Carotenoid production by Formosa sp. KMW, a marine bacteria of Flavobacteriaceae family: Influence of culture conditions and nutrient composition

Sowmya, R.; Sachindra, N.M.

doi:10.1016/j.bcab.2015.08.018

Cited by 31 publications

(19 citation statements)

References 36 publications

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“…A plausible explanation for the significant improvement in the β-carotene production that the strain utilizes the glucose can easily be absorbed in the biosynthetic pathway of β-carotene. Similarly, Sowmya and Sachindra (2015) supported our result that in the absence of any sugar in the production medium the carotenoids yield was low 115 ± 10.4 whereas it was increased in the presence of glucose (166.1 ± 13.33) by Flavobacteriacea sp. Choudhari and Singhal (2008) found increasing β-carotene (99 ± 2 mg/L) production by the Blakeslea trispora strain in the presence of glucose.…”

Section: Discussionsupporting

confidence: 89%

Optimization of submerged fermentation process for improved production of β-carotene by Exiguobacterium acetylicum S01

Sekar

Kumar

Dahms‬

et al. 2019

Heliyon

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Carotenoids are natural pigments with substantial applications in nutraceutical, pharmaceutical, and food industries. In this study, optimization of the fermentation process for enhancement of β-carotene and biomass production by Exiguobacterium acetylicum S01 was achieved by employing statistical designs including the Placket-Burman design (PBD) and response surface methodology (RSM). Among the seven variables investigated by two levels in PBD, glucose, peptone, pH and temperature were indicated as crucial variables ( p < 0.0001) for β-carotene and biomass productivity. Response surface methodology was further applied to evaluate the optimal concentrations of these four variables for maximum β-carotene and biomass productivity. The optimized medium contained glucose 1.4 g/L, peptone 26.5 g/L, pH 8.5, and temperature 30 °C, respectively. A significant increase in β-carotene (40.32 ± 2.55 mg/L) and biomass (2.19 ± 0.10 g/L) productivities in E. acetylicum S01 were achieved by using RSM, which was 3.47-fold and 2.36-fold higher in the optimized medium compared to the un-optimized medium. Further, the optimum fermentation condition in the 5-L bioreactor was achieved a maximal β-carotene yield of 107.22 ± 5.78 mg/L within 96 h. Moreover, the expression levels of carotenoid biosynthetic genes (phytoene desaturase (CrtI) and phytoene synthase (CrtB)) were up-regulated (2.89-fold and 3.71-fold) in E. acetylicum under the optimized medium conditions. Overall, these results suggest that E. acetylicum S01 can be used as a promising microorganism for the commercial production of β-carotene.

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Section: Discussionsupporting

confidence: 89%

Optimization of submerged fermentation process for improved production of β-carotene by Exiguobacterium acetylicum S01

Sekar

Kumar

Dahms‬

et al. 2019

Heliyon

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“…b Yield vs glucose, inoculum, and c yield vs glucose, pH the addition of glycerol. In a similar work reported by Sowmya and Sachindra (2015), a significant improved carotenoid content was achieved in control (0.70 mg/L) followed by glucose (0.66 mg/L). This is further corroborated in Flavobacterium sp., as although the supplementation of glucose elicited carotenoid production, maximum growth was achieved with sucrose (Alcantara and Sanchez 1999).…”

Section: Process Optimisation Of Culture Conditions For Zeaxanthin Yisupporting

confidence: 78%

“…In aerobic bacteria, aeration had a better impact on zeaxanthin production, as utilization of oxygen occurs in various carotenoid biosynthesis steps, viz. cyclisation, desaturation and oxygenation (Sowmya and Sachindra 2015). Gradual increase was observed in zeaxanthin accumulation as the agitation was increased reaching maximum at 120 and 180 rpm, bacterial carotenoid accumulation and DCW increased when incubated under shaking conditions.…”

