Carriage of CTX-M-15-Producing
            <i>Escherichia coli</i>
            Isolates among Children Living in a Remote Village in Senegal

Ruppé, Étienne; Woerther, Paul‐Louis; Diop, Abdoulaye; Sene, Anne-Marie; Costa, Annaelle Da; Arlet, Guillaume; Andremont, Antoine; Rouveix, B

doi:10.1128/aac.00139-09

Cited by 46 publications

(41 citation statements)

References 19 publications

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“…These results support previous findings suggesting a wider dissemination of AAC(69)-Ib-cr than Qnr determinants, especially in multiple clones of E. coli carrying the bla CTX-M-15 gene (Pitout et al, 2009;Ruppé et al, 2009;Soge et al, 2006).…”

Section: A Barguigua and Others 1348supporting

confidence: 92%

Characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates from the community in Morocco

Barguigua

Otmani

Talmi

et al. 2011

Journal of Medical Microbiology

104

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Of 803 community Escherichia coli (n5767) and Klebsiella pneumoniae (n536) isolates collected from patients with urinary tract infections in three Moroccan cities, 10 E. coli (1.3%) and 2 K. pneumoniae (5.6 %) isolates were shown to produce extended-spectrum b-lactamases (ESBLs). PFGE revealed that the E. coli isolates comprised seven distinct genotypes. The presence of plasmids in the 12 isolates was revealed by conjugation experiments of plasmids from these Enterobacteriaceae strains with E. coli K 12 J 5 , with further isolation of the plasmids in the transconjugants. Subsequent nucleotide sequencing indicated that the plasmids encoded the bla CTX-M , bla OXA , bla TEM and bla SHV genes, including genes for CTX-M-15 (n511), OXA-1 (n511), TEM-1b (n54), SHV-5 (n51) and SHV-1 (n52). Identification of plasmid-mediated quinolone-resistance genes was performed by PCR. The aac(69)Ib-cr variant was detected in all strains, and two strains co-expressed qnrS1, bla CTX-M-15 and bla OXA-1 genes. The presence of ESBLs in the Enterobacteriaceae strains studied was probably due to the dissemination of resistance plasmids with the predominant genotype of bla CTX-M-15.

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Section: A Barguigua and Others 1348supporting

confidence: 92%

Characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates from the community in Morocco

Barguigua

Otmani

Talmi

et al. 2011

Journal of Medical Microbiology

104

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“…Moreover, ESBL maintenance in the bacterial population may be due to the high levels of use of ampicillin or by co-selection related to the presence in the same genetic structure of mechanisms of resistance for other non-related antimicrobial agents used in the area. In this line of thought, carriage of bla CTX-M-15 -producing E. coli isolates was reported in children living in a remote village with no data known of antibiotic exposure [30]. These data agree with the current massive spread of ESBLs worldwide.…”

Section: Discussionsupporting

confidence: 74%

“…Although ESBLs have been previously reported in sub-Saharan Africa [5][6][7][27][28][29][30], the report of ESBLs in a rural hospital with limited access to third-generation cephalosporins, such as in Manhiça, Mozambique, is relevant, suggesting again the coselection of antibiotic resistance genes. Despite access to thirdgeneration cephalosporins being limited in this community, a high prevalence of b-lactamase genes, such as bla TEM-like and bla OXA-like , has been reported in Shigella and Salmonella isolates in previous studies, reflecting of the high use of ampicillin in the area [23].…”

Section: Discussionmentioning

confidence: 99%

Characterisation of extended-spectrum β-lactamases among Klebsiella pneumoniae isolates causing bacteraemia and urinary tract infection in Mozambique

Pons

Vubil

Guiral

et al. 2015

Journal of Global Antimicrobial Resistance

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“…In our study, the prevalence of ESBL-E. coli carriage in stool was as high as 66.3%. This is much higher than in a 2012 study in northern Cameroon which reported a prevalence of 23.1% among community outpatients [3] or in other African studies which reported rates of 30.9 % in Niger [10], 10% in Senegal [11] and 7.3 % in Tunisia [12]. This is even higher to what has been reported in China where a rate of 50.5% of CTX-M-E. coli [13] was described.…”

Section: Discussioncontrasting

confidence: 56%

Intestinal carriage of Extended Spectrum Beta-Lactamase producing E. coli in women with urinary tract infections, Cameroon

Djuikoue

Woerther

Toukam

et al. 2016

J Infect Dev Ctries

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Introduction: During the last decade, the prevalence of the intestinal carriage of extended spectrum beta-lactamases -producing Escherichia coli (ESBL-E. coli) has continued to increase worldwide in the community, especially in developing countries. Hence, we undertook a study to determine the ESBL-E. coli fecal carriage rate and the associated risk factors in Cameroonian women. Methodology: A total of 86 women suspected of community-acquired urinary tract infections (UTI) were included in 10 health structures from May 2011 to April 2012. After filling a questionnaire, they provided a stool sample that was plated on selective media for ESBL producing bacteria. The identification of strains was obtained with mass spectrometry and the antibiotic susceptibility by disk diffusion in agar media. The ESBL type was determined by PCR. The relative abundance of ESBL-E. coli was measured for positive samples. Eventually, the presence of antibiotics in stool was assessed. Results: The carriage rate of ESBL-E. coli was 57/86 (66.3%). Phenotypic and molecular characterization showed that all ESBL-E. coli strains contained group 1 CTX-M enzymes. Multivariate analysis showed that ESBL-E. coli fecal carriage was associated with the presence of antibiotics in stools (p < 0.05). Although not significant, mean ESBL relative abundance tended to be higher in patients with antibiotic exposure. Conclusions: Our results show that the carriage of ESBL-E. coli fecal carriage in women with UTI suspicion from the Cameroonian community is extremely high and associated with recent antibiotic intake.

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Carriage of CTX-M-15-Producing Escherichia coli Isolates among Children Living in a Remote Village in Senegal

Cited by 46 publications

References 19 publications

Characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates from the community in Morocco

Characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates from the community in Morocco

Characterisation of extended-spectrum β-lactamases among Klebsiella pneumoniae isolates causing bacteraemia and urinary tract infection in Mozambique

Intestinal carriage of Extended Spectrum Beta-Lactamase producing E. coli in women with urinary tract infections, Cameroon

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