthesized in humans, animals, and birds. Glycine acts as precursor for several key metabolites of low molecular weight such as creatine, glutathione, haem, purines, and porphyrins. Glycine is very effective in improving the health and supports the growth and well-being of humans and animals. There are overwhelming reports supporting the role of supplementary glycine in prevention of many diseases and disorders including cancer. Dietary supplementation of proper dose of glycine is effectual in treating metabolic disorders in patients with cardiovascular diseases, several inflammatory diseases, obesity, cancers, and diabetes. Glycine also has the property to enhance the quality of sleep and neurological functions. In this review we will focus on the metabolism of glycine in humans and animals and the recent findings and advances about the beneficial effects and protection of glycine in different disease states.

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Carolina rinse solution minimizes kidney injury and improves graft function and survival after prolonged cold ischemia1

Abstract: Flushing renal explants with warm CRS before implantation diminishes cold ischemia-reperfusion injury and improves the function and survival of transplanted kidneys.

Cited by 12 publications

References 36 publications

Noble Gas (Argon and Xenon)-Saturated Cold Storage Solutions Reduce Ischemia-Reperfusion Injury in a Rat Model of Renal Transplantation

Noble Gas (Argon and Xenon)-Saturated Cold Storage Solutions Reduce Ischemia-Reperfusion Injury in a Rat Model of Renal Transplantation

Impact of Organ Preservation Using HTK for Graft Flush and Subsequent Storage in UW in Rat Kidney Transplantation

Multifarious Beneficial Effect of Nonessential Amino Acid, Glycine: A Review

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