Carotenoid Production by a Novel Isolate of Microbacterium paraoxydans

Ojha, Swati; Kapoor, Sanjiv; Mishra, Saroj

doi:10.1007/s12088-017-0686-9

Cited by 10 publications

(11 citation statements)

References 22 publications

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“…In addition to the previously mentioned authors, Ojha et al . 28 also detected neurosporene in the Microbacterium genus 28 . However, with the combined information of the UV-Visible spectra mentioned above and the results of APCI-MS, to our knowledge, it was the first time that the extracted sample was compared and matched to a purified neurosporene standard.…”

Section: Resultsmentioning

confidence: 92%

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Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01

Reis-Mansur

Cardoso-Rurr

Silva

et al. 2019

Sci Rep

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The Microbacterium sp. LEMMJ01 isolated from Antarctic soil does not belong to any of the nearest species identified in the RDP database. Under UV radiation (A, B and C wavebands) the survival fractions of Microbacterium sp. cells were much higher compared with wild-type E . coli K12A15. Especially remarkable for an Antarctic bacterium, an expressive resistance against high UV-B doses was observed. The increased survival of DNA repair-proficient E . coli grown overnight added of 0.1 mg/ml or 1 mg/ml of the whole pigment extract produced by Microbacterium sp. revealed that part of the resistance of Microbacterium sp. against UV-B radiation seems to be connected with photoprotection by its pigments. Scanning electron microscopy revealed that UV-A and UV-B ensued membrane alterations only in E . coli . The APCI-MS fingerprints revealed the diagnostic ions for neurosporene (m/z 580, 566, 522, 538, and 524) synergism for the first time in this bacterium by HPLC-MS/MS analysis. Carotenoids also were devoid of phototoxicity and cytotoxicity effects in mouse cells and in human keratinocytes and fibroblasts.

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Section: Resultsmentioning

confidence: 92%

“…Carotenoid pigments from Microbacterium paraoxydans were isolated and characterized by Ojha et al . 28 , and the finding of a neurosporene structure was also proposed.…”

Section: Resultsmentioning

confidence: 96%

Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01

Reis-Mansur

Cardoso-Rurr

Silva

et al. 2019

Sci Rep

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“…Although it is reported that Microbacterium spp. can produce that those pigments (Ojha et al, 2018) among our isolates, none of them showed pigments in the culture conditions.…”

Section: Tablementioning

confidence: 67%

“…In the literature, among the carotenoids found in T. magnatum red patches, Bixin, b-carotene and cis-1-glycosyl-apo-8 0 -lycopene have been associated with Microbacterium spp. (Ojha et al, 2018). This species appears to be the most probable candidate for production, at least in part, of carotenoids in the truffle.…”

Section: Resultsmentioning

confidence: 91%

Microbial and pigment profile of the reddish patch occurring within Tuber magnatum ascomata

et al. 2018

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Tuber magnatum Pico, the delectable white truffle, is the most prized truffle species. In this study, we examined the reddish pigmentation that frequently occurs in T. magnatum ascomata for the presence of pigment-producing bacteria. The inner part of the reddish-pigmented region of three T. magnatum ascomata collected in NortheCentral Italy was analysed. This reddish part was used to establish a bacterial culture collection and to extract the total genomic DNA in order to obtain a library of 16S rRNA genes representative of the bacterial community. The molecular approach revealed limited microbial diversity within the reddishpigmented regions compared to the wider range of bacterial species commonly found at the same maturation stage and season in T. magnatum ascomata. The pigmented regions showed a prevalence of specific bacterial species belonging to a-, band g-Proteobacteria, Actinobacteria and Firmicutes. From the tandem mass spectrometry analysis of the extracted pigment, four compounds were identified: i) bixin, ii) b-carotene, iii) cis-1-glycosyl-apo-8 0-lycopene and iv) the fucoxanthin. Carotenoid producing species such as Microbacterium and Chryseobacterium emerged as the most likely cause of the peculiar reddish pigment production. Indeed, our findings suggest that the peculiar reddish pigment might be produced by these bacterial species.

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“… 76 Pigment production can be modulated by changing the carbon and nitrogen sources and salt concentrations in the growth medium. 77 In the future, we would like to test different carbon and nitrogen sources and salt concentrations to see if we can formulate a growth medium that will enhance decaprenoxanthin production in the stationary phase.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of a heavy metal- and antibiotic-tolerant novel bacterial strain from a contaminated culture plate of Chlamydomonas reinhardtii, a green micro-alga.

et al. 2021

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Background: Chlamydomonas reinhardtii, a green micro-alga, is normally cultured in laboratories in Tris-Acetate Phosphate (TAP), a medium which contains acetate as the sole carbon source. Acetate in TAP can lead to occasional bacterial and fungal contamination. We isolated a yellow-pigmented bacterium from a Chlamydomonas TAP plate. It was named Clip185 based on the Chlamydomonas strain plate it was isolated from. In this article we present our work on the isolation, taxonomic identification and physiological and biochemical characterizations of Clip185. Methods: We measured sensitivities of Clip185 to five antibiotics and performed standard microbiological tests to characterize it. We partially sequenced the 16S rRNA gene of Clip185. We identified the yellow pigment of Clip185 by spectrophotometric analyses. We tested tolerance of Clip185 to six heavy metals by monitoring its growth on Lysogeny Broth (LB) media plates containing 0.5 mM -10 mM concentrations of six different heavy metals. Results: Clip185 is an aerobic, gram-positive rod, oxidase-negative, mesophilic, alpha-hemolytic bacterium. It can ferment glucose, sucrose and mannitol. It is starch hydrolysis-positive. It is very sensitive to vancomycin but resistant to penicillin and other bacterial cell membrane- and protein synthesis-disrupting antibiotics. Clip185 produces a C50 carotenoid, decaprenoxanthin, which is a powerful anti-oxidant with a commercial demand. Decaprenoxanthin production is induced in Clip185 under light. NCBI-BLAST analyses of the partial 16S rRNA gene sequence of Clip185 revealed a 99% sequence identity to that of Microbacterium binotii strain PK1-12M and Microbacterium sp. strain MDP6. Clip185 is able to tolerate toxic concentrations of six heavy metals. Conclusions: Our results show that Clip185 belongs to the genus Microbacterium. In the future, whole genome sequencing of Clip185 will clarify if Clip185 is a new Microbacterium species or a novel strain of Microbacterium binotii, and will reveal its genes involved in antibiotic-resistance, heavy-metal tolerance and regulation of decaprenoxanthin biosynthesis.

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Carotenoid Production by a Novel Isolate of Microbacterium paraoxydans

Cited by 10 publications

References 22 publications

Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01

Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01

Microbial and pigment profile of the reddish patch occurring within Tuber magnatum ascomata

Isolation and characterization of a heavy metal- and antibiotic-tolerant novel bacterial strain from a contaminated culture plate of Chlamydomonas reinhardtii, a green micro-alga.

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