Section: Process Optimisation Of Culture Conditions For Zeaxanthin Yimentioning

confidence: 92%

Ameliorating process parameters for zeaxanthin yield in Arthrobacter gandavensis MTCC 25325

et al. 2020

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The present study aims to escalate the production of prophylactic agent zeaxanthin using a screened potential bacterial isolate. For this purpose, a freshwater bacterium capable of producing zeaxanthin was isolated from Bor Talav, Bhavnagar. The 16S rRNA sequence confirmed the isolate as Arthrobacter gandavensis. The bacterium was also submitted to Microbial Type Culture Collection, CSIR-Institute of Microbial Technology, Chandigarh, India, with the accession number MTCC 25325. The chemo-metric tools were employed to optimise the influencing factors such as pH, temperature, inoculum size, agitation speed, carbon source and harvest time on zeaxanthin yield. Thereafter, six parameters were narrowed down to three factors and were optimised using the central composite design (CCD) matrix. Maximum zeaxanthin (1.51 mg/g) was derived when A. gandavensis MTCC 25325 was grown under pH 6.0, 1.5% (w/v) glucose and 10% (v/v) inoculum size. A high regression coefficient (R 2 = 0.92) of the developed model indicated the accurateness of the tested parameters. To the best of our knowledge, this is the first report on tailoring the process parameters using chemo-metric optimisation for escalating the zeaxanthin production by A. gandavensis MTCC 25325.

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“…Addition of sugars to LB medium led to a marginal increase in carotenoid yield (* 1.8 to 3.1-fold) with maximum enhancement in the presence of sucrose. The effect of carbon and nitrogen source has been investigated on carotenoid production in Phaffia rhodozyma and Formosa [12][13][14], Sporidiobolus pararoseus [15] and the results indicate fermentative sugars such as sucrose, mannose to support higher carotenoids formation (of 13.98 mg/L) than glycerol. An increase in TCA cycle intermediate, Acetyl Co-A, resulting from breakdown of sugars, and which is also a precursor of isoprenoid biosynthetic pathway may be responsible for enhancing yield of carotenoids.…”

mentioning

confidence: 99%

“…While glucose has been reported to support good carotenoid yield in S. pararoseus, the present study indicated it to result in lower yield and this was attributed to repression of the relevant biosynthetic pathways. In the marine bacterium, Formosa sp., maximum carotenoids (15 mg/L) were accumulated in the absence of any carbon source [14]. The role of nitrogen source was investigated as several studies [15][16][17][18] indicate that the Nitrogen (inorganic or organic) were added to LB medium to a final concentration of 2.5% (w v -1 ) in the beginning of the experiment concentration and nature of nitrogen source (inorganic or organic) affects total carotene yield.…”

mentioning

confidence: 99%

Carotenoid Production by a Novel Isolate of Microbacterium paraoxydans

2017

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This study reports extraction and characterization of carotenoid pigments from , a non-photosynthetic bacterium, cultivated in Luria-Bertani (LB) medium. The isolate was identified to be moderately halo- and osmo-tolerant capable of withstanding high (~ 6%) salt and sugar (30% w/v sucrose, 20% w/v glucose) concentrations after a brief period of adaptation. The pigments were characterized using a combination of UV-Vis spectral analysis with the λ at 407, 436 and 466 nm and ESI-MS with an value at 536.44. The absorption profile of the pigments and their nature was influenced by carbon, nitrogen source and presence of salt in the growth medium. Highest level of pigment (~ 16 g kg dry wt cells) was produced in NHCl supplemented LB medium. The pigment displayed free radical scavenging, anticancer activity, characteristic of the plant carotenoids. Based on the accumulation of pigments under different conditions, a biochemical pathway for synthesis of neurosporene was proposed.

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Carotenoid production by Formosa sp. KMW, a marine bacteria of Flavobacteriaceae family: Influence of culture conditions and nutrient composition

Cited by 31 publications

References 36 publications

Optimization of submerged fermentation process for improved production of β-carotene by Exiguobacterium acetylicum S01

Optimization of submerged fermentation process for improved production of β-carotene by Exiguobacterium acetylicum S01

Ameliorating process parameters for zeaxanthin yield in Arthrobacter gandavensis MTCC 25325

Carotenoid Production by a Novel Isolate of Microbacterium paraoxydans

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